{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:37:31Z","timestamp":1791077851918,"version":"4.1.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[1997,12,1]],"date-time":"1997-12-01T00:00:00Z","timestamp":880934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We identified and characterized 14 extragenic mutations that suppressed the dominant egg-laying defect of certain lin-12 gain-of-function mutations. These suppressors defined seven genes: sup-l7, lag-2, sel-4, sel-5, sel-6, sel-7 and sel-8. Mutations in six of the genes are recessive suppressors, whereas the two mutations that define the seventh gene, lag-2, are semi-dominant suppressors. These suppressor mutations were able to suppress other lin-12 gain-of-function mutations. The suppressor mutations arose at a very low frequency per gene, 10-50 times below the typical loss-of-function mutation frequency. The suppressor mutations in sup1 7 and lag-2 were shown to be rare non-null alleles, and we present evidence that null mutations in these two genes cause lethality. Temperature-shift studies for two suppressor genes, sup1 7and lag-2, suggest that both genes act at approximately the same time as lin-12in specifying a cell fate. Suppressor alleles of six of these genes enhanced a temperature-sensitive loss-of-function allele of glp-1, a gene related to lin-12 in structure and function. Our analysis of these suppressors suggests that the majority of these genes are part of a shared lin-12\/glp-1 signal transduction pathway, or act to regulate the expression or stability of lin-12 and glp-1.<\/jats:p>","DOI":"10.1093\/genetics\/147.4.1675","type":"journal-article","created":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T17:55:40Z","timestamp":1610560540000},"page":"1675-1695","source":"Crossref","is-referenced-by-count":41,"title":["Identification and Characterization of Genes That Interact With\n                    <i>lin-12<\/i>\n                    in\n                    <i>Caenorhabditis elegans<\/i>"],"prefix":"10.1093","volume":"147","author":[{"given":"Frans E","family":"Tax","sequence":"first","affiliation":[{"name":"Department of Genetics, University of Washington, Seattle, Washington 98195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James H","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Genetics, University of Washington, Seattle, Washington 98195"},{"name":"Massachusetts Institute of Technology, Cambridge, Massachusetts 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin L","family":"Ferguson","sequence":"additional","affiliation":[{"name":"Massachusetts Institute of Technology, Cambridge, Massachusetts 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H Robert","family":"Horvitzt","sequence":"additional","affiliation":[{"name":"Massachusetts Institute of Technology, Cambridge, Massachusetts 02139"},{"name":"Howard Hughes Medical Institute, Department of Biology, Cambridge, Massachusetts 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1997,12,1]]},"container-title":["Genetics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/genetics\/article-pdf\/147\/4\/1675\/37016398\/genetics1675.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/genetics\/article-pdf\/147\/4\/1675\/37016398\/genetics1675.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T15:46:18Z","timestamp":1619538378000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/genetics\/article\/147\/4\/1675\/6054074"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,12,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1997,12,1]]}},"URL":"https:\/\/doi.org\/10.1093\/genetics\/147.4.1675","relation":{},"ISSN":["1943-2631"],"issn-type":[{"value":"1943-2631","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1997,12,1]]},"published":{"date-parts":[[1997,12,1]]}}}