{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:16:36Z","timestamp":1789661796580,"version":"4.0.1"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>To compare vulva development mechanisms in the nematode Oscheius sp. 1 to those known in Caenorhabditis elegans, we performed a genetic screen for vulva mutants in Oscheius sp. 1 CEW1. Here we present one large category of mutations that we call cov, which affect the specification of the Pn.p ventral epidermal cells along the antero-posterior axis. The Pn.p cells are numbered from 1 to 12 from anterior to posterior. In wild-type Oscheius sp. 1 CEW1, the P(4-8).p cells are competent to form the vulva and the progeny of P(5-7).p actually form the vulva, with the descendants of P6.p adopting a central vulval fate. Among the 17 mutations (defining 13 genes) that we characterize here, group 1 mutations completely or partially abolish P(4-8).p competence, and this correlates with early fusion of the Pn.p cells to the epidermal syncytium. In this group, we found a putative null mutation in the lin-39 HOM-C homolog, the associated phenotype of which could be weakly mimicked by injection of a morpholino against Osp1-lin-39 in the mother\u2019s germ line. Using cell ablation in a partially penetrant competence mutant, we show that vulval competence is partially controlled by a gonadal signal. Most other mutants found in the screen display phenotypes unknown in C. elegans. Group 2 mutants show a partial penetrance of Pn.p competence loss and an abnormal centering of the vulva on P5.p, suggesting that these two processes are coregulated by the same pathway in Oscheius sp. 1. Group 3 mutants display an enlarged competence group that includes P3.p, thus demonstrating the existence of a specific mechanism inhibiting P3.p competence. Group 4 mutants display an abnormal centering of the vulval pattern on P7.p and suggest that a specific mechanism centers the vulval pattern on a single Pn.p cell.<\/jats:p>","DOI":"10.1093\/genetics\/163.1.133","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T01:10:43Z","timestamp":1619140243000},"page":"133-146","source":"Crossref","is-referenced-by-count":34,"title":["Control of Vulval Competence and Centering in the Nematode\n                    <i>Oscheius<\/i>\n                    sp. 1 CEW1"],"prefix":"10.1093","volume":"163","author":[{"given":"Sophie","family":"Louvet-Vall\u00e9e","sequence":"first","affiliation":[{"name":"Institut Jacques Monod, CNRS-Universit\u00e9s Paris 6 et 7, 75251 Paris Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Kolotuev","sequence":"additional","affiliation":[{"name":"Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Podbilewicz","sequence":"additional","affiliation":[{"name":"Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Anne","family":"F\u00e9lix","sequence":"additional","affiliation":[{"name":"Institut Jacques Monod, CNRS-Universit\u00e9s Paris 6 et 7, 75251 Paris Cedex 05, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,1,1]]},"reference":[{"key":"2022010518292782800_R1","doi-asserted-by":"crossref","first-page":"3865","DOI":"10.1242\/dev.124.19.3865","article-title":"Maternal control of a zygotic patterning gene in Caenorhabditis elegans","volume":"124","author":"Ahringer","year":"1997","journal-title":"Development"},{"key":"2022010518292782800_R2","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1242\/dev.128.10.1793","article-title":"REF-1, a protein with two bHLH domains, alters the pattern of cell fusion in C. elegans by regulating Hox protein activity","volume":"128","author":"Alper","year":"2001","journal-title":"Development"},{"key":"2022010518292782800_R3","doi-asserted-by":"crossref","first-page":"3335","DOI":"10.1242\/dev.129.14.3335","article-title":"The zinc finger protein REF-2 functions with the Hox genes to inhibit cell fusion in the ventral epidermis of C. elegans","volume":"129","author":"Alper","year":"2002","journal-title":"Development"},{"key":"2022010518292782800_R4","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1006\/dbio.1996.8471","article-title":"Caenorhabditis elegans HOM-C genes regulate the response of vulval precursor cells to inductive signal","volume":"182","author":"Clandinin","year":"1997","journal-title":"Dev. Biol."},{"key":"2022010518292782800_R5","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/0092-8674(93)90293-Y","article-title":"Control of cell fates in the central body region of C. elegans by the homeobox gene lin-39","volume":"74","author":"Clark","year":"1993","journal-title":"Cell"},{"key":"2022010518292782800_R6","unstructured":"Delattre\n              M\n            \n          , 2001 \u00a0Variants de d\u00e9veloppement et \u00e9volution chez les n\u00e9matodes: \u00e9tudes sur la chiralit\u00e9 de P11\/12 et sur le lignage des cellules pr\u00e9curseurs de la vulve. Ph.D. Thesis, Universit\u00e9 de Paris 6, Paris."},{"key":"2022010518292782800_R7","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1006\/dbio.2001.0175","article-title":"Evolution and development of a variable left-right asymmetry in nematodes: the handedness of P11\/P12 migration","volume":"232","author":"Delattre","year":"2001","journal-title":"Dev. Biol."},{"key":"2022010518292782800_R8","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1002\/bies.1116","article-title":"Microevolutionary studies in nematodes: a beginning","volume":"23","author":"Delattre","year":"2001","journal-title":"Bioessays"},{"key":"2022010518292782800_R9","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/S0960-9822(01)00202-0","article-title":"Polymorphism and evolution of vulval precursor cell lineages within two nematode genera, Caenorhabditis and Oscheius","volume":"11","author":"Delattre","year":"2001","journal-title":"Curr. Biol."},{"key":"2022010518292782800_R10","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1093\/genetics\/157.1.183","article-title":"Control of vulval cell division number in the nematode Oscheius\/Dolichorhabditis sp. 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Biol."