{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T23:42:46Z","timestamp":1787701366087,"version":"build-2784847793"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000057","name":"NIH National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM148158"],"award-info":[{"award-number":["R35GM148158"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Meier\u2013Gorlin syndrome (MGS) is a rare autosomal recessive disorder characterized by microtia, primordial dwarfism, and skeletal abnormalities. Patients with MGS often carry mutations in genes encoding the subunits of the origin recognition complex (ORC), components of the prereplicative complex and replication machinery. ORC6, an essential ORC subunit, plays a critical role in both DNA replication and cytokinesis. Approximately 30% of reported ORC6-related MGS cases exhibit compound heterozygosity for the ORC6 variants c.2T &amp;gt; C (p.Met1Thr) and c.449 + 5G &amp;gt; A. The c.2T &amp;gt; C mutation disrupts the start ATG codon by changing it to ACG, potentially initiating translation at an alternative downstream in-frame Methionine (Met20), while c.449 + 5G &amp;gt; A results in in-frame exon skipping. Both mutations are predicted to produce significantly truncated ORC6 proteins with impaired functionality. In this study, using a humanized ORC6-based Drosophila model, we demonstrate that these truncated proteins fail to rescue orc6 deletion. Instead, our findings reveal that the strong Kozak sequence, naturally present in human ORC6 mRNA, promotes translation from a noncanonical ACG codon. Rescued flies demonstrated a phenotype that we observed earlier for other MGS mutants in Drosophila. These results provide compelling evidence that MGS patients with c.2T &amp;gt; C\/c.449 + 5G &amp;gt; A mutation rely on full size ORC6 protein initiated from a noncanonical ACG start codon.<\/jats:p>","DOI":"10.1093\/genetics\/iyaf203","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T18:09:58Z","timestamp":1758650998000},"source":"Crossref","is-referenced-by-count":1,"title":["Unexpected molecular mechanism of Orc6-based Meier\u2013Gorlin syndrome: insights from a humanized\n                    <i>Drosophila<\/i>\n                    model"],"prefix":"10.1093","volume":"231","author":[{"given":"Maxim","family":"Balasov","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, The University of Alabama at Birmingham, School of Medicine , Birmingham, AL 35294 , United 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