{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T02:55:44Z","timestamp":1789786544246,"version":"4.0.1"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012264","name":"NIH","doi-asserted-by":"publisher","award":["AI152494"],"award-info":[{"award-number":["AI152494"]}],"id":[{"id":"10.13039\/501100012264","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"TRR 124 FungiNet","award":["210879364"],"award-info":[{"award-number":["210879364"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The biological conservation between fungi and mammals due to a common ancestor has made development of selective antifungal drugs a difficult challenge. Further complicating this situation is the selection of antifungal drug-resistant organisms during drug treatment. The pathogenic yeast Nakaseomyces glabratus (called here Candida glabrata) presents an especially challenging organism due to its tendency to frequently lose susceptibility to the major antifungal drug class the azoles. Additionally, C. glabrata develops resistance to echinocandin drugs, a second, more recently described antifungal agent at 10 times the rate of other organisms. Previous work has established that the sterol-responsive transcriptional regulator Upc2A is a key determinant of azole susceptibility in C. glabrata and plays a role in echinocandin resistance. We used a biochemical approach to identify proteins that co-purified with Upc2A and identified the Ypk2 AGC kinase as an interacting protein. Strains lacking YPK2 exhibited increased susceptibility to fluconazole and the echinocandin caspofungin. A ypk2\u0394 strain failed to normally induce transcription of several ERG genes but exhibited normal induction of the CDR1 ATP-binding cassette transporter gene. Isogenic ypk2\u0394 strains were also highly susceptible to the 3 major classes of antifungal drugs, indicating that this kinase behaves as a multidrug susceptibility factor. RNA-seq analyses indicated that the transcriptional response to exposure is different for each drug, and each response is differentially altered upon loss of Ypk2. Our data indicate that Ypk2 plays an important role in coordinating gene expression that impacts susceptibility to all major antifungal drug classes.<\/jats:p>","DOI":"10.1093\/genetics\/iyag176","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T13:02:50Z","timestamp":1783688570000},"source":"Crossref","is-referenced-by-count":1,"title":["<i>Candida glabrata YPK2<\/i>\n                    is a multidrug susceptibility locus"],"prefix":"10.1093","volume":"234","author":[{"given":"Lucia","family":"Simonicova","sequence":"first","affiliation":[{"name":"Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa , Iowa City, IA 52242 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas P","family":"Conway","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa , Iowa City, IA 52242 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Kr\u00fcger","sequence":"additional","affiliation":[{"name":"Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Kn\u00f6ll Institute Jena , Jena 07745 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel A","family":"Brakhage","sequence":"additional","affiliation":[{"name":"Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Kn\u00f6ll Institute Jena , Jena 07745 ,","place":["Germany"]},{"name":"Institute of Microbiology, Friedrich Schiller University Jena , Jena 07743 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7163-1120","authenticated-orcid":false,"given":"W Scott","family":"Moye-Rowley","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa , Iowa City, IA 52242 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,10]]},"reference":[{"key":"2026090204121516600_iyag176-B1","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1038\/nchembio.1496","article-title":"Amphotericin forms an extramembranous and fungicidal sterol sponge","volume":"10","author":"Anderson","year":"2014","journal-title":"Nat Chem Biol"},{"key":"2026090204121516600_iyag176-B2","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1083\/jcb.200409045","article-title":"Dynamic ergosterol- and ceramide-rich domains in the peroxisomal membrane serve as an organizing platform for peroxisome fusion","volume":"168","author":"Boukh-Viner","year":"2005","journal-title":"J Cell Biol"},{"key":"2026090204121516600_iyag176-B3","doi-asserted-by":"publisher","first-page":"1048","DOI":"10.1038\/nature08787","article-title":"Orm family proteins mediate sphingolipid homeostasis","volume":"463","author":"Breslow","year":"2010","journal-title":"Nature"},{"key":"2026090204121516600_iyag176-B4","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1146\/annurev.cellbio.14.1.111","article-title":"Functions of lipid rafts in biological