{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T08:01:25Z","timestamp":1790150485807,"version":"4.0.1"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[1998,7,1]],"date-time":"1998-07-01T00:00:00Z","timestamp":899251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>\u201cSelective DNA pooling\u201d accomplishes quantitative trait locus (QTL) mapping through densitometric estimates of marker allele frequencies in pooled DNA samples of phenotypically extreme individuals. With poly(TG) microsatellites, such estimates are confounded by \u201cshadow\u201d (\u201cstutter\u201d) bands. A correction procedure was developed on the basis of an observed linear regression between shadow band intensity and allele TG repeat number. Using this procedure, a selective DNA pooling study with respect to milk protein percentage was implemented in Israel-Holstein dairy cattle. Pools were prepared from milk samples of high and low daughters of each of seven sires and genotyped with respect to 11 markers. Highly significant associations with milk protein percentage were found for 5 of the markers; 4 of these markers confirmed previous reports. Selective DNA pooling accessed 80.6 and 48.3%, respectively, of the information that would have been available through individual selective genotyping or total population genotyping. In effect, the statistical power of 45,600 individual genotypings was obtained from 328 pool genotypings. This methodology can make genome-wide mapping of QTL accessible to moderately sized breeding organizations.<\/jats:p>","DOI":"10.1093\/genetics\/149.3.1557","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T01:44:13Z","timestamp":1619574253000},"page":"1557-1567","source":"Crossref","is-referenced-by-count":72,"title":["Quantitative Trait Locus Mapping in Dairy Cattle by Means of Selective Milk DNA Pooling Using Dinucleotide Microsatellite Markers: Analysis of Milk Protein Percentage"],"prefix":"10.1093","volume":"149","author":[{"given":"Ehud","family":"Lipkin","sequence":"first","affiliation":[{"name":"Department of Genetics, Alexander Silberman Institute of Life Science, Hebrew University of Jerusalem, Jerusalem 91904, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias O","family":"Mosig","sequence":"additional","affiliation":[{"name":"Department of Genetics, Alexander Silberman Institute of Life Science, Hebrew University of Jerusalem, Jerusalem 91904, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariel","family":"Darvasi","sequence":"additional","affiliation":[{"name":"Department of Genetics, Alexander Silberman Institute of Life Science, Hebrew University of Jerusalem, Jerusalem 91904, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ephraim","family":"Ezra","sequence":"additional","affiliation":[{"name":"Israel Cattle Breeder's Association, Caesaria, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Shalom","sequence":"additional","affiliation":[{"name":"Department of Genetics, Alexander Silberman Institute of Life Science, Hebrew University of Jerusalem, Jerusalem 91904, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Friedmann","sequence":"additional","affiliation":[{"name":"Department of Genetics, Alexander Silberman Institute of Life Science, Hebrew University of Jerusalem, Jerusalem 91904, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morris","family":"Soller","sequence":"additional","affiliation":[{"name":"Department of Genetics, Alexander Silberman Institute of Life Science, Hebrew University of Jerusalem, Jerusalem 91904, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1998,7,1]]},"reference":[{"key":"2022010309452814200_R1","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/ng0394-227","article-title":"A genetic linkage map of the bovine genome","volume":"6","author":"Barendse","year":"1994","journal-title":"Nature Genetics"},{"key":"2022010309452814200_R2","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1093\/genetics\/136.2.619","article-title":"A genetic linkage map for cattle","volume":"136","author":"Bishop","year":"1994","journal-title":"Genetics"},{"key":"2022010309452814200_R3","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.3382\/ps.0741855","article-title":"Development of a genetic map of the chicken with markers of high utility","volume":"74","author":"Cheng","year":"1995","journal-title":"Poult. Sci."},{"key":"2022010309452814200_R4","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1093\/genetics\/140.2.703","article-title":"An autosomal genetic linkage map of the sheep genome","volume":"140","author":"Crawford","year":"1995","journal-title":"Genetics"},{"key":"2022010309452814200_R5","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s003359900353","article-title":"The effect of selective genotyping on QTL mapping accuracy","volume":"8","author":"Darvasi","year":"1997","journal-title":"Mamm. Genome"},{"key":"2022010309452814200_R6","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/BF00222881","article-title":"Selective genotyping for determination of linkage between a marker locus and a quantitative trait locus","volume":"85","author":"Darvasi","year":"1992","journal-title":"Theor. Appl. Genet."