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These vast arrays of repetitive ribosomal DNA (rDNA) have been implicated not just in ribosome biogenesis, but also aging, cancer, genome stability, and global gene expression. rDNA copy number is highly variable among and within species; this variability is thought to associate with traits relevant to human health and disease. Here we investigate the phenotypic consequences of multicellular life at the lower bounds of rDNA copy number. We use the model Caenorhabditis elegans, which has previously been found to complete embryogenesis using only maternally provided ribosomes. We find that individuals with rDNA copy number reduced to \u223c5% of wild type are capable of further development with variable penetrance. Such individuals are sterile and exhibit severe morphological defects, particularly in post-embryonically dividing tissues such as germline and vulva. Developmental completion and fertility are supported by an rDNA copy number \u223c10% of wild type, with substantially delayed development. Worms with rDNA copy number reduced to \u223c33% of wild type display a subtle developmental timing defect that was absent in worms with higher copy numbers. Our results support the hypothesis that rDNA requirements vary across tissues and indicate that the minimum rDNA copy number for fertile adulthood is substantially less than the lowest naturally observed total copy number. The phenotype of individuals with severely reduced rDNA copy number is highly variable in penetrance and presentation, highlighting the need for continued investigation into the biological consequences of rDNA copy number variation.<\/jats:p>","DOI":"10.1093\/genetics\/iyad039","type":"journal-article","created":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T09:24:33Z","timestamp":1678872273000},"source":"Crossref","is-referenced-by-count":18,"title":["Substantial rDNA copy number reductions alter timing of development and produce variable tissue-specific phenotypes in\n                    <i>C. elegans<\/i>"],"prefix":"10.1093","volume":"224","author":[{"given":"Elizabeth A","family":"Morton","sequence":"first","affiliation":[{"name":"Department of Biology, SUNY Brockport , Brockport, NY 14420 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley N","family":"Hall","sequence":"additional","affiliation":[{"name":"Wisconsin Energy Institute, University of Wisconsin-Madison , Madison, WI 53726 , USA"},{"name":"Molecular and Cellular Biology Program, University of Washington , Seattle, WA 98195 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josh T","family":"Cuperus","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington , Seattle, WA 98195 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0905-4705","authenticated-orcid":false,"given":"Christine","family":"Queitsch","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington , Seattle, WA 98195 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,3,15]]},"reference":[{"issue":"2","key":"2024091718032780100_iyad039-B1","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1093\/genetics\/137.2.483","article-title":"A screen for genetic loci required for 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