{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T19:34:13Z","timestamp":1778960053610,"version":"3.51.4"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T00:00:00Z","timestamp":1704844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2021B1515140057"],"award-info":[{"award-number":["2021B1515140057"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82273622"],"award-info":[{"award-number":["82273622"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"BYHEALTH Nutrition and Health Research Foundation","award":["TY202101001"],"award-info":[{"award-number":["TY202101001"]}]},{"name":"Discipline Construction Project of Guangdong Medical University","award":["4SG21016G"],"award-info":[{"award-number":["4SG21016G"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Weight regain subsequent to weight reduction resulting from dietary interventions represents a prevalent phenomenon recognized as \u201cYo-yo dieting.\u201d However, the impact of prolonged Yo-yo dieting on health, especially in relation to the aging process, remains poorly understood. This study aimed to investigate the influence of Yo-yo dieting on the aging process in male Drosophila melanogaster that have been exposed to a high-calorie (HC) diet. Fruit flies were fed with either a consistent HC diet or an alternating regimen of HC and low-calorie diets every 3 days (referred to as \u201cYo-yo dieting\u201d) for a total of 24 days. Biochemical assays were utilized to quantify levels of oxidative stress and activities of the mitochondrial respiratory chain complexes. The frozen section staining method was employed to assess the presence of lipid droplets, reactive oxygen species, cellular viability, and mitochondrial abundance in tissues. Additionally, we examined the expression of key regulators involved in mitochondrial dynamics and biogenic signaling pathways. Yo-yo dieting resulted in an extension of the fruit flies\u2019 lifespan, concomitant with reduced body weight, decreased body protein content, and lower triglyceride levels compared to continuous a HC diet feeding. Furthermore, Yo-yo dieting ameliorated impairments in motility and intestinal barrier function. Importantly, it improved mitochondrial function and upregulated the expression of essential mitochondrial fusion proteins, namely mitofusin 1 and mitofusin 2, optic atrophy 1, and peroxisome proliferator-activated receptor-\u03b3 coactivator-1\u03b1. Therefore, the practice of Yo-yo dieting extends the lifespan of fruit flies by modulating mitochondrial dynamics and the associated biogenic signaling pathways.<\/jats:p>","DOI":"10.1093\/gerona\/glae014","type":"journal-article","created":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T05:53:28Z","timestamp":1704779608000},"source":"Crossref","is-referenced-by-count":1,"title":["Yo-Yo Dieting Delays Male <i>Drosophila melanogaster<\/i> Aging Through Enhanced Mitochondrial Function, Relative to Sustained High-Calorie Diet Feeding"],"prefix":"10.1093","volume":"79","author":[{"given":"Mengliu","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Nutrition, School of Public Health, Guangdong Medical University , Zhanjiang ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianhua","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health, Guangdong Medical University , Zhanjiang ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutong","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health, Guangdong Medical University , Zhanjiang ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiqing","family":"Mai","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health, Guangdong Medical University , Zhanjiang ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanhan","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health, Guangdong Medical University , Zhanjiang ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health, Guangdong Medical University , Zhanjiang ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haimei","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health, Guangdong Medical University , Zhanjiang ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enqin","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health, Guangdong Medical University , Zhanjiang ,","place":["China"]},{"name":"Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University , Dongguan ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7837-0392","authenticated-orcid":false,"given":"Honghui","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health, Guangdong Medical University , Zhanjiang ,","place":["China"]},{"name":"Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University , Dongguan ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,1,10]]},"reference":[{"key":"2025031813481142900_CIT0001","first-page":"75","article-title":"Supplementing glycine and N-acetylcysteine (GlyNAC) in older adults improves glutathione deficiency, oxidative stress, mitochondrial dysfunction, inflammation, physical function, and aging hallmarks: a randomized clinical trial","volume-title":"J Gerontol A Biol Sci Med Sci.","author":"Kumar","year":"2023"},{"key":"2025031813481142900_CIT0002","first-page":"e13493","article-title":"How