{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T12:59:33Z","timestamp":1791377973609,"version":"4.3.2"},"reference-count":99,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100012264","name":"NIH","doi-asserted-by":"publisher","award":["NINDS-R21NS114774"],"award-info":[{"award-number":["NINDS-R21NS114774"]}],"id":[{"id":"10.13039\/501100012264","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020414","name":"NSF","doi-asserted-by":"publisher","award":["HRD-1736019"],"award-info":[{"award-number":["HRD-1736019"]}],"id":[{"id":"10.13039\/501100020414","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NIH-NIGMS","award":["R25GM061838"],"award-info":[{"award-number":["R25GM061838"]}]},{"name":"NIH-NIGMS","award":["R25GM061151-21"],"award-info":[{"award-number":["R25GM061151-21"]}]},{"name":"NIH NIGMS","award":["COBRE P30GM149367"],"award-info":[{"award-number":["COBRE P30GM149367"]}]},{"name":"NIH-RCMI","award":["5U54MD007600"],"award-info":[{"award-number":["5U54MD007600"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>There is clear evidence that Earth's temperature is rising at an unprecedented rate. While consequences on ecosystems are being extensively studied, little is known about the consequences of temperature on the nervous system of ectothermic animals. Here, we used the Drosophila larval NMJ to ask whether phasic 1s and tonic 1b motor neuron terminals differ in their structural response to rearing temperature. We find that the tonic 1b terminal's bouton number is not affected by temperature; however, we do observe a temperature-dependent synaptic growth in the phasic neuron, which might be related to the increased motility observed previously at higher temperatures. We find that the level of autophagy activity changes with temperature and that autophagy genes are responsible for the temperature dependence of synaptic growth. We present evidence that this regulation could occur through the major synaptic growth regulator and ubiquitin ligase Highwire and a pathway involving the mitogen-activated protein kinases. We present a new function for the MAPKKK, Wallenda, and the MAPK P38b in directing the additional synaptic growth that takes place between 25 and 29 \u00b0C. This illustrates that temperature has different effects on a diverse population of neurons and that distinct genetic pathways are involved in regulating temperature-driven changes.<\/jats:p>","DOI":"10.1093\/genetics\/iyag226","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T10:39:51Z","timestamp":1787654391000},"source":"Crossref","is-referenced-by-count":0,"title":["Autophagy, the ubiquitin proteasome system, and the MAPK pathway control the temperature dependence of synaptic growth"],"prefix":"10.1093","volume":"234","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1315-7798","authenticated-orcid":false,"given":"Kevin M","family":"De Leon Gonzalez","sequence":"first","affiliation":[{"name":"Institute of Neurobiology, Medical Sciences Campus, University of Puerto Rico , San Juan, Puerto Rico 00901 ,","place":["United States"]},{"name":"Department of Biology, University of Puerto Rico, Rio Piedras , San Juan, Puerto Rico 00931 ,","place":["United States"]},{"name":"Molecular Sciences Research Center, University of Puerto Rico , San Juan, Puerto Rico 00926 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6550-3851","authenticated-orcid":false,"given":"Bruno","family":"Marie","sequence":"additional","affiliation":[{"name":"Institute of Neurobiology, Medical Sciences Campus, University of Puerto Rico , San Juan, Puerto Rico 00901 ,","place":["United States"]},{"name":"Molecular Sciences Research Center, University of Puerto Rico , San Juan, Puerto Rico 00926 ,","place":["United States"]},{"name":"Department of Anatomy and Neurobiology, Medical Sciences Campus, University of Puerto Rico , San Juan, Puerto Rico 00921 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"2026100708030990900_iyag226-B1","doi-asserted-by":"publisher","first-page":"2881","DOI":"10.1016\/j.cub.2025.05.024","article-title":"Balanced synapse-to-synapse short-term plasticity ensures constant transmitter release.","volume":"35","author":"Aghi","year":"2025","journal-title":"Curr Biol."},{"key":"2026100708030990900_iyag226-B2","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1042\/BJ20060166","article-title":"Control and regulation of the cellular responses to cold shock: the responses in yeast and mammalian systems.","volume":"397","author":"Al-Fageeh","year":"2006","journal-title":"Biochem J."