{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T17:28:19Z","timestamp":1782840499752,"version":"3.54.5"},"reference-count":100,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35 GM140857"],"award-info":[{"award-number":["R35 GM140857"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Multiple tissue stem cells depend on glycolysis or \u03b2-oxidation for cell fate decisions. However, how universal these requirements are and how they change as stem cell daughters undergo differentiation remains unclear. The Drosophila ovary is a powerful stem cell model with two distinct stem cell populations: germline stem cells (GSCs), which produce oocytes to perpetuate the species, and follicle stem cells (FSCs), a somatic lineage. Several studies have begun addressing the roles of metabolism within the Drosophila female GSC lineage, but direct systematic analyses of glycolysis and\/or mitochondrial fatty acid \u03b2-oxidation requirements across these lineages have been lacking. Here, using genetic mosaic analysis with null alleles, we found that genes encoding key regulatory glycolytic enzymes\u2014Phosphofructokinase (Pfk) and Pyruvate kinase (Pyk)\u2014are not cell autonomously required for GSC maintenance, proliferation, or early differentiation through 16-cell germline cyst formation and oocyte specification. Although germline cysts lacking Pfk or Pyk function can develop through early vitellogenesis, they grow slowly and display impaired nurse cell chromatin dispersal. By contrast, FSCs and their early daughters require Pfk (but not Pyk) for normal survival, while later follicle cells need both Pfk and Pyk for survival and only Pfk for proliferation, suggesting that follicle cells predominantly require glycolytic intermediates upstream of Pyk. Surprisingly, mitochondrial \u03b2-oxidation was dispensable in both lineages. These findings uncover an unusual metabolic state in GSCs and their early daughters, with marked differences from the neighboring FSC lineage and other somatic stem cells.<\/jats:p>","DOI":"10.1093\/genetics\/iyag015","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T18:30:42Z","timestamp":1768933842000},"source":"Crossref","is-referenced-by-count":3,"title":["Glycolysis is intrinsically required in the follicle stem cell lineage but not in female germline stem cells or their early progeny in\n                    <i>Drosophila melanogaster<\/i>"],"prefix":"10.1093","volume":"232","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2811-681X","authenticated-orcid":false,"given":"Emily M","family":"Wessel","sequence":"first","affiliation":[{"name":"Department of Genetics, University of Wisconsin\u2013Madison , Madison, WI 53706 ,","place":["United States"]},{"name":"Morgridge Institute for Research , Madison, WI 53706 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7330-457X","authenticated-orcid":false,"given":"Daniela","family":"Drummond-Barbosa","sequence":"additional","affiliation":[{"name":"Department of Genetics, University of Wisconsin\u2013Madison , Madison, WI 53706 ,","place":["United States"]},{"name":"Morgridge Institute for Research , Madison, WI 53706 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,1,20]]},"reference":[{"key":"2026030400563677800_iyag015-B1","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1016\/j.stem.2010.10.001","article-title":"The steroid hormone ecdysone functions with intrinsic chromatin remodeling factors to control female germline stem cells in Drosophila","volume":"7","author":"Ables","year":"2010","journal-title":"Cell Stem Cell"},{"key":"2026030400563677800_iyag015-B2","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1002\/wdev.48","article-title":"Control of adult stem cells in vivo by a dynamic physiological environment: diet-dependent systemic factors in Drosophila and beyond","volume":"1","author":"Ables","year":"2012","journal-title":"Wiley Interdiscip Rev Dev Biol"},{"key":"2026030400563677800_iyag015-B3","volume-title":"Molecular Biology of the Cell","author":"Alberts","year":"2002","edition":"4th ed"},{"key":"2026030400563677800_iyag015-B4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/acel.13191","article-title":"Aging shifts mitochondrial dynamics toward fission to promote germline stem cell loss","volume":"19","author":"Amartuvshin","year":"2020","journal-title":"Aging