{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T12:18:53Z","timestamp":1774786733383,"version":"3.50.1"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2001,6,1]],"date-time":"2001-06-01T00:00:00Z","timestamp":991353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>An intensive linkage map of the yellow fever mosquito, Aedes aegypti, was constructed using single-strand conformation polymorphism (SSCP) analysis of cDNA markers to identify single nucleotide polymorphisms (SNPs). A total of 94 A. aegypti cDNAs were downloaded from GenBank and primers were designed to amplify fragments &amp;lt;500 bp in size. These primer pairs amplified 94 loci, 57 (61%) of which segregated in a single F1 intercross family among 83 F2 progeny. This allowed us to produce a dense linkage map of one marker every 2 cM distributed over a total length of 134 cM. Many A. aegypti cDNAs were highly similar to genes in the Drosophila melanogaster genome project. Comparative linkage analysis revealed areas of synteny between the two species. SNP polymorphisms are abundant in A. aegypti genes and should prove useful in both population genetics and mapping studies.<\/jats:p>","DOI":"10.1093\/genetics\/158.2.715","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T04:03:15Z","timestamp":1619582595000},"page":"715-726","source":"Crossref","is-referenced-by-count":49,"title":["SSCP Analysis of cDNA Markers Provides a Dense Linkage Map of the <i>Aedes aegypti<\/i> Genome"],"prefix":"10.1093","volume":"158","author":[{"given":"Ruth E","family":"Fulton","sequence":"first","affiliation":[{"name":"Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael L","family":"Salasek","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy M","family":"DuTeau","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"IV","given":"William C","family":"Black","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,6,1]]},"reference":[{"key":"2022010505240545900_R1","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.1126\/science.287.5461.2185","article-title":"The genome sequence of Drosophila melanogaster","volume":"287","author":"Adams","year":"2000","journal-title":"Science"},{"key":"2022010505240545900_R2","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1093\/genetics\/143.4.1727","article-title":"Intensive linkage mapping in a wasp (Bracon hebetor) and a mosquito (Aedes aegypti) with single-strand conformation polymorphism analysis of random amplified polymorphic DNA markers","volume":"143","author":"Antolin","year":"1996","journal-title":"Genetics"},{"key":"2022010505240545900_R3","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/978-94-009-1535-0_31","article-title":"RAPD-PCR and SSCP analysis for insect population genetic studies","volume-title":"The Molecular Biology of Insect Disease Vectors: A Methods Manual","author":"Black","year":"1997"},{"key":"2022010505240545900_R4","first-page":"438","article-title":"Molecular taxonomy and systematics of arthropod vectors","volume-title":"Biology of Disease Vectors","author":"Black","year":"1996"},{"key":"2022010505240545900_R5","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1093\/genetics\/156.2.687","article-title":"Quantitative trait loci that control vector competence for dengue-2 virus in the mosquito Aedes aegypti","volume":"156","author":"Bosio","year":"2000","journal-title":"Genetics"},{"key":"2022010505240545900_R6","first-page":"67","article-title":"Genetics of Aedes aegypti","volume-title":"Genetics of Insect Vectors of Disease","author":"Craig","year":"1967"},{"key":"2022010505240545900_R7","first-page":"527","article-title":"Genetic variability in populations of Aedes aegypti","volume":"24","author":"Craig","year":"1961","journal-title":"Bull. 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