{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T04:55:40Z","timestamp":1784955340588,"version":"3.55.0"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T00:00:00Z","timestamp":1742342400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIGMS R35GM134930"],"award-info":[{"award-number":["NIGMS R35GM134930"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM156525"],"award-info":[{"award-number":["R35GM156525"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>De novo genes can be defined as sequences producing evolutionarily derived transcripts that are not homologous to transcripts produced in an ancestor. While they appear to be taxonomically widespread, there is little agreement regarding their abundance, their persistence times in genomes, the population genetic processes responsible for their spread or loss, or their possible functions. In Drosophila melanogaster, 2 approaches have been used to discover these genes and investigate their properties. One uses traditional comparative approaches and existing genomic resources and annotations. A second approach uses raw transcriptome data to discover unannotated genes for which there is no evidence of presence in related species. Investigations using the second approach have focused on D. melanogaster genotypes from recently established cosmopolitan populations. However, most of the genetic variation in the species is found in African populations, suggesting the possibility that fuller understanding of genetic novelties in the species may follow from studies of these populations. Here, we investigate de novo gene candidates expressed in testis and accessory glands in a sample of flies from Zambia and compare them with candidate de novo genes expressed in North American populations. We report a large number of previously undiscovered de novo gene candidates, most of which are expressed polymorphically. Many are predicted to code for secreted proteins. In spite of much different levels of genomic variation in Zambian and North American populations, they express similar numbers of candidate de novo genes. We find evidence from genetic analysis of Raleigh inbred lines that a fraction of rarely expressed gene candidates in this population represent deleterious transcription promoted by inbreeding depression. Many de novo gene candidates are expressed in multiple tissues and both sexes, raising questions about how they may interact with natural selection. The relative importance of positive and negative selection, however, remains unclear.<\/jats:p>","DOI":"10.1093\/genetics\/iyaf044","type":"journal-article","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T18:26:30Z","timestamp":1742408790000},"source":"Crossref","is-referenced-by-count":3,"title":["New perspectives on <i>Drosophila melanogaster<\/i> de novo gene origination revealed by investigation of ancient African genetic variation"],"prefix":"10.1093","volume":"230","author":[{"given":"Julie M","family":"Cridland","sequence":"first","affiliation":[{"name":"Department of Evolution and Ecology, University of California, Davis , Davis, CA 95616 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3763-537X","authenticated-orcid":false,"given":"Elizabeth S","family":"Polston","sequence":"additional","affiliation":[{"name":"Department of Evolution and Ecology, University of California, Davis , Davis, CA 95616 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J","family":"Begun","sequence":"additional","affiliation":[{"name":"Department of Evolution and Ecology, University of California, Davis , Davis, CA 95616 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,3,19]]},"reference":[{"issue":"5","key":"2025050805512072200_iyaf044-B1","doi-asserted-by":"publisher","first-page":"e201900429","DOI":"10.26508\/lsa.201900429","article-title":"Detecting sequence signals in targeting peptides using deep learning","volume":"2","author":"Armenteros","year":"2019","journal-title":"Life Sci Alliance."},{"issue":"6446","key":"2025050805512072200_iyaf044-B2","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1038\/365548a0","article-title":"African and North American Populations of Drosophila melanogaster are very different at the DNA 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