},{"key":"2022010309452814200_R7","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1093\/genetics\/138.4.1365","article-title":"Selective DNA pooling for determination of linkage between a molecular marker and a quantitative trait locus","volume":"138","author":"Darvasi","year":"1994","journal-title":"Genetics"},{"key":"2022010309452814200_R8","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1023\/A:1025685324830","article-title":"A simple method to calculate resolving power and cofidence interval of QTL map location","volume":"27","author":"Darvasi","year":"1997","journal-title":"Behav. Genet."},{"key":"2022010309452814200_R9","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1038\/380152a0","article-title":"Comprehensive genetic map of the human genome based on 5,264 microsatellites","volume":"380","author":"Dib","year":"1996","journal-title":"Nature"},{"key":"2022010309452814200_R10","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/380149a0","article-title":"A comprehensive genetic map of the mouse genome","volume":"380","author":"Dietrich","year":"1996","journal-title":"Nature"},{"key":"2022010309452814200_R11","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1073\/pnas.90.3.1058","article-title":"Microsatellite mapping of the gene causing weaver disease in cattle will allow the study of an associated quantitative trait locus","volume":"91","author":"George","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2022010309452814200_R12","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1093\/genetics\/139.2.907","article-title":"Mapping quantitative trait loci controlling milk production in dairy cattle by exploiting progeny testing","volume":"139","author":"George","year":"1995","journal-title":"Genetics"},{"key":"2022010309452814200_R13","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1093\/hmg\/2.4.411","article-title":"A study of the origin of \u2018shadow bands\u2019 seen when typing dinucleotide repeat polymorphisms by the PCR","volume":"2","author":"Hauge","year":"1993","journal-title":"Hum. Mol. Genet."},{"key":"2022010309452814200_R14","first-page":"63","article-title":"Marker-assisted selection of candidate bulls for progeny testing programs","volume":"51","author":"Kashi","year":"1990","journal-title":"Anim. Prod."},{"key":"2022010309452814200_R15","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1101\/gr.4.1.13","article-title":"Determining relative microsatellite allele frequencies in pooled DNA samples","volume":"4","author":"Khatib","year":"1994","journal-title":"PCR Methods Appl."},{"issue":"Suppl.","key":"2022010309452814200_R16","first-page":"103","article-title":"Detection of QTL for milk production traits on chromosome 6 in German Holstein Friesian cattle","volume":"27","author":"K\u00fchn","year":"1996","journal-title":"Anim. Genetics"},{"key":"2022010309452814200_R17","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1093\/genetics\/142.3.945","article-title":"Association between SCCP haplotypes at the bovine growth hormone gene and milk protein percentage","volume":"142","author":"Lagziel","year":"1996","journal-title":"Genetics"},{"key":"2022010309452814200_R18","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1038\/ng1195-241","article-title":"Genetic dissection of complex traits: guidelines for interpreting and reporting linkage results","volume":"11","author":"Lander","year":"1995","journal-title":"Nature Genet."},{"key":"2022010309452814200_R19","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1101\/gr.4.6.331","article-title":"Batched analysis of genotypes","volume":"4","author":"Leduc","year":"1995","journal-title":"PCR Methods Appl."},{"key":"2022010309452814200_R20","first-page":"203","article-title":"Slipped-strand mispairing: a major mechanism for DNA sequence evolution","volume":"4","author":"Levinson","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"2022010309452814200_R21","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.3168\/jds.S0022-0302(93)77536-0","article-title":"Milk as a source of deoxyribonucleic acid and as a substrate for the polymerase chain reaction","volume":"76","author":"Lipkin","year":"1993","journal-title":"J. Dairy Sci."},{"key":"2022010309452814200_R22","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1080\/10495399309525796","article-title":"Early PCR amplification test for identifying chimerism in female calves co-twin to a male in cattle","volume":"4","author":"Lipkin","year":"1993","journal-title":"Anim. Biotech."},{"key":"2022010309452814200_R23","unstructured":"Lipkin\n              E\n            \n          , 1996 \u00a0Use of pooled DNA samples for the estimation of microsatellites allele frequencies by direct PCR from milk samples. Ph.D. Thesis (in Hebrew with English summary), Hebrew University, Jerusalem."},{"key":"2022010309452814200_R24","first-page":"280","article-title":"Shadow bands seen when typing polymorphic dinucleotide repeats: some causes and cures","volume":"15","author":"Litt","year":"1993","journal-title":"BioTechnology"},{"key":"2022010309452814200_R25","first-page":"105","article-title":"Weaver carrier status effects on yield in German Brown cattle","volume":"27","author":"Medjugorac","year":"1996","journal-title":"Anim. Genet."},{"key":"2022010309452814200_R26","doi-asserted-by":"crossref","first-page":"2395","DOI":"10.1093\/nar\/21.10.2395","article-title":"The determination of the sequences present in the shadow bands of a dinucleotide repeat PCR","volume":"21","author":"Murray","year":"1993","journal-title":"Nucl. Acids Res."