{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T16:08:17Z","timestamp":1704902897123},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2000,12,1]],"date-time":"2000-12-01T00:00:00Z","timestamp":975628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The lin-31 gene is required for the proper specification of vulval cell fates in the nematode Caenorhabditis elegans and encodes a member of the winged-helix family of transcription factors. Members of this important family have been identified in many organisms and are known to bind specific DNA targets involved in a variety of developmental processes. DNA sequencing of 13 lin-31 alleles revealed six nonsense mutations and two missense mutations within the DNA-binding domain, plus three deletions, one transposon insertion, and one frameshift mutation that all cause large-scale disruptions in the gene. The missense mutations are amino acid substitutions in the DNA-binding domain and probably disrupt interactions of the LIN-31 transcription factor with its DNA target. In addition, detailed phenotypic analysis of all 19 alleles showed similar penetrances for several characteristics examined. From our analysis we conclude: (1) the null phenotype of lin-31 is the phenotype displayed by almost all of the existing alleles, (2) the DNA-binding domain plays a critical role in LIN-31 function, and (3) direct screens for multivulva and vulvaless mutants will probably yield only null (or strong) alleles of lin-31.<\/jats:p>","DOI":"10.1093\/genetics\/156.4.1595","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T03:57:19Z","timestamp":1619582239000},"page":"1595-1602","source":"Crossref","is-referenced-by-count":14,"title":["Null Mutations in the <i>lin-31<\/i> Gene Indicate Two Functions During <i>Caenorhabditis elegans<\/i> Vulval Development"],"prefix":"10.1093","volume":"156","author":[{"given":"Leilani M","family":"Miller","sequence":"first","affiliation":[{"name":"Department of Biology, Santa Clara University, Santa Clara, California 95053"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather A","family":"Hess","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David B","family":"Doroquez","sequence":"additional","affiliation":[{"name":"Department of Biology, Santa Clara University, Santa Clara, California 95053"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noelle M","family":"Andrews","sequence":"additional","affiliation":[{"name":"Department of Biology, Santa Clara University, Santa Clara, California 95053"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2000,12,1]]},"reference":[{"key":"2022010407090669300_R1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/0092-8674(94)90522-3","article-title":"HNF-3\u03b2 is essential for node and notochord formation in mouse development","volume":"78","author":"Ang","year":"1994","journal-title":"Cell"},{"key":"2022010407090669300_R2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1006\/dbio.1996.0219","article-title":"A fork head\/HNF-3 homolog expressed in the pharynx and intestine of the Caenorhabditis elegans embryo","volume":"178","author":"Azzaria","year":"1996","journal-title":"Dev. 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