},{"key":"2026100708030990900_iyag226-B3","doi-asserted-by":"publisher","first-page":"2203","DOI":"10.1523\/JNEUROSCI.1375-16.2017","article-title":"Cortactin is a regulator of activity-dependent synaptic plasticity controlled by wingless.","volume":"37","author":"Alicea","year":"2017","journal-title":"J Neurosci."},{"key":"2026100708030990900_iyag226-B4","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1002\/hipo.450050602","article-title":"Brain temperature and hippocampal function.","volume":"5","author":"Andersen","year":"1995","journal-title":"Hippocampus"},{"key":"2026100708030990900_iyag226-B5","doi-asserted-by":"publisher","first-page":"6270","DOI":"10.1523\/jneurosci.0925-20.2020","article-title":"Synaptic plasticity induced by differential manipulation of tonic and phasic motoneurons in drosophila.","volume":"40","author":"Aponte-Santiago","year":"2020","journal-title":"J Neurosci."},{"key":"2026100708030990900_iyag226-B6","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1016\/j.conb.2018.07.002","article-title":"An axonal stress response pathway: degenerative and regenerative signaling by DLK","volume":"53","author":"Asghari Adib","year":"2018","journal-title":"Curr Opin Neurobiol."},{"key":"2026100708030990900_iyag226-B7","doi-asserted-by":"publisher","first-page":"1008","DOI":"10.1002\/neu.480240803","article-title":"Differential ultrastructure of synaptic terminals on ventral longitudinal abdominal muscles in Drosophila larvae.","volume":"24","author":"Atwood","year":"1993","journal-title":"J Neurobiol."},{"key":"2026100708030990900_iyag226-B8","doi-asserted-by":"publisher","first-page":"572","DOI":"10.4161\/auto.7.6.14943","article-title":"P62, Ref(2)P and ubiquitinated proteins are conserved markers of neuronal aging, aggregate formation and progressive autophagic defects.","volume":"7","author":"Bartlett","year":"2011","journal-title":"Autophagy"},{"key":"2026100708030990900_iyag226-B9","doi-asserted-by":"publisher","first-page":"17937","DOI":"10.1523\/JNEUROSCI.6075-11.2013","article-title":"Retrograde BMP signaling at the synapse: a permissive signal for synapse maturation and activity-dependent plasticity.","volume":"33","author":"Berke","year":"2013","journal-title":"J Neurosci."},{"key":"2026100708030990900_iyag226-B10","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1016\/j.cell.2007.10.048","article-title":"Growth arrest and autophagy are required for salivary gland cell degradation in Drosophila.","volume":"131","author":"Berry","year":"2007","journal-title":"Cell"},{"key":"2026100708030990900_iyag226-B11","doi-asserted-by":"publisher","first-page":"1318","DOI":"10.1038\/s41467-019-09262-2","article-title":"Autophagy within the mushroom body protects from synapse aging in a non-cell autonomous manner.","volume":"10","author":"Bhukel","year":"2019","journal-title":"Nat Commun."},{"key":"2026100708030990900_iyag226-B12","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.ydbio.2005.01.002","article-title":"JNK signaling pathway required for wound healing in regenerating Drosophila wing imaginal discs.","volume":"280","author":"Bosch","year":"2005","journal-title":"Dev Biol."},{"key":"2026100708030990900_iyag226-B13","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1016\/0040-8166(88)90047-X","article-title":"Maturation and degeneration of the fat body in the Drosophila larva and pupa as revealed by morphometric analysis.","volume":"20","author":"Butterworth","year":"1988","journal-title":"Tissue Cell."},{"key":"2026100708030990900_iyag226-B14","doi-asserted-by":"publisher","first-page":"101181","DOI":"10.1016\/j.redox.2019.101181","article-title":"Enhanced autophagic-lysosomal activity and increased BAG3-mediated selective macroautophagy as adaptive response of neuronal cells to chronic oxidative stress.","volume":"24","author":"Chakraborty","year":"2019","journal-title":"Redox Biol."},{"key":"2026100708030990900_iyag226-B15","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1002\/neu.480250204","article-title":"Developmental temperature selectively regulates a voltage-activated potassium current in Drosophila.","volume":"25","author":"Chopra","year":"1994","journal-title":"J Neurobiol."},{"key":"2026100708030990900_iyag226-B16","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.neuron.2006.05.026","article-title":"Highwire restrains synaptic growth by attenuating a MAP kinase signal.","volume":"51","author":"Collins","year":"2006","journal-title":"Neuron"},{"key":"2026100708030990900_iyag226-B17","doi-asserted-by":"publisher","first-page":"2849","DOI":"10.1038\/s41467-021-23133-9","article-title":"NMDAR-dependent long-term depression is associated with increased short term plasticity through autophagy mediated loss of PSD-95.","volume":"12","author":"Compans","year":"2021","journal-title":"Nat Commun."