Cell"},{"key":"2026030400563677800_iyag015-B5","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/0167-5877(95)92833-J","article-title":"Extending the interpretation and utility of mixed effects logistic regression models","volume":"24","author":"Atwill","year":"1995","journal-title":"Prev Vet Med"},{"key":"2026030400563677800_iyag015-B6","doi-asserted-by":"publisher","first-page":"786","DOI":"10.1016\/j.tcb.2022.02.003","article-title":"Regulation of gene expression by glycolytic and gluconeogenic enzymes","volume":"32","author":"Bian","year":"2022","journal-title":"Trends Cell Biol"},{"key":"2026030400563677800_iyag015-B7","doi-asserted-by":"publisher","first-page":"958","DOI":"10.1038\/s42255-020-0266-x","article-title":"Cellular metabolic reprogramming controls sugar appetite in Drosophila","volume":"2","author":"Carvalho-Santos","year":"2020","journal-title":"Nat Metab"},{"key":"2026030400563677800_iyag015-B8","doi-asserted-by":"publisher","first-page":"a040535","DOI":"10.1101\/cshperspect.a040535","article-title":"Glycolysis","volume":"13","author":"Chandel","year":"2021","journal-title":"Cold Spring Harb Perspect Biol"},{"key":"2026030400563677800_iyag015-B9","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1038\/ncb3385","article-title":"Metabolic regulation of stem cell function in tissue homeostasis and organismal ageing","volume":"18","author":"Chandel","year":"2016","journal-title":"Nat Cell Biol"},{"key":"2026030400563677800_iyag015-B10","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1053\/j.gastro.2019.11.031","article-title":"HNF4 regulates fatty acid oxidation and is required for renewal of intestinal stem cells in mice","volume":"158","author":"Chen","year":"2020","journal-title":"Gastroenterology"},{"key":"2026030400563677800_iyag015-B11","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.devcel.2020.02.015","article-title":"Glycolysis-independent glucose metabolism distinguishes TE from ICM fate during mammalian embryogenesis","volume":"53","author":"Chi","year":"2020","journal-title":"Dev Cell"},{"key":"2026030400563677800_iyag015-B12","doi-asserted-by":"publisher","first-page":"1579","DOI":"10.1242\/dev.00365","article-title":"A Balbiani body and the fusome mediate mitochondrial inheritance during Drosophila oogenesis","volume":"130","author":"Cox","year":"2003","journal-title":"Development"},{"key":"2026030400563677800_iyag015-B13","doi-asserted-by":"publisher","first-page":"100947","DOI":"10.1016\/j.cois.2022.100947","article-title":"A role for glia in cellular and systemic metabolism\u202f: insights from the fly","volume":"53","author":"De Backer","year":"2022","journal-title":"Curr Opin Insect Sci"},{"key":"2026030400563677800_iyag015-B14","doi-asserted-by":"publisher","first-page":"2781","DOI":"10.1242\/dev.125.15.2781","article-title":"Morphogenesis of the Drosophila fusome and its implications for oocyte specification","volume":"125","author":"de Cuevas","year":"1998","journal-title":"Development"},{"key":"2026030400563677800_iyag015-B15","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1242\/dev.126.2.293","article-title":"The endocycle controls nurse cell polytene chromosome structure during Drosophila oogenesis","volume":"126","author":"Dej","year":"1999","journal-title":"Development"},{"key":"2026030400563677800_iyag015-B16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.7554\/eLife.56922","article-title":"Differentiating Drosophila female germ cells initiate Polycomb silencing by regulating PRC2-interacting proteins","volume":"9","author":"Deluca","year":"2020","journal-title":"Elife"},{"key":"2026030400563677800_iyag015-B17","doi-asserted-by":"publisher","first-page":"710","DOI":"10.1038\/s41556-019-0332-3","article-title":"Mitochondrial fusion regulates lipid homeostasis and stem cell maintenance in the Drosophila testis","volume":"21","author":"Demarco","year":"2019","journal-title":"Nat Cell Biol"},{"key":"2026030400563677800_iyag015-B18","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fcell.2019.00061","article-title":"When place matters\u202f: shuttling of enolase-1 across cellular compartments","volume":"7","author":"Didiasova","year":"2019","journal-title":"Front Cell Dev