membranes","volume":"14","author":"Brown","year":"1998","journal-title":"Annu Rev Cell Dev Biol"},{"key":"2026090204121516600_iyag176-B5","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.834411","article-title":"Determinants of peroxisome membrane dynamics","volume":"13","author":"Carmichael","year":"2022","journal-title":"Front Physiol"},{"key":"2026090204121516600_iyag176-B6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004487","article-title":"ChIP-seq and in vivo transcriptome analyses of the Aspergillus fumigatus SREBP SrbA reveals a new regulator of the fungal hypoxia response and virulence","volume":"10","author":"Chung","year":"2014","journal-title":"PLoS Pathog"},{"key":"2026090204121516600_iyag176-B7","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00792-24","article-title":"Similarities and distinctions in the activation of the Candida glabrata Pdr1 regulatory pathway by azole and non-azole drugs","volume":"9","author":"Conway","year":"2024","journal-title":"mSphere"},{"key":"2026090204121516600_iyag176-B8","doi-asserted-by":"publisher","first-page":"4177","DOI":"10.1128\/JB.180.16.4177-4183.1998","article-title":"A mutation in a purported regulatory gene affects control of sterol uptake in Saccharomyces cerevisiae","volume":"180","author":"Crowley","year":"1998","journal-title":"J Bacteriol"},{"key":"2026090204121516600_iyag176-B9","doi-asserted-by":"publisher","DOI":"10.3390\/jof11060465","article-title":"Species distribution and antifungal susceptibility patterns of invasive candidiasis in a Belgian tertiary center: a 7-year retrospective analysis","volume":"11","author":"Cugnata","year":"2025","journal-title":"J Fungi (Basel)"},{"key":"2026090204121516600_iyag176-B10","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aad4016","article-title":"Revisiting protein kinase-substrate interactions: toward therapeutic development","volume":"9","author":"de Oliveira","year":"2016","journal-title":"Sci Signal"},{"key":"2026090204121516600_iyag176-B11","doi-asserted-by":"publisher","first-page":"1142","DOI":"10.1016\/S0140-6736(03)14472-8","article-title":"Echinocandin antifungal drugs","volume":"362","author":"Denning","year":"2003","journal-title":"Lancet"},{"key":"2026090204121516600_iyag176-B12","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1093\/nar\/30.1.207","article-title":"Gene Expression Omnibus: NCBI gene expression and hybridization array data repository","volume":"30","author":"Edgar","year":"2002","journal-title":"Nucleic Acids Res"},{"key":"2026090204121516600_iyag176-B13","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1146\/annurev-cellbio-011723-030346","article-title":"Regulation of TORC2 function and localization in yeast","volume":"39","author":"Emmerstorfer-Augustin","year":"2023","journal-title":"Annu Rev Cell Dev Biol"},{"key":"2026090204121516600_iyag176-B14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000268","article-title":"Gain of function mutations in CgPDR1 of Candida glabrata not only mediate antifungal resistance but also enhance virulence","volume":"5","author":"Ferrari","year":"2009","journal-title":"PLoS Pathog"},{"key":"2026090204121516600_iyag176-B15","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1038\/s41579-022-00720-1","article-title":"Tackling the emerging threat of antifungal resistance to human health","volume":"20","author":"Fisher","year":"2022","journal-title":"Nat Rev Microbiol"},{"key":"2026090204121516600_iyag176-B16","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/S0076-6879(02)50957-5","article-title":"Transformation of yeast by lithium acetate\/single-stranded carrier DNA\/polyethylene glycol method","volume":"350","author":"Gietz","year":"2002","journal-title":"Methods Enzymol"},{"key":"2026090204121516600_iyag176-B17","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1128\/mSphere.00218-17","article-title":"Use of RNA-protein complexes for genome editing in non-albicans Candida species","volume":"2","author":"Grahl","year":"2017","journal-title":"mSphere"},{"key":"2026090204121516600_iyag176-B18","doi-asserted-by":"publisher","first-page":"3687","DOI":"10.1002\/j.1460-2075.1994.tb06678.x","article-title":"Ceramide synthesis enhances transport of GPI-anchored proteins to the Golgi apparatus in yeast","volume":"13","author":"Horvath","year":"1994","journal-title":"EMBO J"},{"key":"2026090204121516600_iyag176-B19","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1042\/BJ20071518","article-title":"TOR regulation of AGC kinases in yeast and mammals","volume":"410","author":"Jacinto","year":"2008","journal-title":"Biochem J"},{"key":"2026090204121516600_iyag176-B20","doi-asserted-by":"publisher","first-page":"7239","DOI":"10.1128\/MCB.25.16.7239-7248.2005","article-title":"Tor2 directly phosphorylates the AGC kinase Ypk2 to regulate actin