},{"key":"2026100708030990900_iyag226-B18","doi-asserted-by":"publisher","first-page":"RP108282","DOI":"10.7554\/eLife.108282","article-title":"A stochastic RNA editing process targets a select number of sites in individual Drosophila glutamatergic motoneurons","volume":"14","author":"Crane","year":"2025","journal-title":"eLife"},{"key":"2026100708030990900_iyag226-B19","doi-asserted-by":"publisher","first-page":"1350","DOI":"10.1038\/ejhg.2008.111","article-title":"Identification of human haploinsufficient genes and their genomic proximity to segmental duplications.","volume":"16","author":"Dang","year":"2008","journal-title":"Eur J Hum Genet."},{"key":"2026100708030990900_iyag226-B20","doi-asserted-by":"publisher","first-page":"1915","DOI":"10.1534\/genetics.104.036871","article-title":"Mechanisms of haploinsufficiency revealed by genome-wide profiling in yeast","volume":"169","author":"Deutschbauer","year":"2005","journal-title":"Genetics"},{"key":"2026100708030990900_iyag226-B21","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1038\/35086595","article-title":"Ubiquitination-dependent mechanisms regulate synaptic growth and function.","volume":"412","author":"DiAntonio","year":"2001","journal-title":"Nature"},{"key":"2026100708030990900_iyag226-B22","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1016\/j.neuron.2015.12.023","article-title":"Axon self-destruction: new links among SARM1, MAPKs, and NAD+ metabolism.","volume":"89","author":"Gerdts","year":"2016","journal-title":"Neuron"},{"key":"2026100708030990900_iyag226-B23","doi-asserted-by":"publisher","first-page":"4051","DOI":"10.1523\/JNEUROSCI.2601-18.2019","article-title":"A screen for synaptic growth mutants reveals mechanisms that stabilize synaptic strength.","volume":"39","author":"Goel","year":"2019","journal-title":"J Neurosci."},{"key":"2026100708030990900_iyag226-B24","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1016\/S0960-9822(09)00451-5","article-title":"The Drosophila tumor suppressor gene, dlg, is involved in structural plasticity at a glutamatergic synapse.","volume":"6","author":"Guan","year":"1996","journal-title":"Curr Biol."},{"key":"2026100708030990900_iyag226-B25","doi-asserted-by":"publisher","first-page":"802","DOI":"10.1126\/science.1165527","article-title":"Axon regeneration requires a conserved MAP kinase pathway.","volume":"323","author":"Hammarlund","year":"2009","journal-title":"Science"},{"key":"2026100708030990900_iyag226-B26","doi-asserted-by":"publisher","first-page":"3527","DOI":"10.1128\/MCB.18.6.3527","article-title":"A conserved p38 mitogen-activated protein kinase pathway regulates Drosophila immunity gene expression.","volume":"18","author":"Han","year":"1998","journal-title":"Mol Cell Biol."},{"key":"2026100708030990900_iyag226-B27","doi-asserted-by":"publisher","first-page":"4598","DOI":"10.1523\/JNEUROSCI.0046-23.2023","article-title":"Physiologic and nanoscale distinctions define glutamatergic synapses in tonic vs phasic neurons.","volume":"43","author":"He","year":"2023","journal-title":"J Neurosci"},{"key":"2026100708030990900_iyag226-B28","doi-asserted-by":"publisher","first-page":"12460","DOI":"10.1523\/JNEUROSCI.0222-12.2012","article-title":"Characterization of Drosophila larval crawling at the level of organism, segment, and somatic body wall musculature.","volume":"32","author":"Heckscher","year":"2012","journal-title":"J Neurosci"},{"key":"2026100708030990900_iyag226-B29","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1006\/dbio.2000.9983","article-title":"Single-cell analysis of Drosophila larval neuromuscular synapses.","volume":"229","author":"Hoang","year":"2001","journal-title":"Dev Biol."},{"key":"2026100708030990900_iyag226-B30","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1113\/jphysiol.1952.sp004716","article-title":"Measurement of current\u2013voltage relations in the membrane of the giant axon of Loligo.","volume":"116","author":"Hodgkin","year":"1952","journal-title":"J Physiol."},{"key":"2026100708030990900_iyag226-B31","doi-asserted-by":"publisher","first-page":"24994","DOI":"10.1074\/jbc.272.40.24994","article-title":"MKK7 is a stress-activated mitogen-activated protein kinase kinase functionally related to hemipterous.","volume":"272","author":"Holland","year":"1997","journal-title":"J Biol Chem."