},{"key":"2022010309452814200_R27","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1186\/BF03548647","article-title":"Differential cell counting in fraction-collected milk from dairy cows","volume":"29","author":"Ostensson","year":"1988","journal-title":"Acta Vet. Scand."},{"key":"2022010309452814200_R28","first-page":"1199","article-title":"Toward fully automated genotyping: genotyping microsatellite markers by deconvolution","volume":"57","author":"Perlin","year":"1995","journal-title":"Am. J. Hum. Genet."},{"key":"2022010309452814200_R29","doi-asserted-by":"crossref","first-page":"676","DOI":"10.3168\/jds.S0022-0302(96)76413-5","article-title":"Misidentification rate in the Israeli dairy cattle population and its implication for genetic improvement","volume":"79","author":"Ron","year":"1996","journal-title":"J. Dairy Sci."},{"issue":"Suppl.","key":"2022010309452814200_R30","first-page":"105","article-title":"Detection of individual loci affecting economic traits in the US Holstein population with the aid of DNA microsatellites","volume":"27","author":"Ron","year":"1996","journal-title":"Anim. Genet."},{"key":"2022010309452814200_R31","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1111\/j.1365-2052.1993.tb00324.x","article-title":"Dinucleotide repeat polymorphism at the bovine locus HUJ616","volume":"24","author":"Shalom","year":"1993","journal-title":"Anim. Genet."},{"key":"2022010309452814200_R32","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1111\/j.1365-2052.1994.tb00063.x","article-title":"Dinucleotide repeat polymorphism at the bovine HUJII77, HUJ223, HUJVI174, HUJ175 loci","volume":"25","author":"Shalom","year":"1994","journal-title":"Anim. Genet."},{"key":"2022010309452814200_R33","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.3168\/jds.S0022-0302(90)78949-7","article-title":"Genetic mapping of the bovine genome using DNA-level markers with particular attention toloci affecting quantitative traits of economic importance","volume":"73","author":"Soller","year":"1990","journal-title":"J. Dairy Sci."},{"key":"2022010309452814200_R34","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1080\/10495399409525821","article-title":"Marker assisted selection\u2014an overview","volume":"5","author":"Soller","year":"1994","journal-title":"Anim. Biotech."},{"key":"2022010309452814200_R35","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/BF00277402","article-title":"On the power of experimental designs for detection of linkage between marker loci and quantitative loci in crosses between inbred lines","volume":"47","author":"Soller","year":"1976","journal-title":"Theor. Appl. Genet."},{"key":"2022010309452814200_R36","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1093\/genetics\/144.4.1799","article-title":"Quantitative trait loci analysis for five milk production traits on chromosome six in the Dutch Holstein-Friesian population","volume":"144","author":"Spelman","year":"1996","journal-title":"Genetics"},{"key":"2022010309452814200_R37","doi-asserted-by":"crossref","first-page":"6463","DOI":"10.1093\/nar\/17.16.6463","article-title":"Hypervariability of simple sequences as a general source for polymorphic DNA markers","volume":"17","author":"Tautz","year":"1989","journal-title":"Nucl. Acids Res."},{"key":"2022010309452814200_R38","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1111\/j.1365-2052.1995.tb02694.x","article-title":"Case in haplotypes and their association with milk production traits in the Finnish Ayrshire cattle","volume":"26","author":"Velmala","year":"1995","journal-title":"Anim. Genet."},{"key":"2022010309452814200_R39","first-page":"375","volume-title":"Probability and Statistics for Engineers and Scientists","author":"Walpole","year":"1972"},{"key":"2022010309452814200_R40","first-page":"388","article-title":"Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction","volume":"44","author":"Weber","year":"1989","journal-title":"Am. J. Hum. Genet."},{"key":"2022010309452814200_R41","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.3168\/jds.S0022-0302(90)78938-2","article-title":"Power of daughter and granddaughter designs for determining linkage between marker loci and quantitative trait loci in dairy cattle","volume":"73","author":"Weller","year":"1990","journal-title":"J. Dairy Sci."},{"key":"2022010309452814200_R42","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/0959-437X(95)80004-O","article-title":"Bovine genome mapping: evolutionary inference and the power of comparative genomics","volume":"5","author":"Womack","year":"1995","journal-title":"Curr. Op. Genet. Devel."}],"container-title":["Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/genetics\/article-pdf\/149\/3\/1557\/42007948\/genetics1557.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/genetics\/article-pdf\/149\/3\/1557\/42007948\/genetics1557.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,3]],"date-time":"2022-01-03T09:49:03Z","timestamp":1641203343000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/genetics\/article\/149\/3\/1557\/5923039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,7,1]]},"references-count":42,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1998,7,1]]},"published-print":{"date-parts":[[1998,7,1]]}},"URL":"https:\/\/doi.org\/10.1093\/genetics\/149.3.1557","relation":{},"ISSN":["1943-2631"],"issn-type":[{"value":"1943-2631","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1998,7,1]]},"published":{"date-parts":[[1998,7,1]]}}}