polarization","volume":"25","author":"Kamada","year":"2005","journal-title":"Mol Cell Biol"},{"key":"2026090204121516600_iyag176-B21","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.00021-23","article-title":"Candida albicans and Candida glabrata: global priority pathogens","volume":"88","author":"Katsipoulaki","year":"2024","journal-title":"Microbiol Mol Biol Rev"},{"key":"2026090204121516600_iyag176-B22","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1111\/mmi.13913","article-title":"Positive autoregulation and repression of transactivation are key regulatory features of the Candida glabrata Pdr1 transcription factor","volume":"107","author":"Khakhina","year":"2018","journal-title":"Mol Microbiol"},{"key":"2026090204121516600_iyag176-B23","doi-asserted-by":"publisher","first-page":"99","DOI":"10.5941\/MYCO.2016.44.2.99","article-title":"Isolation frequency characteristics of Candida species from clinical specimens","volume":"44","author":"Kim","year":"2016","journal-title":"Mycobiology"},{"key":"2026090204121516600_iyag176-B24","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1128\/EC.2.2.284-294.2003","article-title":"Ceramide\/long-chain base phosphate rheostat in Saccharomyces cerevisiae: regulation of ceramide synthesis by Elo3p and Cka2p","volume":"2","author":"Kobayashi","year":"2003","journal-title":"Eukaryot Cell"},{"key":"2026090204121516600_iyag176-B25","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13093","article-title":"Variations in yeast plasma-membrane lipid composition affect killing activity of three families of insect antifungal peptides","volume":"21","author":"Kodedova","year":"2019","journal-title":"Cell Microbiol"},{"key":"2026090204121516600_iyag176-B26","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1534\/genetics.167.1.35","article-title":"Analysis of beta-1,3-glucan assembly in Saccharomyces cerevisiae using a synthetic interaction network and altered sensitivity to caspofungin","volume":"167","author":"Lesage","year":"2004","journal-title":"Genetics"},{"key":"2026090204121516600_iyag176-B27","doi-asserted-by":"publisher","first-page":"5393","DOI":"10.1128\/MCB.19.8.5393","article-title":"Transcriptional elements involved in the repression of ribosomal protein synthesis","volume":"19","author":"Li","year":"1999","journal-title":"Mol Cell Biol"},{"key":"2026090204121516600_iyag176-B28","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2026090204121516600_iyag176-B29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0230246","article-title":"Multi-kinase control of environmental stress responsive transcription","volume":"15","author":"Mace","year":"2020","journal-title":"PLoS One"},{"key":"2026090204121516600_iyag176-B30","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1083\/jcb.201502033","article-title":"Ltc1 is an ER-localized sterol transporter and a component of ER-mitochondria and ER-vacuole contacts","volume":"209","author":"Murley","year":"2015","journal-title":"J Cell Biol"},{"key":"2026090204121516600_iyag176-B31","doi-asserted-by":"publisher","first-page":"2679","DOI":"10.1083\/jcb.201610032","article-title":"Sterol transporters at membrane contact sites regulate TORC1 and TORC2 signaling","volume":"216","author":"Murley","year":"2017","journal-title":"J Cell Biol"},{"key":"2026090204121516600_iyag176-B32","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1111\/j.1365-2443.2010.01470.x","article-title":"Transcription factors CgUPC2A and CgUPC2B regulate ergosterol biosynthetic genes in Candida glabrata","volume":"16","author":"Nagi","year":"2011","journal-title":"Genes Cells"},{"key":"2026090204121516600_iyag176-B33","doi-asserted-by":"publisher","first-page":"3037","DOI":"10.1128\/AAC.45.11.3037-3045.2001","article-title":"In vitro and in vivo effects of 14alpha-demethylase (ERG11) depletion in Candida glabrata","volume":"45","author":"Nakayama","year":"2001","journal-title":"Antimicrob Agents Chemother"},{"key":"2026090204121516600_iyag176-B34","doi-asserted-by":"publisher","first-page":"722","DOI":"10.1038\/s42003-024-06411-2","article-title":"Activation of the cell wall integrity pathway negatively regulates TORC2-Ypk1\/2 signaling through blocking eisosome disassembly in Saccharomyces cerevisiae","volume":"7","author":"Nomura","year":"2024","journal-title":"Commun Biol"},{"key":"2026090204121516600_iyag176-B35","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00830-21","article-title":"Loss-of-function ROX1 mutations suppress the fluconazole susceptibility of upc2ADelta mutation in Candida glabrata, implicating additional positive regulators of ergosterol biosynthesis","volume":"6","author":"Ollinger","year":"2021","journal-title":"mSphere"},{"key":"2026090204121516600_iyag176-B36","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1093\/cid\/civ1194","article-title":"Executive