},{"key":"2026100708030990900_iyag226-B32","doi-asserted-by":"publisher","first-page":"2817","DOI":"10.1523\/JNEUROSCI.2002-19.2020","article-title":"Structural remodeling of active zones is associated with synaptic homeostasis.","volume":"40","author":"Hong","year":"2020","journal-title":"J Neurosci."},{"key":"2026100708030990900_iyag226-B33","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1016\/j.neuron.2020.11.012","article-title":"Injury-induced inhibition of bystander neurons requires dSarm and signaling from Glia.","volume":"109","author":"Hsu","year":"2021","journal-title":"Neuron"},{"key":"2026100708030990900_iyag226-B34","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1017\/9781009157940.005","volume-title":"Global warming of 1.5 \u00b0C: IPCC special report on impacts of global warming of 1.5 \u00b0C above pre-industrial levels in context of strengthening response to climate change, sustainable development, and efforts to eradicate poverty","author":"Intergovernmental Panel on Climate Change (IPCC)","year":"2022"},{"key":"2026100708030990900_iyag226-B35","volume-title":"IPCC, 2023: climate change 2023: synthesis report. A report of the intergovernmental panel on climate change. Contribution of working groups I, II and III to the sixth assessment report of the intergovernmental panel on climate change.","author":"IPCC","year":"2023"},{"key":"2026100708030990900_iyag226-B36","doi-asserted-by":"publisher","first-page":"3554","DOI":"10.1016\/j.neuron.2023.07.019","article-title":"Molecular logic of synaptic diversity between Drosophila tonic and phasic motoneurons.","volume":"111","author":"Jetti","year":"2023","journal-title":"Neuron"},{"key":"2026100708030990900_iyag226-B37","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1002\/neu.480240804","article-title":"Ultrastructure of neuromuscular junctions in Drosophila: comparison of wild type and mutants with increased excitability.","volume":"24","author":"Jia","year":"1993","journal-title":"J Neurobiol."},{"key":"2026100708030990900_iyag226-B38","doi-asserted-by":"publisher","first-page":"710","DOI":"10.1523\/JNEUROSCI.09-02-00710.1989","article-title":"Stereotypic morphology of glutamatergic synapses on identified muscle cells of Drosophila larvae.","volume":"9","author":"Johansen","year":"1989","journal-title":"J Neurosci."},{"key":"2026100708030990900_iyag226-B39","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1146\/annurev.neuro.19.1.545","article-title":"The Drosophila neuromuscular junction: a model system for studying synaptic development and function.","volume":"19","author":"Keshishian","year":"1996","journal-title":"Annu Rev Neurosci."},{"key":"2026100708030990900_iyag226-B40","doi-asserted-by":"publisher","first-page":"RP96592","DOI":"10.7554\/eLife.96592","article-title":"Rab11 suppresses neuronal stress signaling by localizing dual leucine zipper kinase to axon terminals for protein turnover.","volume":"13","author":"Kim","year":"2024","journal-title":"eLife"},{"key":"2026100708030990900_iyag226-B41","doi-asserted-by":"publisher","first-page":"056029","DOI":"10.1088\/1741-2552\/ac9339","article-title":"Thermal effects on neurons during stimulation of the brain.","volume":"19","author":"Kim","year":"2022","journal-title":"J Neural Eng."},{"key":"2026100708030990900_iyag226-B42","doi-asserted-by":"publisher","first-page":"1325","DOI":"10.1038\/s41467-020-14781-4","article-title":"Autophagy-dependent filopodial kinetics restrict synaptic partner choice during Drosophila brain wiring","volume":"11","author":"Kiral","year":"2020","journal-title":"Nat Commun"},{"key":"2026100708030990900_iyag226-B43","doi-asserted-by":"publisher","first-page":"110145","DOI":"10.1016\/j.celrep.2021.110145","article-title":"Brain connectivity inversely scales with developmental temperature in Drosophila.","volume":"37","author":"Kiral","year":"2021","journal-title":"Cell Rep."},{"key":"2026100708030990900_iyag226-B44","doi-asserted-by":"publisher","first-page":"12764","DOI":"10.1523\/JNEUROSCI.5160-12.2013","article-title":"Independent pathways downstream of the Wnd\/DLK MAPKKK regulate synaptic structure, axonal transport, and injury signaling.","volume":"33","author":"Klinedinst","year":"2013","journal-title":"J Neurosci"},{"key":"2026100708030990900_iyag226-B45","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1146\/annurev-biophys-112221-074832","article-title":"The effects of temperature on cellular physiology.","volume":"51","author":"Knapp","year":"2022","journal-title":"Annu Rev Biophys."