{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T15:27:14Z","timestamp":1791127634762,"version":"4.1.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1998,6,1]],"date-time":"1998-06-01T00:00:00Z","timestamp":896659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We show that major chromosomal rearrangements can occur upon T-DNA transformation of Arabidopsis thaliana. In the ACL4 line, two T-DNA insertion loci were found; one is a tandem T-DNA insert in a head-to-head orientation, and the other is a truncated insert with only the left part of the T-region. The four flanking DNA regions were isolated and located on the Arabidopsis chromosomes; for both inserts, one side of the T-DNA maps to chromosome 2, whereas the other side maps to chromosome 3. Both chromosome 3 flanking regions map to the same location, despite a 1.4-kb deletion at this point, whereas chromosome 2 flanking regions are located 40 cM apart on the bottom arm of chromosome 2. These results strongly suggest a reciprocal translocation between chromosomes 2 and 3, with the breakpoints located at the T-DNA insertion sites. The interchanged fragments roughly correspond to the 20-cM distal ends of both chromosomes. Moreover, a large inversion, spanning 40 cM on the genetic map, occurs on the bottom arm of chromosome 2. This was confirmed by genetic analyses that demonstrated a strong reduction of recombination in the inverted region. Models for T-DNA integration and the consequences for T-DNA tagging are discussed in light of these results.<\/jats:p>","DOI":"10.1093\/genetics\/149.2.641","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T02:05:29Z","timestamp":1619575529000},"page":"641-650","source":"Crossref","is-referenced-by-count":148,"title":["Major Chromosomal Rearrangements Induced by T-DNA Transformation in Arabidopsis"],"prefix":"10.1093","volume":"149","author":[{"given":"Philippe","family":"Nacry","sequence":"first","affiliation":[{"name":"Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Camilleri","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9atrice","family":"Courtial","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Caboche","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Bouchez","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1998,6,1]]},"reference":[{"key":"2022010310202232600_R1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.3109\/10520296909063335","article-title":"Differential staining of aborted and non aborted pollen","volume":"44","author":"Alexander","year":"1969","journal-title":"Stain Technol."},{"key":"2022010310202232600_R2","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S0168-9525(97)01094-9","article-title":"T-DNA insertion mutagenesis in Arabidopsis: going back and forth","volume":"13","author":"Azpiroz-Leehan","year":"1997","journal-title":"Trends Genet."},{"key":"2022010310202232600_R3","first-page":"1194","article-title":"In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants","volume":"316","author":"Bechtold","year":"1993","journal-title":"C. 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Genet."},{"key":"2022010310202232600_R7","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1007\/BF00041406","article-title":"Details of T-DNA structural organization from a transgenic Petunia population exhibiting co-suppression","volume":"32","author":"Cluster","year":"1996","journal-title":"Plant Mol. 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