summary: clinical practice guideline for the management of candidiasis: 2016 update by the Infectious Diseases Society of America","volume":"62","author":"Pappas","year":"2016","journal-title":"Clin Infect Dis"},{"key":"2026090204121516600_iyag176-B37","doi-asserted-by":"publisher","first-page":"694","DOI":"10.1128\/AAC.00861-07","article-title":"Multidrug transporters CaCdr1p and CaMdr1p of Candida albicans display different lipid specificities: both ergosterol and sphingolipids are essential for targeting of CaCdr1p to membrane rafts","volume":"52","author":"Pasrija","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"key":"2026090204121516600_iyag176-B38","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1111\/mmi.14109","article-title":"Negative regulation of Candida glabrata Pdr1 by the deubiquitinase subunit Bre5 occurs in a ubiquitin independent manner","volume":"110","author":"Paul","year":"2018","journal-title":"Mol Microbiol"},{"key":"2026090204121516600_iyag176-B39","doi-asserted-by":"publisher","first-page":"D543","DOI":"10.1093\/nar\/gkae1011","article-title":"The PRIDE database at 20 years: 2025 update","volume":"53","author":"Perez-Riverol","year":"2025","journal-title":"Nucleic Acids Res"},{"key":"2026090204121516600_iyag176-B40","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1146\/annurev-micro-092611-150111","article-title":"Yeast ATP-binding cassette transporters conferring multidrug resistance","volume":"66","author":"Prasad","year":"2012","journal-title":"Annu Rev Microbiol"},{"key":"2026090204121516600_iyag176-B41","doi-asserted-by":"publisher","first-page":"1030","DOI":"10.1038\/13732","article-title":"A generic protein purification method for protein complex characterization and proteome exploration","volume":"17","author":"Rigaut","year":"1999","journal-title":"Nat Biotechnol"},{"key":"2026090204121516600_iyag176-B42","doi-asserted-by":"publisher","first-page":"19222","DOI":"10.1073\/pnas.1116948108","article-title":"Protein kinase Ypk1 phosphorylates regulatory proteins Orm1 and Orm2 to control sphingolipid homeostasis in Saccharomyces cerevisiae","volume":"108","author":"Roelants","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026090204121516600_iyag176-B43","doi-asserted-by":"publisher","DOI":"10.3390\/biom7030066","article-title":"The TORC2-dependent signaling network in the yeast Saccharomyces cerevisiae","volume":"7","author":"Roelants","year":"2017","journal-title":"Biomolecules"},{"key":"2026090204121516600_iyag176-B44","doi-asserted-by":"publisher","first-page":"3005","DOI":"10.1091\/mbc.e02-04-0201","article-title":"Pkh1 and pkh2 differentially phosphorylate and activate ypk1 and ykr2 and define protein kinase modules required for maintenance of cell wall integrity","volume":"13","author":"Roelants","year":"2002","journal-title":"Mol Biol Cell"},{"key":"2026090204121516600_iyag176-B45","doi-asserted-by":"publisher","first-page":"2128","DOI":"10.1091\/mbc.E18-04-0229","article-title":"TOR complex 2-regulated protein kinase Ypk1 controls sterol distribution by inhibiting StARkin domain-containing proteins located at plasma membrane-endoplasmic reticulum contact sites","volume":"29","author":"Roelants","year":"2018","journal-title":"Mol Biol Cell"},{"key":"2026090204121516600_iyag176-B46","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1083\/jcb.146.4.741","article-title":"Electrospray ionization tandem mass spectrometry (ESI-MS\/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting\/remodeling of distinct molecular species en route to the plasma membrane","volume":"146","author":"Schneiter","year":"1999","journal-title":"J Cell Biol"},{"key":"2026090204121516600_iyag176-B47","doi-asserted-by":"publisher","first-page":"2469","DOI":"10.1091\/mbc.e09-10-0899","article-title":"Differential roles of transcriptional mediator subunits in regulation of multidrug resistance gene expression in Saccharomyces cerevisiae","volume":"21","author":"Shahi","year":"2010","journal-title":"Mol Biol Cell"},{"key":"2026090204121516600_iyag176-B48","doi-asserted-by":"publisher","first-page":"1589","DOI":"10.1042\/BST20201136","article-title":"Transcriptional control of ribosome biogenesis in yeast: links to growth and stress signals","volume":"49","author":"Shore","year":"2021","journal-title":"Biochem Soc Trans"},{"key":"2026090204121516600_iyag176-B49","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1009005","article-title":"Functional information from clinically-derived drug resistant forms of the Candida glabrata Pdr1 transcription factor","volume":"16","author":"Simonicova","year":"2020","journal-title":"PLoS Genet"},{"key":"2026090204121516600_iyag176-B50","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a004697","article-title":"Membrane