},{"key":"2026100708030990900_iyag226-B46","doi-asserted-by":"publisher","first-page":"1366","DOI":"10.1523\/JNEUROSCI.3889-05.2006","article-title":"Physiological temperatures reduce the rate of vesicle pool depletion and short-term depression via an acceleration of vesicle recruitment.","volume":"26","author":"Kushmerick","year":"2006","journal-title":"J Neurosci."},{"key":"2026100708030990900_iyag226-B47","doi-asserted-by":"publisher","first-page":"e0153627","DOI":"10.1371\/journal.pone.0153627","article-title":"Hypoxia induces autophagy through translational up-regulation of lysosomal proteins in human colon cancer cells.","volume":"11","author":"Lai","year":"2016","journal-title":"PLoS One"},{"key":"2026100708030990900_iyag226-B48","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1006\/dbio.2002.0784","article-title":"Steroid regulation of midgut cell death during Drosophila development.","volume":"250","author":"Lee","year":"2002","journal-title":"Dev Biol."},{"key":"2026100708030990900_iyag226-B49","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1016\/j.neuron.2007.09.009","article-title":"The ubiquitin ligase Phr1 regulates axon outgrowth through modulation of microtubule dynamics.","volume":"56","author":"Lewcock","year":"2007","journal-title":"Neuron"},{"key":"2026100708030990900_iyag226-B50","doi-asserted-by":"publisher","first-page":"e24271","DOI":"10.7554\/eLife.24271","article-title":"Restraint of presynaptic protein levels by Wnd\/DLK signaling mediates synaptic defects associated with the kinesin-3 motor Unc-104","volume":"6","author":"Li","year":"2017","journal-title":"eLife"},{"key":"2026100708030990900_iyag226-B51","doi-asserted-by":"publisher","first-page":"10083","DOI":"10.1038\/ncomms10083","article-title":"Cold-sensing regulates Drosophila growth through insulin-producing cells.","volume":"6","author":"Li","year":"2015","journal-title":"Nat Commun."},{"key":"2026100708030990900_iyag226-B52","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1002\/(SICI)1097-4695(200005)43:2&lt;186::AID-NEU8&gt;3.0.CO;2-N","article-title":"Identified motor terminals in Drosophila larvae show distinct differences in morphology and physiology","volume":"43","author":"Lnenicka","year":"2000","journal-title":"J Neurobiol"},{"key":"2026100708030990900_iyag226-B53","doi-asserted-by":"publisher","first-page":"2562","DOI":"10.1016\/j.cub.2016.07.032","article-title":"High-probability neurotransmitter release sites represent an energy-efficient design","volume":"26","author":"Lu","year":"2016","journal-title":"Curr Biol."},{"key":"2026100708030990900_iyag226-B54","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/j.modgep.2005.07.006","article-title":"The expression pattern of the Drosophila vesicular glutamate transporter: a marker protein for motoneurons and glutamatergic centers in the brain","volume":"6","author":"Mahr","year":"2006","journal-title":"Gene Expr Patterns"},{"key":"2026100708030990900_iyag226-B55","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.mcn.2012.10.009","article-title":"Adar is essential for optimal presynaptic function.","volume":"52","author":"Maldonado","year":"2013","journal-title":"Mol Cell Neurosci."},{"key":"2026100708030990900_iyag226-B56","doi-asserted-by":"publisher","first-page":"e0260553","DOI":"10.1371\/journal.pone.0260553","article-title":"A comparison of three different methods of eliciting rapid activity-dependent synaptic plasticity at the Drosophila NMJ.","volume":"16","author":"Maldonado-D\u00edaz","year":"2021","journal-title":"PLoS One"},{"key":"2026100708030990900_iyag226-B57","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/j.neuron.2004.07.001","article-title":"Dap160\/intersectin scaffolds the periactive zone to achieve high-fidelity endocytosis and normal synaptic growth.","volume":"43","author":"Marie","year":"2004","journal-title":"Neuron"},{"key":"2026100708030990900_iyag226-B58","doi-asserted-by":"publisher","first-page":"8071","DOI":"10.1523\/JNEUROSCI.5467-09.2010","article-title":"Synaptic homeostasis is consolidated by the cell fate gene gooseberry, a drosophila pax3\/7 homolog","volume":"30","author":"Marie","year":"2010","journal-title":"J Neurosci"},{"key":"2026100708030990900_iyag226-B59","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/S0896-6273(03)00426-4","article-title":"The BMP homolog Gbb provides a retrograde signal that regulates synaptic growth at the Drosophila neuromuscular