},{"key":"2022010518292782800_R17","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1163\/156854100508809","article-title":"Comparative developmental studies using Oscheius\/Dolichorhabditis sp. CEW1 (Rhabditidae)","volume":"2","author":"F\u00e9lix","year":"2000","journal-title":"Nematology"},{"key":"2022010518292782800_R18","first-page":"104","article-title":"Three biological species related to Rhabditis (Oscheius) pseudodolichura K\u00f6rner in Osche, 1952","volume":"33","author":"F\u00e9lix","year":"2001","journal-title":"J. Nematol."},{"key":"2022010518292782800_R19","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1093\/genetics\/110.1.17","article-title":"Identification and characterization of 22 genes that affect the vulval cell lineages of Caenorhabditis elegans","volume":"110","author":"Ferguson","year":"1985","journal-title":"Genetics"},{"key":"2022010518292782800_R20","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/326259a0","article-title":"A genetic pathway for the specification of the vulval cell lineages of Caenorhabditis elegans","volume":"326","author":"Ferguson","year":"1987","journal-title":"Nature"},{"key":"2022010518292782800_R21","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/0092-8674(90)90493-X","article-title":"The unc-86 gene product couples cell lineage and cell identity in Caenorhabditis elegans","volume":"63","author":"Finney","year":"1990","journal-title":"Cell"},{"key":"2022010518292782800_R22","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1083\/jcb.114.3.465","article-title":"Muscle cell attachment in Caenorhabditis elegans","volume":"114","author":"Francis","year":"1991","journal-title":"J. Cell Biol."},{"key":"2022010518292782800_R23","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.1101\/gad.200601","article-title":"Functional comparison of the nematode Hox gene lin-39 in C. elegans and P. pacificus reveals evolutionary conservation of protein function despite divergence of primary sequence","volume":"15","author":"Grandien","year":"2001","journal-title":"Genes Dev."},{"key":"2022010518292782800_R24","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1101\/gad.14.7.874","article-title":"The Caenorhabditis elegans APC-related gene apr-1 is required for epithelial cell migration and Hox gene expression","volume":"14","author":"Hoier","year":"2000","journal-title":"Genes Dev."},{"key":"2022010518292782800_R25","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1038\/351535a0","article-title":"Multiple intercellular signalling systems control the development of the C. elegans vulva","volume":"351","author":"Horvitz","year":"1991","journal-title":"Nature"},{"key":"2022010518292782800_R26","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1093\/genetics\/96.2.435","article-title":"Isolation and genetic characterization of cell-lineage mutants of the nematode Caenorhabditis elegans","volume":"96","author":"Horvitz","year":"1980","journal-title":"Genetics"},{"key":"2022010518292782800_R27","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1242\/dev.128.2.253","article-title":"The nematode even-skipped homolog vab-7 regulates gonad and vulva position in Pristionchus pacificus","volume":"128","author":"Jungblut","year":"2001","journal-title":"Development"},{"key":"2022010518292782800_R28","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/0012-1606(79)90035-6","article-title":"Post-embryonic cell lineages of the hermaphrodite and male gonads in Caenorhabditis elegans","volume":"70","author":"Kimble","year":"1979","journal-title":"Dev. 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Dyn."},{"key":"2022010518292782800_R36","doi-asserted-by":"crossref","first-page":"3136","DOI":"10.1101\/gad.251202","article-title":"LIN-39\/Hox triggers cell division and represses EFF-1\/fusogen-dependent vulval cell fusion","volume":"15","author":"Shemer","year":"2002","journal-title":"Genes Dev."},{"key":"2022010518292782800_R37","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1006\/dbio.2000.9657","article-title":"Ring formation drives invagination of the vulva in C. elegans: Ras, cell fusion and cell migration determine structural fates","volume":"221","author":"Shemer","year":"2000","journal-title":"Dev. Biol."},{"key":"2022010518292782800_R38","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1242\/dev.124.1.243","article-title":"Evolutionary changes of developmental mechanisms in the absence of cell lineage alterations during vulva formation in the Diplogastridae (Nematoda)","volume":"124","author":"Sommer","year":"1997","journal-title":"Development"},{"key":"2022010518292782800_R39","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1006\/dbio.1995.1007","article-title":"Evolution of cell lineage and pattern formation in the vulval equivalence group of rhabditid nematodes","volume":"167","author":"Sommer","year":"1995","journal-title":"Dev. Biol."},{"key":"2022010518292782800_R40","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/S0960-9822(02)00421-9","article-title":"Apoptosis and change of competence limit the size of the vulva equivalence group in Pristionchus pacificus: a genetic analysis","volume":"6","author":"Sommer","year":"1996","journal-title":"Curr. 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Biol."},{"key":"2022010518292782800_R48","volume-title":"The Nematode Caenorhabditis elegans","author":"Wood","year":"1988"},{"key":"2022010518292782800_R49","doi-asserted-by":"crossref","first-page":"2875","DOI":"10.1242\/dev.124.15.2875","article-title":"The role of lin-22, a hairy\/Enhancer of split homolog, in patterning the peripheral nervous system of C. elegans","volume":"124","author":"Wrischnik","year":"1997","journal-title":"Development"}],"container-title":["Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/genetics\/article-pdf\/163\/1\/133\/42050124\/genetics0133.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/genetics\/article-pdf\/163\/1\/133\/42050124\/genetics0133.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T13:34:11Z","timestamp":1641389651000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/genetics\/article\/163\/1\/133\/6052880"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1,1]]},"references-count":49,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2003,1,1]]},"published-print":{"date-parts":[[2003,1,1]]}},"URL":"https:\/\/doi.org\/10.1093\/genetics\/163.1.133","relation":{},"ISSN":["1943-2631"],"issn-type":[{"value":"1943-2631","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2003,1,1]]},"published":{"date-parts":[[2003,1,1]]}}}