organization and lipid rafts","volume":"3","author":"Simons","year":"2011","journal-title":"Cold Spring Harb Perspect Biol"},{"key":"2026090204121516600_iyag176-B51","doi-asserted-by":"publisher","first-page":"10503","DOI":"10.1038\/s41598-019-46385-4","article-title":"Large-scale discovery of substrates of the human kinome","volume":"9","author":"Sugiyama","year":"2019","journal-title":"Sci Rep"},{"key":"2026090204121516600_iyag176-B52","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.1038\/s41589-022-01117-0","article-title":"Structural basis for activation of fungal sterol receptor Upc2 and azole resistance","volume":"18","author":"Tan","year":"2022","journal-title":"Nat Chem Biol"},{"key":"2026090204121516600_iyag176-B53","doi-asserted-by":"publisher","first-page":"1384","DOI":"10.1128\/AAC.50.4.1384-1392.2006","article-title":"Candida glabrata PDR1, a transcriptional regulator of a pleiotropic drug resistance network, mediates azole resistance in clinical isolates and petite mutants","volume":"50","author":"Tsai","year":"2006","journal-title":"Antimicrob Agents Chemother"},{"key":"2026090204121516600_iyag176-B54","doi-asserted-by":"publisher","first-page":"704","DOI":"10.1111\/j.1365-2958.2006.05235.x","article-title":"Pdr1 regulates multidrug resistance in Candida glabrata: gene disruption and genome-wide expression studies","volume":"61","author":"Vermitsky","year":"2006","journal-title":"Mol Microbiol"},{"key":"2026090204121516600_iyag176-B55","doi-asserted-by":"publisher","first-page":"3773","DOI":"10.1128\/AAC.48.10.3773-3781.2004","article-title":"Azole resistance in Candida glabrata: coordinate upregulation of multidrug transporters and evidence for a Pdr1-like transcription factor","volume":"48","author":"Vermitsky","year":"2004","journal-title":"Antimicrob Agents Chemother"},{"key":"2026090204121516600_iyag176-B56","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1128\/mSphere.00099-18","article-title":"Construction and use of a recyclable marker to examine the role of major facilitator superfamily protein members in Candida glabrata drug resistance phenotypes","volume":"3","author":"Vu","year":"2018","journal-title":"mSphere"},{"key":"2026090204121516600_iyag176-B57","doi-asserted-by":"publisher","DOI":"10.1128\/aac.02098-21","article-title":"Azole-resistant alleles of ERG11 in Candida glabrata trigger activation of the Pdr1 and Upc2A transcription factors","volume":"66","author":"Vu","year":"2022","journal-title":"Antimicrob Agents Chemother"},{"key":"2026090204121516600_iyag176-B58","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1009582","article-title":"The Candida glabrata Upc2A transcription factor is a global regulator of antifungal drug resistance pathways","volume":"17","author":"Vu","year":"2021","journal-title":"PLoS Genet"},{"key":"2026090204121516600_iyag176-B59","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1128\/mBio.00934-19","article-title":"Evidence that ergosterol biosynthesis modulates activity of the Pdr1 transcription factor in Candida glabrata","volume":"10","author":"Vu","year":"2019","journal-title":"MBio"},{"key":"2026090204121516600_iyag176-B60","doi-asserted-by":"publisher","first-page":"6129","DOI":"10.1038\/ncomms7129","article-title":"Structural mechanism of ergosterol regulation by fungal sterol transcription factor Upc2","volume":"6","author":"Yang","year":"2015","journal-title":"Nat Commun"},{"key":"2026090204121516600_iyag176-B61","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/0378-1119(85)90120-9","article-title":"Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors","volume":"33","author":"Yanisch-Perron","year":"1985","journal-title":"Gene"}],"container-title":["GENETICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/genetics\/advance-article-pdf\/doi\/10.1093\/genetics\/iyag176\/68754738\/iyag176.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/genetics\/article-pdf\/234\/1\/iyag176\/68754738\/iyag176.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/genetics\/article-pdf\/234\/1\/iyag176\/68754738\/iyag176.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T08:14:05Z","timestamp":1788336845000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/genetics\/article\/doi\/10.1093\/genetics\/iyag176\/8731919"}},"subtitle":[],"editor":[{"given":"L","family":"Rusche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,7,10]]},"references-count":61,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,7,10]]},"published-print":{"date-parts":[[2026,9,2]]}},"URL":"https:\/\/doi.org\/10.1093\/genetics\/iyag176","relation":{},"ISSN":["1943-2631"],"issn-type":[{"value":"1943-2631","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9]]},"published":{"date-parts":[[2026,7,10]]},"article-number":"iyag176"}}