Biol"},{"key":"2026030400563677800_iyag015-B19","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.immuni.2020.09.014","article-title":"Modification of proteins by metabolites in immunity","volume":"54","author":"Diskin","year":"2021","journal-title":"Immunity"},{"key":"2026030400563677800_iyag015-B20","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1534\/genetics.119.300234","article-title":"Local and physiological control of germline stem cell lineages in Drosophila melanogaster","volume":"213","author":"Drummond-Barbosa","year":"2019","journal-title":"Genetics"},{"key":"2026030400563677800_iyag015-B21","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1006\/dbio.2000.0135","article-title":"Stem cells and their progeny respond to nutritional changes during Drosophila oogenesis","volume":"231","author":"Drummond-Barbosa","year":"2001","journal-title":"Dev Biol"},{"key":"2026030400563677800_iyag015-B22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.7554\/eLife.49050","article-title":"The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cells","volume":"8","author":"Fadiga","year":"2019","journal-title":"Elife"},{"key":"2026030400563677800_iyag015-B23","doi-asserted-by":"publisher","first-page":"1017","DOI":"10.1038\/ncb3575","article-title":"Lactate dehydrogenase activity drives hair follicle stem cell activation","volume":"19","author":"Flores","year":"2017","journal-title":"Nat Cell Biol"},{"key":"2026030400563677800_iyag015-B24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fcell.2020.596819","article-title":"Mitochondrial dynamics in the Drosophila ovary regulates germ stem cell number, cell fate, and female fertility","volume":"8","author":"Garcez","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"2026030400563677800_iyag015-B25","doi-asserted-by":"publisher","first-page":"1213","DOI":"10.1002\/glia.23772","article-title":"Neuronal lactate levels depend on glia-derived lactate during high brain activity in Drosophila","volume":"68","author":"Gonz\u00e1lez-Guti\u00e9rrez","year":"2020","journal-title":"Glia"},{"key":"2026030400563677800_iyag015-B26","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1007\/s00018-014-1739-4","article-title":"Metabolic control of oocyte development: linking maternal nutrition and reproductive outcomes","volume":"72","author":"Gu","year":"2015","journal-title":"Cell Mol Life Sci"},{"key":"2026030400563677800_iyag015-B27","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1002\/mrd.20945","article-title":"Pyruvate and oxygen consumption throughout the growth and development of murine oocytes","volume":"76","author":"Harris","year":"2009","journal-title":"Mol Reprod Dev"},{"key":"2026030400563677800_iyag015-B28","doi-asserted-by":"publisher","first-page":"1230","DOI":"10.1002\/dvdy.70010","article-title":"Comprehensive observation of histone lysine lactylation during gametogenesis of Drosophila melanogaster","volume":"254","author":"Hayashi","year":"2025","journal-title":"Dev Dyn"},{"key":"2026030400563677800_iyag015-B29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/g3journal\/jkad228","article-title":"Metabolomic analysis of Drosophila melanogaster larvae lacking pyruvate kinase","volume":"14","author":"Heidarian","year":"2023","journal-title":"G3 (Bethesda)"},{"key":"2026030400563677800_iyag015-B30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13578-024-01240-6","article-title":"Multifunctional roles of \u03b3-enolase in the central nervous system: more than a neuronal marker","volume":"14","author":"Horvat","year":"2024","journal-title":"Cell Biosci"},{"key":"2026030400563677800_iyag015-B31","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1146\/annurev-physiol-021115-105045","article-title":"The biochemistry and physiology of mitochondrial fatty acid \u03b2-oxidation and its genetic disorders","volume":"78","author":"Houten","year":"2016","journal-title":"Annu Rev Physiol"},{"key":"2026030400563677800_iyag015-B32","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1146\/annurev-nutr-120420-025558","article-title":"Ins and outs of the TCA cycle: the central role of anaplerosis","volume":"41","author":"Inigo","year":"2021","journal-title":"Annu Rev Nutr"},{"key":"2026030400563677800_iyag015-B33","doi-asserted-by":"publisher","first-page":"1350","DOI":"10.1038\/nm.2882","article-title":"A PML-PPAR-\u03b4 pathway for fatty