junction.","volume":"39","author":"McCabe","year":"2003","journal-title":"Neuron"},{"key":"2026100708030990900_iyag226-B60","doi-asserted-by":"publisher","first-page":"e0155957","DOI":"10.1371\/journal.pone.0155957","article-title":"Genetic and environmental control of neurodevelopmental robustness in Drosophila.","volume":"11","author":"Mellert","year":"2016","journal-title":"PLoS One"},{"key":"2026100708030990900_iyag226-B61","doi-asserted-by":"publisher","first-page":"7481","DOI":"10.1523\/JNEUROSCI.1801-05.2005","article-title":"Strong effects of subphysiological temperature on the function and plasticity of mammalian presynaptic terminals.","volume":"25","author":"Micheva","year":"2005","journal-title":"J Neurosci"},{"key":"2026100708030990900_iyag226-B62","doi-asserted-by":"publisher","first-page":"10875","DOI":"10.1523\/JNEUROSCI.0164-08.2008","article-title":"Presynaptic local signaling by a canonical wingless pathway regulates development of the Drosophila neuromuscular junction.","volume":"28","author":"Miech","year":"2008","journal-title":"J Neurosci."},{"key":"2026100708030990900_iyag226-B63","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1038\/nn.2290","article-title":"A dual leucine kinase-dependent axon self-destruction program promotes Wallerian degeneration.","volume":"12","author":"Miller","year":"2009","journal-title":"Nat Neurosci."},{"key":"2026100708030990900_iyag226-B64","doi-asserted-by":"publisher","first-page":"470","DOI":"10.3390\/insects11080470","article-title":"Effects of temperature on lifespan of Drosophila melanogaster from different genetic backgrounds: links between metabolic rate and longevity.","volume":"11","author":"Mo\u0142o\u0144","year":"2020","journal-title":"Insects"},{"key":"2026100708030990900_iyag226-B65","doi-asserted-by":"publisher","first-page":"453","DOI":"10.4161\/auto.27442","article-title":"Atg17\/FIP200 localizes to perilysosomal Ref(2)P aggregates and promotes autophagy by activation of Atg1 in Drosophila.","volume":"10","author":"Nagy","year":"2014","journal-title":"Autophagy"},{"key":"2026100708030990900_iyag226-B66","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1016\/j.cell.2004.12.017","article-title":"Regulation of a DLK-1 and p38 MAP kinase pathway by the ubiquitin ligase RPM-1 is required for presynaptic development.","volume":"120","author":"Nakata","year":"2005","journal-title":"Cell"},{"key":"2026100708030990900_iyag226-B67","doi-asserted-by":"publisher","first-page":"1388","DOI":"10.1016\/j.neuron.2017.02.028","article-title":"Input-specific plasticity and homeostasis at the Drosophila larval neuromuscular junction.","volume":"93","author":"Newman","year":"2017","journal-title":"Neuron"},{"key":"2026100708030990900_iyag226-B68","doi-asserted-by":"publisher","first-page":"1065","DOI":"10.1083\/jcb.200711108","article-title":"Ref(2)P, the Drosophila melanogaster homologue of mammalian p62, is required for the formation of protein aggregates in adult brain.","volume":"180","author":"Nezis","year":"2008","journal-title":"J Cell Biol."},{"key":"2026100708030990900_iyag226-B69","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/S12868-016-0290-7\/FIGURES\/5","article-title":"The influence of postsynaptic structure on missing quanta at the Drosophila neuromuscular junction.","volume":"17","author":"Nguyen","year":"2016","journal-title":"BMC Neurosci."},{"key":"2026100708030990900_iyag226-B70","doi-asserted-by":"publisher","first-page":"2935","DOI":"10.1016\/j.cub.2016.08.061","article-title":"Temperature-robust neural function from activity-dependent ion channel regulation.","volume":"26","author":"O\u2019Leary","year":"2016","journal-title":"Curr Biol."},{"key":"2026100708030990900_iyag226-B71","doi-asserted-by":"publisher","first-page":"1061","DOI":"10.1038\/s41593-019-0422-3","article-title":"Thermal constraints on in vivo optogenetic manipulations.","volume":"22","author":"Owen","year":"2019","journal-title":"Nat Neurosci."},{"key":"2026100708030990900_iyag226-B72","doi-asserted-by":"publisher","first-page":"24131","DOI":"10.1074\/jbc.M702824200","article-title":"P62\/SQSTM1 binds directly to Atg8\/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy.","volume":"282","author":"Pankiv","year":"2007","journal-title":"J Biol Chem."},{"key":"2026100708030990900_iyag226-B73","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1146\/annurev.ge.27.120193.002253","article-title":"The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins.","volume":"27","author":"Parsell","year":"1993","journal-title":"Annu Rev Genet."