{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T11:38:58Z","timestamp":1779277138729,"version":"3.51.4"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2001,4,1]],"date-time":"2001-04-01T00:00:00Z","timestamp":986083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We determined that a mutation in the nucleoporin gene NUP170 leads to defects in chromosome transmission fidelity (ctf) and kinetochore integrity in Saccharomyces cerevisiae. A ctf mutant strain, termed s141, shows a transcription readthrough phenotype and stabilizes a dicentric chromosome fragment in two assays for kinetochore integrity. Previously, these assays led to the identification of two essential kinetochore components, Ctf13p and Ctf14p. Thus, s141 represents another ctf mutant involved in the maintenance of kinetochore integrity. We cloned and mapped the gene complementing the ctf mutation of s141 and showed that it is identical to the S. cerevisiae NUP170 gene. A deletion strain of NUP170 (nup170\u0394::HIS3) has a Ctf\u2013 phenotype similar to the s141 mutant (nup170-141) and also exhibits a kinetochore integrity defect. We identified a second nucleoporin, NUP157, a homologue of NUP170, as a suppressor of the Ctf\u2013 phenotype of nup170-141 and nup170\u0394::HIS3 strains. However, a deletion of NUP157 or several other nucleoporins did not affect chromosome segregation. Our data suggest that NUP170 encodes a specialized nucleoporin with a unique role in chromosome segregation and possibly kinetochore function.<\/jats:p>","DOI":"10.1093\/genetics\/157.4.1543","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T03:48:43Z","timestamp":1619581723000},"page":"1543-1553","source":"Crossref","is-referenced-by-count":46,"title":["Novel Role for a <i>Saccharomyces cerevisiae<\/i> Nucleoporin, Nup170p, in Chromosome Segregation"],"prefix":"10.1093","volume":"157","author":[{"given":"Oliver","family":"Kerscher","sequence":"first","affiliation":[{"name":"Department of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20889"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip","family":"Hieter","sequence":"additional","affiliation":[{"name":"Center for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, British Columbia V5Z4H4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Winey","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Munira A","family":"Basrai","sequence":"additional","affiliation":[{"name":"Department of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20889"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,4,1]]},"reference":[{"key":"2022010422141912500_R1","volume-title":"Methods in Yeast Genetics","author":"Adams","year":"1997"},{"key":"2022010422141912500_R2","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1083\/jcb.131.5.1133","article-title":"Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear poremembrane protein Pom152p","volume":"131","author":"Aitchison","year":"1995","journal-title":"J. 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