{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T11:12:12Z","timestamp":1772795532307,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[1993,5,1]],"date-time":"1993-05-01T00:00:00Z","timestamp":736214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1993,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The gene encoding the B type variable surface protein from Paramecium tetraurelia stock 51 has been cloned and sequenced. The 7,182 nucleotide open reading frame contains no introns and encodes a cysteine-rich protein that has a periodic structure including three nearly perfect tandem repeats in the central region. Interestingly, the B gene is located near a macronuclear telomere as was shown previously for two other paramecium surface protein genes. In this paper, we characterize four independent mutants with complete macronuclear deletions of the B gene. Previous analysis of different macronuclear deletion mutants of the A surface protein gene demonstrated two types of inheritance: typical Mendelian segregation (as illustrated by d12) and cytoplasmic inheritance (shown by d48). F1 analysis of four B- mutants crossed with wild-type cells reveals heterozygous F1 cell lines derived from both parental cytoplasms contain approximately the same copy number of the B gene, as expected for a recessive Mendelian mutation. Analysis of F2 progeny from three of these four B- mutant crosses indicates that one of the three exhibits a Mendelian 1:1 segregation ratio of B+ and B- cell lines. The other two show a preponderance of B+ cells, but this is not correlated with the parental cytoplasmic type. In addition to having a large number of B+ individuals, the d12.144, A-, B- mutant produced some F2 progeny that stably maintain less than normal macronuclear amounts of the A gene and\/or the B gene.<\/jats:p>","DOI":"10.1093\/genetics\/134.1.189","type":"journal-article","created":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T20:12:39Z","timestamp":1610568759000},"page":"189-198","source":"Crossref","is-referenced-by-count":23,"title":["Molecular and genetic analyses of the B type surface protein gene from Paramecium tetraurelia."],"prefix":"10.1093","volume":"134","author":[{"given":"J","family":"Scott","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Leeck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Forney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1993,5,1]]},"container-title":["Genetics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/genetics\/article-pdf\/134\/1\/189\/34593007\/genetics0189.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/genetics\/article-pdf\/134\/1\/189\/34593007\/genetics0189.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T19:22:57Z","timestamp":1619119377000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/genetics\/article\/134\/1\/189\/6011160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,5,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,5,1]]}},"URL":"https:\/\/doi.org\/10.1093\/genetics\/134.1.189","relation":{},"ISSN":["1943-2631"],"issn-type":[{"value":"1943-2631","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1993,5,1]]},"published":{"date-parts":[[1993,5,1]]}}}