acid oxidation regulates hematopoietic stem cell maintenance","volume":"18","author":"Ito","year":"2012","journal-title":"Nat Med"},{"key":"2026030400563677800_iyag015-B34","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/j.stem.2024.01.003","article-title":"Metabolic regulation of the hallmarks of stem cell biology","volume":"31","author":"Jackson","year":"2024","journal-title":"Cell Stem Cell"},{"key":"2026030400563677800_iyag015-B35","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.expneurol.2018.07.009","article-title":"Glia-neuron energy metabolism in health and diseases: new insights into the role of nervous system metabolic transporters","volume":"309","author":"Jha","year":"2018","journal-title":"Exp Neurol"},{"key":"2026030400563677800_iyag015-B36","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1038\/s12276-023-00971-9","article-title":"Targeting glutamine metabolism as a therapeutic strategy for cancer","volume":"55","author":"Jin","year":"2023","journal-title":"Exp Mol Med"},{"key":"2026030400563677800_iyag015-B37","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1095\/biolreprod.106.059899","article-title":"Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo","volume":"77","author":"Johnson","year":"2007","journal-title":"Biol Reprod"},{"key":"2026030400563677800_iyag015-B38","doi-asserted-by":"publisher","first-page":"2637","DOI":"10.1101\/gad.287045.116","article-title":"Myc\/mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal","volume":"30","author":"Kanatsu-Shinohara","year":"2016","journal-title":"Genes Dev"},{"key":"2026030400563677800_iyag015-B39","doi-asserted-by":"publisher","first-page":"107906","DOI":"10.1016\/j.jbc.2024.107906","article-title":"Glycolysis: a multifaceted metabolic pathway and signaling hub","volume":"300","author":"Kierans","year":"2024","journal-title":"J Biol Chem"},{"key":"2026030400563677800_iyag015-B40","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/j.cmet.2020.08.011","article-title":"Glutamine metabolism controls stem cell fate reversibility and long-term maintenance in the hair follicle","volume":"32","author":"Kim","year":"2020","journal-title":"Cell Metab"},{"key":"2026030400563677800_iyag015-B41","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1016\/j.bbrc.2012.02.026","article-title":"Impaired fatty acid oxidation in a Drosophila model of mitochondrial trifunctional protein (MTP) deficiency","volume":"419","author":"Kishita","year":"2012","journal-title":"Biochem Biophys Res Commun"},{"key":"2026030400563677800_iyag015-B42","doi-asserted-by":"publisher","first-page":"128","DOI":"10.4161\/fly.4.2.11954","article-title":"Poly is required for nurse-cell chromosome dispersal and oocyte polarity in Drosophila","volume":"4","author":"Klusza","year":"2010","journal-title":"Fly (Austin)."},{"key":"2026030400563677800_iyag015-B43","doi-asserted-by":"publisher","first-page":"e79048","DOI":"10.1371\/journal.pone.0079048","article-title":"Prp22 and spliceosome components regulate chromatin dynamics in germ-line polyploid cells","volume":"8","author":"Klusza","year":"2013","journal-title":"PLoS One"},{"key":"2026030400563677800_iyag015-B44","doi-asserted-by":"publisher","first-page":"2144","DOI":"10.1016\/j.celrep.2017.08.029","article-title":"A fatty acid oxidation-dependent metabolic shift regulates adult neural stem cell activity","volume":"20","author":"Knobloch","year":"2017","journal-title":"Cell Rep"},{"key":"2026030400563677800_iyag015-B45","doi-asserted-by":"publisher","first-page":"1071","DOI":"10.1126\/science.1111410","article-title":"Direct control of germline stem cell division and cyst growth by neural insulin in Drosophila","volume":"309","author":"LaFever","year":"2005","journal-title":"Science"},{"key":"2026030400563677800_iyag015-B46","doi-asserted-by":"publisher","first-page":"2451","DOI":"10.1242\/dev.054882","article-title":"Specific roles of target of rapamycin in the control of stem cells and their progeny in the Drosophila ovary","volume":"137","author":"LaFever","year":"2010","journal-title":"Development"},{"key":"2026030400563677800_iyag015-B47","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1007\/978-1-4939-2851-4_4","article-title":"Genetic mosaic analysis of stem cell lineages in the Drosophila ovary","volume":"1328","author":"Laws","year":"2015","journal-title":"Methods Mol Biol"},{"key":"2026030400563677800_iyag015-B48","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.ydbio.2016.10.006","article-title":"AMP-activated protein kinase has diet-dependent and -independent roles in Drosophila oogenesis","volume":"420","author":"Laws","year":"2016","journal-title":"Dev Biol."