{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T19:19:55Z","timestamp":1782587995030,"version":"3.54.5"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2001,8,1]],"date-time":"2001-08-01T00:00:00Z","timestamp":996624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>C57BL\/6J-T-associated sex reversal (B6-TAS) in XY mice results in ovarian development and involves (1) hemizygosity for Tas, a gene located in the region of Chromosome 17 deleted in Thp and TOrl, (2) homozygosity for one or more B6-derived autosomal genes, and (3) the presence of the AKR Y chromosome. Here we report results from experiments designed to investigate the Y chromosome component of this sex reversal. Testis development was restored in B6 TOrl\/+XYAKR mice carrying a Mus musculus Sry transgene. In addition, two functionally different classes of M. domesticus Sry alleles were identified among eight standard and two wild-derived inbred strains. One class, which includes AKR, did not initiate normal testis development in B6 TOrl\/+ XY mice, whereas the other did. DNA sequence analysis of the Sry ORF and a 5\u2032 800-bp segment divided these inbred strains into the same groups. Finally, we found that Sry is transcribed in B6 TOrl\/+ XYAKR fetal gonads but at a reduced level. These results pinpoint Sry as the Y-linked component of B6-TAS. We hypothesize that the inability of specific M. domesticus Sry alleles to initiate normal testis development in B6 TOrl\/+ XYAKR mice results from a biologically insufficient level of Sry expression, allowing the ovarian development pathway to proceed.<\/jats:p>","DOI":"10.1093\/genetics\/158.4.1675","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T04:06:36Z","timestamp":1619582796000},"page":"1675-1681","source":"Crossref","is-referenced-by-count":35,"title":["C57BL\/6J-<i>T<\/i>-Associated Sex Reversal in Mice Is Caused by Reduced Expression of a <i>Mus domesticus Sry<\/i> Allele"],"prefix":"10.1093","volume":"158","author":[{"given":"Linda L","family":"Washburn","sequence":"first","affiliation":[{"name":"The Jackson Laboratory, Bar Harbor, Maine 04609"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth H","family":"Albrecht","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory, Bar Harbor, Maine 04609"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva M","family":"Eicher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,8,1]]},"reference":[{"key":"2022010506245811500_R1","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1093\/genetics\/147.3.1267","article-title":"DNA Sequence analysis of Sry alleles (subgenus Mus) implicates misregulation as the cause of C57BL\/6J-YPOS sex reversal and defines the SRY functional unit","volume":"147","author":"Albrecht","year":"1997","journal-title":"Genetics"},{"key":"2022010506245811500_R2","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1006\/dbio.2000.9819","article-title":"Defective mesonephric cell migration is associated with abnormal testis cord development in C57BL\/6JMus domesticus mice","volume":"225","author":"Albrecht","year":"2000","journal-title":"Dev. Biol."},{"key":"2022010506245811500_R3","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1006\/dbio.1994.1018","article-title":"Embryonic expression of Lim-1, the mouse homolog of Xenopus Xlim-1, suggests a role in lateral mesoderm differentiation and neurogenesis","volume":"161","author":"Barnes","year":"1994","journal-title":"Dev. Biol."},{"key":"2022010506245811500_R4","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1017\/S0016672300015883","article-title":"Observations on a set of radiation-induced dominant T-like mutations in the mouse","volume":"26","author":"Bennett","year":"1975","journal-title":"Genet. Res."},{"key":"2022010506245811500_R5","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1139\/g94-041","article-title":"The testis-determining autosomal trait, Tda-1, of C57BL\/6J is determined by more than a single autosomal gene when compared with DBA\/2J mice","volume":"37","author":"Biddle","year":"1994","journal-title":"Genome"},{"key":"2022010506245811500_R6","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S0925-4773(99)00047-7","article-title":"Migration of mesonephric cells into the mammalian gonad depends on Sry","volume":"84","author":"Capel","year":"1999","journal-title":"Mech. Dev."},{"key":"2022010506245811500_R7","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1038\/ng0394-245","article-title":"Polymorphism of a CAG trinucleotide repeat within Sry correlates with B6.YDOM sex reversal","volume":"6","author":"Coward","year":"1994","journal-title":"Nat. Genet."},{"key":"2022010506245811500_R8","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1098\/rstb.1988.0118","article-title":"Autosomal genes involved in mammalian primary sex determination","volume":"322","author":"Eicher","year":"1988","journal-title":"Philos. Trans. R Soc. Lond. B Biol. Sci."},{"key":"2022010506245811500_R9","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1073\/pnas.75.2.946","article-title":"Assignment of genes to regions of mouse chromosomes","volume":"75","author":"Eicher","year":"1978","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2022010506245811500_R10","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1002\/jez.1402280213","article-title":"Inherited sex reversal in mice: identification of a new primary sex-determining gene","volume":"228","author":"Eicher","year":"1983","journal-title":"J. Exp. Zool."},{"key":"2022010506245811500_R11","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1159\/000131518","article-title":"A cytogenetic investigation of inherited true hermaphroditism in BALB\/cWt mice","volume":"28","author":"Eicher","year":"1980","journal-title":"Cytogenet. Cell. Genet."