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Whether activated Rho and Phospholipase C signaling acts through shared or independent pathways remains unclear. We use the egg-laying circuit of the nematode worm, Caenorhabditis elegans, which is regulated positively by G\u03b1q signaling and negatively by G\u03b1o signaling, to determine the role of Rho GTPase in G-protein regulation of neural circuit excitability. We previously showed that Trio signals in both the presynaptic hermaphrodite-specific command neurons (HSNs) and the postsynaptic egg-laying vulval muscles they innervate to promote egg laying. Here, we show that its effector Rho similarly signals both presynaptically and postsynaptically. Expression of constitutively active Rho-1(G14V) in either the HSNs or vulval muscles increased their Ca2+ activity and stimulated egg laying. Conversely, reducing Rho signaling inhibited HSN Ca2+ activity and egg laying. Animals lacking Rho function in HSN still had vulval muscle Ca2+ activity that supported contraction and egg release, although this Ca2+ activity lacked the enhancement associated with HSN neurotransmitter release, suggesting it was largely driven by stretch-dependent feedback. Together, these results show that Rho signals in both the HSNs and vulval muscles promote excitability and egg laying.<\/jats:p>","DOI":"10.1093\/genetics\/iyag046","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T12:41:33Z","timestamp":1770986493000},"source":"Crossref","is-referenced-by-count":0,"title":["Rho signaling promotes cell excitability and neurotransmission"],"prefix":"10.1093","volume":"232","author":[{"given":"Ariana","family":"Jos\u00e9","sequence":"first","affiliation":[{"name":"Program in Cellular Physiology and Molecular Biophysics, Miller School of Medicine, University of Miami , Miami, FL 33136 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pravat","family":"Dhakal","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Miami , 1301 Memorial Drive, Coral Gables, FL 33146 ,","place":["United 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