},{"key":"2026030400563677800_iyag015-B49","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1016\/j.ydbio.2014.12.033","article-title":"Insulin-independent role of adiponectin receptor signaling in Drosophila germline stem cell maintenance","volume":"399","author":"Laws","year":"2015","journal-title":"Dev Biol"},{"key":"2026030400563677800_iyag015-B50","doi-asserted-by":"publisher","first-page":"112335","DOI":"10.1016\/j.celrep.2023.112335","article-title":"Neurons require glucose uptake and glycolysis in vivo","volume":"42","author":"Li","year":"2023","journal-title":"Cell Rep"},{"key":"2026030400563677800_iyag015-B51","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.aehs.2025.05.004","article-title":"Ketone bodies in exercise, health and disease: metabolic mechanisms, pathophysiology, and therapeutic implications","volume":"2","author":"Li","year":"2025","journal-title":"Adv Exerc Heal Sci"},{"key":"2026030400563677800_iyag015-B52","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1006\/dbio.1993.1228","article-title":"Germline stem cell division and egg chamber development in transplanted Drosophila germaria","volume":"159","author":"Lin","year":"1993","journal-title":"Dev Biol"},{"key":"2026030400563677800_iyag015-B53","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41392-025-02141-x","article-title":"Energy metabolism in health and diseases","volume":"10","author":"Liu","year":"2025","journal-title":"Signal Transduct Target Ther"},{"key":"2026030400563677800_iyag015-B54","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1016\/j.cmet.2017.08.024","article-title":"The Glia-neuron lactate shuttle and elevated ROS promote lipid synthesis in neurons and lipid droplet accumulation in Glia via APOE\/D","volume":"26","author":"Liu","year":"2017","journal-title":"Cell Metab"},{"key":"2026030400563677800_iyag015-B55","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1242\/dev.204384","article-title":"Histone modifications in development","volume":"152","author":"Liu","year":"2025","journal-title":"Development"},{"key":"2026030400563677800_iyag015-B56","doi-asserted-by":"publisher","first-page":"6807","DOI":"10.1038\/ncomms7807","article-title":"Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism","volume":"6","author":"Lundgaard","year":"2015","journal-title":"Nat Commun"},{"key":"2026030400563677800_iyag015-B57","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1242\/dev.00932","article-title":"Drosophila dMyc is required for ovary cell growth and endoreplication","volume":"131","author":"Maines","year":"2004","journal-title":"Development"},{"key":"2026030400563677800_iyag015-B58","doi-asserted-by":"publisher","first-page":"3797","DOI":"10.1242\/dev.121.11.3797","article-title":"Identification and behavior of epithelial stem cells in the Drosophila ovary","volume":"121","author":"Margolis","year":"1995","journal-title":"Development"},{"key":"2026030400563677800_iyag015-B59","doi-asserted-by":"publisher","first-page":"bio059286","DOI":"10.1242\/bio.059286","article-title":"Oo-site: a dashboard to visualize gene expression during Drosophila oogenesis suggests meiotic entry is regulated post-transcriptionally","volume":"11","author":"Martin","year":"2022","journal-title":"Biol Open"},{"key":"2026030400563677800_iyag015-B60","doi-asserted-by":"publisher","first-page":"e1010610","DOI":"10.1371\/journal.pgen.1010610","article-title":"Mitochondrial remodelling is essential for female germ cell differentiation and survival","volume":"19","author":"Monteiro","year":"2023","journal-title":"PLoS Genet"},{"key":"2026030400563677800_iyag015-B61","doi-asserted-by":"publisher","first-page":"2207","DOI":"10.1242\/dev.065508","article-title":"Long-term live imaging provides new insight into stem cell regulation and germline-soma coordination in the Drosophila ovary","volume":"138","author":"Morris","year":"2011","journal-title":"Development"},{"key":"2026030400563677800_iyag015-B62","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1016\/j.cell.2008.01.038","article-title":"Stem