},{"key":"2022010506245811500_R12","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1126\/science.7089579","article-title":"Mus poschiavinus Y chromosome in the C57BL\/6J murine genome causes sex reversal","volume":"217","author":"Eicher","year":"1982","journal-title":"Science"},{"key":"2022010506245811500_R13","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1093\/genetics\/122.1.181","article-title":"A repeated segment on the mouse Y chromosome is composed of retroviral-related, Y-enriched, and Y-specific sequences","volume":"122","author":"Eicher","year":"1989","journal-title":"Genetics"},{"key":"2022010506245811500_R14","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1098\/rstb.1995.0160","article-title":"Sex reversal in C57BL\/6J-YPOS mice corrected by a Sry transgene","volume":"350","author":"Eicher","year":"1995","journal-title":"Philos. Trans. R Soc. Lond. B Biol. Sci."},{"key":"2022010506245811500_R15","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1038\/ng1096-206","article-title":"Sex-determining genes on mouse autosomes identified by linkage analysis of C57BL\/6J-YPOS sex reversal","volume":"14","author":"Eicher","year":"1996","journal-title":"Nat. Genet."},{"key":"2022010506245811500_R16","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/274163a0","article-title":"Deletion mapping of the t complex of chromosome 17 of the mouse","volume":"274","author":"Erickson","year":"1978","journal-title":"Nature"},{"key":"2022010506245811500_R17","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1006\/bbrc.1995.1384","article-title":"Sequence variation in the 5\u2032, putative promoter of Sry and its possible relevance to the C57BL\/6J-YDOM sex reversal","volume":"208","author":"Graves","year":"1995","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2022010506245811500_R18","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1038\/346245a0","article-title":"A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes","volume":"346","author":"Gubbay","year":"1990","journal-title":"Nature"},{"key":"2022010506245811500_R19","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1006\/geno.1993.1291","article-title":"High-resolution comparative mapping of mouse chromosome 17","volume":"17","author":"Himmelbauer","year":"1993","journal-title":"Genomics"},{"key":"2022010506245811500_R20","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1038\/ng0895-480","article-title":"Expression of a linear Sry transcript in the mouse genital ridge","volume":"10","author":"Jeske","year":"1995","journal-title":"Nat. Genet."},{"key":"2022010506245811500_R21","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1038\/342940a0","article-title":"Zfy gene expression patterns are not compatible with a primary role in mouse sex determination","volume":"342","author":"Koopman","year":"1989","journal-title":"Nature"},{"key":"2022010506245811500_R22","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/351117a0","article-title":"Male development of chromosomally female mice transgenic for Sry","volume":"352","author":"Koopman","year":"1991","journal-title":"Nature"},{"key":"2022010506245811500_R23","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1016\/S0960-9822(06)00415-5","article-title":"Male-specific cell migration into the developing gonad","volume":"7","author":"Martineau","year":"1997","journal-title":"Curr. Biol."},{"key":"2022010506245811500_R24","first-page":"38","volume":"48","author":"Moutier","year":"1973","journal-title":"Mouse News Lett"},{"key":"2022010506245811500_R25","first-page":"42","volume":"49","author":"Moutier","year":"1973","journal-title":"Mouse News Lett."},{"key":"2022010506245811500_R26","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1111\/j.1432-0436.1987.tb01560.x","article-title":"Morphological development of the mouse gonad in tda-1 XY sex reversal","volume":"33","author":"Nagamine","year":"1987","journal-title":"Differentiation"},{"key":"2022010506245811500_R27","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1006\/dbio.1999.9436","article-title":"Sex reversal caused by Mus musculus domesticus Y chromosomes linked to variant expression of the testis-determining gene Sry","volume":"216","author":"Nagamine","year":"1999","journal-title":"Dev. Biol."},{"key":"2022010506245811500_R28","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1242\/dev.113.2.709","article-title":"The Mus musculus domesticus Tdy allele acts later than the Mus musculus musculus Tdy allele: a basis for XY sex-reversal in C57BL\/6-YPOSmice","volume":"113","author":"Palmer","year":"1991","journal-title":"Development"},{"key":"2022010506245811500_R29","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1038\/346240a0","article-title":"A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif","volume":"346","author":"Sinclair","year":"1990","journal-title":"Nature"},{"key":"2022010506245811500_R30","doi-asserted-by":"crossref","first-page":"2883","DOI":"10.1242\/dev.126.13.2883","article-title":"Mesonephric cell migration induces testis cord formation and Sertoli cell differentiation in the mammalian gonad","volume":"126","author":"Tilmann","year":"1999","journal-title":"Development"},{"key":"2022010506245811500_R31","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1007\/BF00292153","article-title":"Geographic origin of the Y chromosome of \u201cold\u201d inbred strains of mice","volume":"3","author":"Tucker","year":"1992","journal-title":"Mamm. 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