},{"key":"2026100708030990900_iyag226-B74","doi-asserted-by":"publisher","first-page":"12611","DOI":"10.1523\/JNEUROSCI.2179-07.2007","article-title":"Temperature-dependent developmental plasticity of Drosophila neurons: cell-autonomous roles of membrane excitability, Ca2+ influx, and cAMP signaling.","volume":"27","author":"Peng","year":"2007","journal-title":"J Neurosci."},{"key":"2026100708030990900_iyag226-B75","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/S1369-5274(99)80031-9","article-title":"Cold-shock response and cold-shock proteins.","volume":"2","author":"Phadtare","year":"1999","journal-title":"Curr Opin Microbiol."},{"key":"2026100708030990900_iyag226-B76","doi-asserted-by":"publisher","first-page":"1344","DOI":"10.1126\/science.1193497","article-title":"Autophagy and metabolism.","volume":"330","author":"Rabinowitz","year":"2010","journal-title":"Science"},{"key":"2026100708030990900_iyag226-B77","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.devcel.2004.07.005","article-title":"Programmed autophagy in the Drosophila fat body is induced by ecdysone through regulation of the PI3K pathway.","volume":"7","author":"Rusten","year":"2004","journal-title":"Dev Cell."},{"key":"2026100708030990900_iyag226-B78","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1016\/s0896-6273(00)81168-x","article-title":"Rpm-1, a conserved neuronal gene that regulates targeting and synaptogenesis in C. elegans.","volume":"26","author":"Schaefer","year":"2000","journal-title":"Neuron"},{"key":"2026100708030990900_iyag226-B79","doi-asserted-by":"publisher","first-page":"1257","DOI":"10.1152\/jn.00298.2010","article-title":"Role of intrinsic properties in Drosophila motoneuron recruitment during fictive crawling.","volume":"104","author":"Schaefer","year":"2010","journal-title":"J Neurophysiol."},{"key":"2026100708030990900_iyag226-B80","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1016\/S0896-6273(00)80197-X","article-title":"Genetic dissection of structural and functional components of synaptic plasticity. I. Fasciclin II controls synaptic stabilization and growth.","volume":"17","author":"Schuster","year":"1996","journal-title":"Neuron"},{"key":"2026100708030990900_iyag226-B81","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.cub.2006.10.053","article-title":"Direct induction of autophagy by Atg1 inhibits cell growth and induces apoptotic cell death.","volume":"17","author":"Scott","year":"2007","journal-title":"Curr Biol."},{"key":"2026100708030990900_iyag226-B82","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1083\/jcb.200907109","article-title":"Autophagy promotes synapse development in Drosophila.","volume":"187","author":"Shen","year":"2009","journal-title":"J Cell Biol."},{"key":"2026100708030990900_iyag226-B83","doi-asserted-by":"publisher","first-page":"6546","DOI":"10.1523\/JNEUROSCI.23-16-06546.2003","article-title":"Experience-dependent strengthening of Drosophila neuromuscular junctions.","volume":"23","author":"Sigrist","year":"2003","journal-title":"J Neurosci."},{"key":"2026100708030990900_iyag226-B84","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1097\/WCO.0000000000000621","article-title":"Therapeutic opportunities and pitfalls in the treatment of axon degeneration.","volume":"31","author":"Simon","year":"2018","journal-title":"Curr Opin Neurol."},{"key":"2026100708030990900_iyag226-B85","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/j.devcel.2016.06.012","article-title":"KIF1A\/UNC-104 transports ATG-9 to regulate neurodevelopment and autophagy at synapses.","volume":"38","author":"Stavoe","year":"2016","journal-title":"Dev Cell."},{"key":"2026100708030990900_iyag226-B86","doi-asserted-by":"publisher","first-page":"115483","DOI":"10.1016\/j.celrep.2025.115483","article-title":"PDZD8 promotes autophagy at ER-lysosome membrane contact sites to regulate activity-dependent synaptic growth.","volume":"44","author":"Thakur","year":"2025","journal-title":"Cell Rep."},{"key":"2026100708030990900_iyag226-B87","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1038\/ng1314","article-title":"A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac.","volume":"36","author":"Thibault","year":"2004","journal-title":"Nat Genet."