cells and niches: mechanisms that promote stem cell maintenance throughout life","volume":"132","author":"Morrison","year":"2008","journal-title":"Cell"},{"key":"2026030400563677800_iyag015-B63","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1016\/j.neuron.2011.05.011","article-title":"Integrating physiological regulation with stem cell and tissue homeostasis","volume":"70","author":"Nakada","year":"2011","journal-title":"Neuron"},{"key":"2026030400563677800_iyag015-B64","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/genetics\/iyad211","article-title":"FlyBase\u202f: updates to the Drosophila genes and genomes","volume":"227","author":"\u00d6zt\u00fcrk-\u00e7olak","year":"2024","journal-title":"Genetics"},{"key":"2026030400563677800_iyag015-B65","doi-asserted-by":"publisher","first-page":"1","DOI":"10.7554\/eLife.90509","article-title":"Chromatin and gene expression changes during female Drosophila germline stem cell development illuminate the biology of highly potent stem cells","volume":"12","author":"Pang","year":"2023","journal-title":"Elife"},{"key":"2026030400563677800_iyag015-B66","doi-asserted-by":"publisher","first-page":"eadd5220","DOI":"10.1126\/sciadv.add5220","article-title":"Mitochondrial pyruvate metabolism regulates the activation of quiescent adult neural stem cells","volume":"9","author":"Petrelli","year":"2023","journal-title":"Sci Adv"},{"key":"2026030400563677800_iyag015-B67","doi-asserted-by":"publisher","first-page":"852","DOI":"10.1038\/nmeth.3972","article-title":"Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs","volume":"13","author":"Port","year":"2016","journal-title":"Nat Methods"},{"key":"2026030400563677800_iyag015-B68","doi-asserted-by":"publisher","first-page":"19012","DOI":"10.1073\/pnas.1318481110","article-title":"Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9","volume":"110","author":"Ren","year":"2013","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2026030400563677800_iyag015-B69","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41467-024-52709-4","article-title":"piRNAs are regulators of metabolic reprogramming in stem cells","volume":"15","author":"Rojas-R\u00edos","year":"2024","journal-title":"Nat Commun"},{"key":"2026030400563677800_iyag015-B70","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1016\/j.stem.2015.11.012","article-title":"Metabolic reprogramming of stem cell epigenetics","volume":"17","author":"Ryall","year":"2015","journal-title":"Cell Stem Cell"},{"key":"2026030400563677800_iyag015-B71","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1038\/ncb3593","article-title":"Control of intestinal stem cell function and proliferation by mitochondrial pyruvate metabolism","volume":"19","author":"Schell","year":"2017","journal-title":"Nat Cell Biol"},{"key":"2026030400563677800_iyag015-B72","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1016\/j.cell.2015.12.020","article-title":"Electron transport chain remodeling by GSK3 during oogenesis connects nutrient state to reproduction","volume":"164","author":"Sieber","year":"2016","journal-title":"Cell"},{"key":"2026030400563677800_iyag015-B73","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1101\/gad.330464.119","article-title":"A single-cell atlas of the developing Drosophila ovary identifies follicle stem cell progenitors","volume":"34","author":"Slaidina","year":"2020","journal-title":"Genes Dev"},{"key":"2026030400563677800_iyag015-B74","doi-asserted-by":"publisher","first-page":"536","DOI":"10.1126\/science.303.5661.1138","article-title":"DCP-1, a Drosophila cell death protease essential for development","volume":"275","author":"Song","year":"1997","journal-title":"Science"},{"key":"2026030400563677800_iyag015-B75","first-page":"1","volume-title":"The Development of Drosophila melanogaster","author":"Spradling","year":"1993","edition":"1st ed"},{"key":"2026030400563677800_iyag015-B76","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/978-3-031-37936-9_1","article-title":"The ancient origin and function of germline cysts","volume":"71","author":"Spradling","year":"2024","journal-title":"Results Probl Cell Differ"},{"key":"2026030400563677800_iyag015-B77","doi-asserted-by":"publisher","first-page":"e58619","DOI":"10.7554\/elife.58619","article-title":"Gap