{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:23:17Z","timestamp":1791231797552,"version":"4.1.0"},"reference-count":63,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2001,5,1]],"date-time":"2001-05-01T00:00:00Z","timestamp":988675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Like many other plant disease resistance genes, Arabidopsis thaliana RPS2 encodes a product with nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domains. This study explored the hypothesized interaction of RPS2 with other host factors that may be required for perception of Pseudomonas syringae pathogens that express avrRpt2 and\/or for the subsequent induction of plant defense responses. Crosses between Arabidopsis ecotypes Col-0 (resistant) and Po-1 (susceptible) revealed segregation of more than one gene that controls resistance to P. syringae that express avrRpt2. Many F2 and F3 progeny exhibited intermediate resistance phenotypes. In addition to RPS2, at least one additional genetic interval associated with this defense response was identified and mapped using quantitative genetic methods. Further genetic and molecular genetic complementation experiments with cloned RPS2 alleles revealed that the Po-1 allele of RPS2 can function in a Col-0 genetic background, but not in a Po-1 background. The other resistance-determining genes of Po-1 can function, however, as they successfully conferred resistance in combination with the Col-0 allele of RPS2. Domain-swap experiments revealed that in RPS2, a polymorphism at six amino acids in the LRR region is responsible for this allele-specific ability to function with other host factors.<\/jats:p>","DOI":"10.1093\/genetics\/158.1.439","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T05:37:44Z","timestamp":1619588264000},"page":"439-450","source":"Crossref","is-referenced-by-count":61,"title":["The Leucine-Rich Repeat Domain Can Determine Effective Interaction Between <i>RPS2<\/i> and Other Host Factors in Arabidopsis <i>RPS2<\/i>-Mediated Disease Resistance"],"prefix":"10.1093","volume":"158","author":[{"given":"Diya","family":"Banerjee","sequence":"first","affiliation":[{"name":"Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706"},{"name":"Program in Physiological and Molecular Plant Biology, University of Illinois, Urbana, Illinois 61801"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Crop Sciences, University of Illinois, Urbana, Illinois 61801"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew F","family":"Bent","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706"},{"name":"Program in Physiological and Molecular Plant Biology, University of Illinois, Urbana, Illinois 61801"},{"name":"Department of Crop Sciences, University of Illinois, Urbana, Illinois 61801"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,5,1]]},"reference":[{"key":"2022010506090579200_R1","volume-title":"Current Protocols in Molecular Biology","author":"Ausubel","year":"1997"},{"key":"2022010506090579200_R2","first-page":"1","article-title":"A strategy involving the use of high redundancy YAC subclone libraries facilitates the contiguous representation in cosmid and BAC clones of 1.7 Mb of the genome of the plant Arabidopsis thaliana","volume":"4","author":"Bancroft","year":"1997","journal-title":"Weeds World"},{"key":"2022010506090579200_R3","first-page":"1194","article-title":"In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants","volume":"316","author":"Bechtold","year":"1993","journal-title":"C. 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