},{"key":"2026100708030990900_iyag226-B88","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1016\/0092-8674(84)90345-3","article-title":"Hsp70: nuclear concentration during environmental stress and cytoplasmic storage during recovery.","volume":"36","author":"Velazquez","year":"1984","journal-title":"Cell"},{"key":"2026100708030990900_iyag226-B89","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/S0896-6273(00)81166-6","article-title":"Highwire regulates synaptic growth in Drosophila.","volume":"26","author":"Wan","year":"2000","journal-title":"Neuron"},{"key":"2026100708030990900_iyag226-B90","doi-asserted-by":"publisher","first-page":"307","DOI":"10.3389\/fnins.2014.00307","article-title":"Brain temperature and its fundamental properties: a review for clinical neuroscientists.","volume":"8","author":"Wang","year":"2014","journal-title":"Front Neurosci."},{"key":"2026100708030990900_iyag226-B91","doi-asserted-by":"publisher","first-page":"1401","DOI":"10.1523\/JNEUROSCI.1492-20.2020","article-title":"Structural and functional synaptic plasticity induced by convergent synapse loss in the Drosophila neuromuscular circuit.","volume":"41","author":"Wang","year":"2021","journal-title":"J Neurosci"},{"key":"2026100708030990900_iyag226-B92","doi-asserted-by":"publisher","first-page":"e29338","DOI":"10.7554\/eLife.29338","article-title":"Atg9 antagonizes TOR signaling to regulate intestinal cell growth and epithelial homeostasis in Drosophila","volume":"6","author":"Wen","year":"2017","journal-title":"eLife"},{"key":"2026100708030990900_iyag226-B93","doi-asserted-by":"publisher","first-page":"20231305","DOI":"10.1098\/rspb.2023.1305","article-title":"Exploring the connection between autophagy and heat-stress tolerance in Drosophila melanogaster.","volume":"290","author":"Willot","year":"2023","journal-title":"Proc R Soc Lond B Biol Sci."},{"key":"2026100708030990900_iyag226-B94","doi-asserted-by":"publisher","first-page":"764","DOI":"10.1016\/j.neuron.2014.09.030","article-title":"A TRPV channel in Drosophila motor neurons regulates presynaptic resting Ca2+ levels, synapse growth, and synaptic transmission.","volume":"84","author":"Wong","year":"2014","journal-title":"Neuron"},{"key":"2026100708030990900_iyag226-B95","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/1749-8104-2-16","article-title":"DFsn collaborates with Highwire to down-regulate the Wallenda\/DLK kinase and restrain synaptic terminal growth.","volume":"2","author":"Wu","year":"2007","journal-title":"Neural Dev."},{"key":"2026100708030990900_iyag226-B96","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1083\/jcb.201006039","article-title":"Protein turnover of the Wallenda\/DLK kinase regulates a retrograde response to axonal injury.","volume":"191","author":"Xiong","year":"2010","journal-title":"J Cell Biol."},{"key":"2026100708030990900_iyag226-B97","doi-asserted-by":"publisher","first-page":"e1001440","DOI":"10.1371\/journal.pbio.1001440","article-title":"The highwire ubiquitin ligase promotes axonal degeneration by tuning levels of nmnat protein.","volume":"10","author":"Xiong","year":"2012","journal-title":"PLOS Biol"},{"key":"2026100708030990900_iyag226-B98","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1523\/ENEURO.0220-17.2017","article-title":"The maintenance of synaptic homeostasis at the Drosophila neuromuscular junction is reversible and sensitive to high temperature","volume":"4","author":"Yeates","year":"2017","journal-title":"eNeuro"},{"key":"2026100708030990900_iyag226-B99","doi-asserted-by":"publisher","first-page":"eadp9587","DOI":"10.1126\/sciadv.adp9587","article-title":"Impact of developmental temperature on neural growth, connectivity, and function.","volume":"11","author":"Z\u00fcfle","year":"2025","journal-title":"Sci Adv"}],"container-title":["GENETICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/genetics\/advance-article-pdf\/doi\/10.1093\/genetics\/iyag226\/70782572\/iyag226.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/genetics\/article-pdf\/234\/2\/iyag226\/70782572\/iyag226.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/genetics\/article-pdf\/234\/2\/iyag226\/70782572\/iyag226.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T12:03:28Z","timestamp":1791374608000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/genetics\/article\/doi\/10.1093\/genetics\/iyag226\/8770130"}},"subtitle":[],"editor":[{"given":"K","family":"Kaun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,25]]},"references-count":99,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,8,25]]},"published-print":{"date-parts":[[2026,10,7]]}},"URL":"https:\/\/doi.org\/10.1093\/genetics\/iyag226","relation":{},"ISSN":["1943-2631"],"issn-type":[{"value":"1943-2631","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,10]]},"published":{"date-parts":[[2026,8,25]]},"article-number":"iyag226"}}