junctions deliver malonyl-CoA from soma to germline to support embryogenesis in Caenorhabditis elegans","volume":"9","author":"Starich","year":"2020","journal-title":"eLife"},{"key":"2026030400563677800_iyag015-B78","doi-asserted-by":"publisher","first-page":"2306","DOI":"10.1002\/stem.2042","article-title":"Neural stem cells in the adult subventricular zone oxidize fatty acids to produce energy and support neurogenic activity","volume":"33","author":"Stoll","year":"2015","journal-title":"Stem Cells"},{"key":"2026030400563677800_iyag015-B79","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1139\/G08-023","article-title":"Mutations of the withered (whd) gene in Drosophila melanogaster confer hypersensitivity to oxidative stress and are lesions of the carnitine palmitoyltransferase I (CPT I) gene","volume":"51","author":"Strub","year":"2008","journal-title":"Genome"},{"key":"2026030400563677800_iyag015-B80","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.stem.2012.10.011","article-title":"Regulation of glycolysis by pdk functions as a metabolic checkpoint for cell cycle quiescence in hematopoietic stem cells","volume":"12","author":"Takubo","year":"2013","journal-title":"Cell Stem Cell"},{"key":"2026030400563677800_iyag015-B81","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1038\/ncb3165","article-title":"ATP synthase promotes germ cell differentiation independent of oxidative phosphorylation","volume":"17","author":"Teixeira","year":"2015","journal-title":"Nat Cell Biol"},{"key":"2026030400563677800_iyag015-B82","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.cmet.2011.01.005","article-title":"The Drosophila estrogen-related receptor directs a metabolic switch that supports developmental growth","volume":"13","author":"Tennessen","year":"2011","journal-title":"Cell Metab"},{"key":"2026030400563677800_iyag015-B83","doi-asserted-by":"publisher","first-page":"1","DOI":"10.7554\/eLife.53247","article-title":"Fatty acid \u03b2-oxidation is required for the differentiation of larval hematopoietic progenitors in Drosophila","volume":"9","author":"Tiwari","year":"2020","journal-title":"Elife"},{"key":"2026030400563677800_iyag015-B84","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fcell.2020.00087","article-title":"Energy metabolism regulates stem cell pluripotency","volume":"8","author":"Tsogtbaatar","year":"2020","journal-title":"Front Cell Dev Biol"},{"key":"2026030400563677800_iyag015-B85","doi-asserted-by":"publisher","first-page":"e3003045","DOI":"10.1371\/journal.pbio.3003045","article-title":"Gap junctions allow transfer of metabolites between germ cells and somatic cells to promote germ cell growth in the Drosophila ovary","volume":"23","author":"Vachias","year":"2025","journal-title":"PLoS Biol"},{"key":"2026030400563677800_iyag015-B86","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1038\/nmeth.1331","article-title":"Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster","volume":"6","author":"Venken","year":"2009","journal-title":"Nat Methods"},{"key":"2026030400563677800_iyag015-B87","doi-asserted-by":"publisher","first-page":"3697","DOI":"10.1242\/dev.025114","article-title":"\u03b1-Endosulfine is a conserved protein required for oocyte meiotic maturation in Drosophila","volume":"135","author":"Von Stetina","year":"2008","journal-title":"Development"},{"key":"2026030400563677800_iyag015-B88","doi-asserted-by":"publisher","first-page":"2813","DOI":"10.1002\/dvdy.22418","article-title":"Drosophila variable nurse cells encodes arrest defective 1 (ARD1), the catalytic subunit of the major N-terminal acetyltransferase complex","volume":"239","author":"Wang","year":"2010","journal-title":"Dev Dyn"},{"key":"2026030400563677800_iyag015-B89","doi-asserted-by":"publisher","first-page":"2249","DOI":"10.1016\/j.devcel.2023.08.007","article-title":"Mechanical stimulation from the surrounding tissue activates mitochondrial energy metabolism in Drosophila differentiating germ cells","volume":"58","author":"Wang","year":"2023","journal-title":"Dev Cell"},{"key":"2026030400563677800_iyag015-B90","doi-asserted-by":"publisher","first-page":"1","DOI":"10.7554\/eLife.49309","article-title":"Electron transport chain biogenesis activated by a JNK-insulin-MYC