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We identify two sperm-specific PDZ proteins, SMZ-1 and SMZ-2 (SMZ-1\/2), that initiate MSP loading into preformed membranous organelles in Caenorhabditis elegans. SMZ-1\/2 were expressed specifically in spermatogenic germ cells, colocalized with developing FB-MOs from diplotene through spermatid formation, and were redundant for fertility. Loss of SMZ-1\/2 abolished MSP filaments, left MOs lacking MSP filaments that are morphologically abnormal, and caused primary spermatocyte arrest. Electron microscopy showed fewer, smaller FB-MOs lacking crystalline FBs, consistent with a defect in assembly initiation rather than late polymerization. SMZ-1\/2 cytoplasmic structures were absent in spe-6 casein kinase mutants, placing SMZ-1\/2 within a SPE-6-dependent initiation module. These findings identify SMZ-1\/2 as PDZ scaffold proteins that initiate MSP assembly and couple kinase signaling to FB-MO biogenesis during nematode spermatogenesis.<\/jats:p>","DOI":"10.1093\/genetics\/iyag212","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T13:02:45Z","timestamp":1788181365000},"source":"Crossref","is-referenced-by-count":1,"title":["Sperm-specific fertility factors SMZ-1\/2 promote FB-MO biogenesis and MSP filament assembly in\n                    <i>C. elegans<\/i>"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1938-2913","authenticated-orcid":false,"given":"Hsiao-Fang","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Science and Medical Biotechnology, College of Medicine, National Taiwan University , Taipei 100229 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