{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:15:35Z","timestamp":1789661735890,"version":"4.0.1"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[1999,11,1]],"date-time":"1999-11-01T00:00:00Z","timestamp":941414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1999,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Caenorhabditis elegans lin-3 encodes a homolog of the epidermal growth factor (EGF) family of growth factors. LIN-3 is the inductive signal for hermaphrodite vulval differentiation, and it is required for animal viability, hermaphrodite fertility, and the specification of anterior cell fates in the male B cell lineage. We describe the cloning of a lin-3 homolog from C. briggsae, sequence comparison of C. elegans lin-3 with C. briggsae lin-3, and the determination of molecular lesions in alleles of C. elegans lin-3, including three new alleles. We also analyzed the severity of phenotypes caused by the new and existing alleles of lin-3. Correlation of mutant phenotypes and their molecular lesions, as well as sequence comparison between two species, reveal that the EGF motif and the N-terminal portion of the cytoplasmic domain are important for the functions of LIN-3 in all tissues, while the C-terminal portion of the cytoplasmic domain is involved in the tissue-specific functions of lin-3. We discuss how the structure of lin-3 contributes to its functions in multiple developmental processes.<\/jats:p>","DOI":"10.1093\/genetics\/153.3.1257","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T01:09:25Z","timestamp":1619572165000},"page":"1257-1269","source":"Crossref","is-referenced-by-count":21,"title":["Structural Requirements for the Tissue-Specific and Tissue-General Functions of the Caenorhabditis elegans Epidermal Growth Factor LIN-3"],"prefix":"10.1093","volume":"153","author":[{"given":"Jing","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phoebe","family":"Tzou","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Division of Biology, California Institute of Technology, Pasadena, California 91125"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell J","family":"Hill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul W","family":"Sternberg","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Division of Biology, California Institute of Technology, Pasadena, California 91125"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1999,11,1]]},"reference":[{"key":"2022010403525477100_R1","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1038\/348693a0","article-title":"The let-23 gene necessary for Caenorhabditis elegans vulval induction encodes a tyrosine kinase of the EGF receptor subfamily","volume":"348","author":"Aroian","year":"1990","journal-title":"Nature"},{"key":"2022010403525477100_R2","first-page":"626","article-title":"Splicing in Caenorhabditis elegans does not require an AG at the 3\u2032 splice acceptor site","volume":"13","author":"Aroian","year":"1993","journal-title":"Mol. 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