relay primes mitochondrial inheritance in Drosophila","volume":"8","author":"Wang","year":"2019","journal-title":"Elife"},{"key":"2026030400563677800_iyag015-B91","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/B978-0-323-95424-2.00053-4","volume-title":"Comprehensive Molecular Insect Science","author":"Wessel","year":"2026","edition":"2nd ed"},{"key":"2026030400563677800_iyag015-B92","doi-asserted-by":"publisher","first-page":"7477","DOI":"10.1523\/JNEUROSCI.0415-11.2011","article-title":"In vivo evidence for lactate as a neuronal energy source","volume":"31","author":"Wyss","year":"2011","journal-title":"J Neurosci"},{"key":"2026030400563677800_iyag015-B93","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.ydbio.2010.08.018","article-title":"Categorical data analysis in experimental biology","volume":"348","author":"Xu","year":"2010","journal-title":"Dev Biol"},{"key":"2026030400563677800_iyag015-B94","doi-asserted-by":"publisher","first-page":"1223","DOI":"10.1242\/dev.117.4.1223","article-title":"Analysis of genetic mosaics in developing and adult Drosophila tissues","volume":"117","author":"Xu","year":"1993","journal-title":"Development"},{"key":"2026030400563677800_iyag015-B95","doi-asserted-by":"publisher","first-page":"4821","DOI":"10.3390\/ijms25094821","article-title":"Histone lactylation is involved in mouse oocyte maturation and embryo development","volume":"25","author":"Yang","year":"2024","journal-title":"Int J Mol Sci"},{"key":"2026030400563677800_iyag015-B96","doi-asserted-by":"publisher","first-page":"966","DOI":"10.1016\/j.cmet.2019.01.016","article-title":"Glutamine metabolism regulates proliferation and lineage allocation in skeletal stem cells","volume":"29","author":"Yu","year":"2019","journal-title":"Cell Metab"},{"key":"2026030400563677800_iyag015-B97","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s13577-025-01172-4","article-title":"The multifaceted roles of aldolase A in cancer: glycolysis, cytoskeleton, translation and beyond","volume":"38","author":"Yu","year":"2025","journal-title":"Hum Cell"},{"key":"2026030400563677800_iyag015-B98","doi-asserted-by":"publisher","first-page":"3704","DOI":"10.1016\/j.cub.2022.07.003","article-title":"Article EGFR signaling activates intestinal stem cells by promoting mitochondrial biogenesis and \u03b2-oxidation EGFR signaling activates intestinal stem cells by promoting mitochondrial biogenesis and \u03b2-oxidation","volume":"32","author":"Zhang","year":"2022","journal-title":"Curr Biol"},{"key":"2026030400563677800_iyag015-B99","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.ydbio.2025.05.005","article-title":"Estrogen-related receptor is required in adult Drosophila females for germline stem cell maintenance","volume":"524","author":"Zike","year":"2025","journal-title":"Dev Biol"},{"key":"2026030400563677800_iyag015-B100","doi-asserted-by":"publisher","first-page":"315","DOI":"10.3390\/metabo12040315","article-title":"The microRNA miR-277 controls physiology and pathology of the adult Drosophila midgut by regulating the expression of fatty acid \u03b2-oxidation-related genes in intestinal stem cells","volume":"12","author":"Zipper","year":"2022","journal-title":"Metabolites"}],"container-title":["GENETICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/genetics\/advance-article-pdf\/doi\/10.1093\/genetics\/iyag015\/66483233\/iyag015.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/genetics\/article-pdf\/232\/3\/iyag015\/66483233\/iyag015.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/genetics\/article-pdf\/232\/3\/iyag015\/66483233\/iyag015.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T05:56:47Z","timestamp":1772603807000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/genetics\/article\/doi\/10.1093\/genetics\/iyag015\/8431687"}},"subtitle":[],"editor":[{"given":"D","family":"Greenstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1,20]]},"references-count":100,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,1,20]]},"published-print":{"date-parts":[[2026,3,4]]}},"URL":"https:\/\/doi.org\/10.1093\/genetics\/iyag015","relation":{},"ISSN":["1943-2631"],"issn-type":[{"value":"1943-2631","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,3]]},"published":{"date-parts":[[2026,1,20]]},"article-number":"iyag015"}}