high-fat diet affects bone in mice: a systematic review and meta-analysis","volume-title":"Obes Rev.","author":"Zhang","year":"2022"},{"key":"2025031813481142900_CIT0003","first-page":"1529","article-title":"The long-term effect of energy restricted diets for treating obesity","volume-title":"Obesity (Silver Spring)","author":"Langeveld","year":"2015"},{"key":"2025031813481142900_CIT0004","first-page":"1673","article-title":"Yo-yo dieting is better than none","volume-title":"Obesity (Silver Spring)","author":"Di Germanio","year":"2018"},{"key":"2025031813481142900_CIT0005","first-page":"7","article-title":"Dieting and weight cycling as risk factors for cardiometabolic diseases: who is really at risk","volume-title":"Obes Rev.","author":"Montani","year":"2015"},{"key":"2025031813481142900_CIT0006","first-page":"333","article-title":"Prevalence of weight cycling and its relation to health indicators in Finland","volume-title":"Obes Res.","author":"Lahti-Koski","year":"2005"},{"key":"2025031813481142900_CIT0007","first-page":"230","article-title":"Increased risk of diabetes development in individuals with weight cycling over 4 years: the Kangbuk Samsung health study","volume-title":"Diabetes Res Clin Pract.","author":"Rhee","year":"2018"},{"key":"2025031813481142900_CIT0008","first-page":"728","article-title":"Body-weight fluctuation was associated with increased risk for cardiovascular disease, all-cause and cardiovascular mortality: a systematic review and meta-analysis","volume-title":"Front Endocrinol (Lausanne)","author":"Zou","year":"2019"},{"key":"2025031813481142900_CIT0009","first-page":"1332","article-title":"Body-weight fluctuations and outcomes in coronary disease","volume-title":"N Engl J Med.","author":"Bangalore","year":"2017"},{"key":"2025031813481142900_CIT0010","first-page":"1733","article-title":"Weight cycling increases longevity compared with sustained obesity in mice","volume-title":"Obesity (Silver Spring)","author":"Smith","year":"2018"},{"key":"2025031813481142900_CIT0011","first-page":"R1","article-title":"Mitochondrial dysfunction and insulin resistance: an update","volume-title":"Endocr Connect","author":"Montgomery","year":"2015"},{"key":"2025031813481142900_CIT0012","first-page":"26","article-title":"Mitochondrial dysfunction in obesity","volume-title":"Life Sci.","author":"de Mello A","year":"2018"},{"key":"2025031813481142900_CIT0013","first-page":"69","article-title":"Regulation of mitochondrial biogenesis","volume-title":"Essays Biochem.","author":"Jornayvaz","year":"2010"},{"key":"2025031813481142900_CIT0014","first-page":"491","article-title":"Mitochondrial dynamics in the regulation of nutrient utilization and energy expenditure","volume-title":"Cell Metab.","author":"Liesa","year":"2013"},{"key":"2025031813481142900_CIT0015","first-page":"3135","article-title":"Impaired mitochondrial biogenesis in adipose tissue in acquired obesity","volume-title":"Diabetes.","author":"Heinonen","year":"2015"},{"key":"2025031813481142900_CIT0016","first-page":"789","article-title":"Mitochondrial dysfunction results from oxidative stress in the skeletal muscle of diet-induced insulin-resistant mice","volume-title":"J Clin Invest.","author":"Bonnard","year":"2008"},{"key":"2025031813481142900_CIT0017","first-page":"210","article-title":"Green tea polyphenols require the mitochondrial iron transporter, mitoferrin, for lifespan extension in Drosophila melanogaster","volume-title":"Arch Insect Biochem Physiol.","author":"Lopez","year":"2016"},{"key":"2025031813481142900_CIT0018","first-page":"738","article-title":"Angelica keiskei impacts the lifespan and healthspan of Drosophila melanogaster in a sex and strain-dependent manner","volume-title":"Pharmaceuticals (Basel)","author":"Jafari","year":"2023"},{"key":"2025031813481142900_CIT0019","first-page":"307","article-title":"Drosophila melanogaster as a model organism for obesity and type-2 diabetes mellitus by applying high-sugar and high-fat diets","volume-title":"Biomolecules","author":"Baenas","year":"2022"},{"key":"2025031813481142900_CIT0020","first-page":"1529","article-title":"Synphilin-1 alters metabolic homeostasis in a novel Drosophila obesity model","volume-title":"Int J Obes (Lond).","author":"Liu","year":"2012"},{"key":"2025031813481142900_CIT0021","first-page":"e0230970","article-title":"Predicting death by the loss of intestinal function","volume-title":"PLoS One.","author":"Bitner","year":"2020"},{"key":"2025031813481142900_CIT0022","first-page":"2671","article-title":"Mitochondrial depolarization after acute ethanol treatment drives mitophagy in living mice","volume-title":"Autophagy","author":"Samuvel","year":"2022"},{"key":"2025031813481142900_CIT0023","first-page":"e13319","article-title":"Taurine represses age-associated gut hyperplasia in Drosophila via counteracting endoplasmic reticulum stress","volume-title":"Aging Cell.","author":"Du","year":"2021"},{"key":"2025031813481142900_CIT0024","first-page":"240","article-title":"Preventing age-related decline of gut compartmentalization limits microbiota dysbiosis and extends lifespan","volume-title":"Cell Host Microbe.","author":"Li","year":"2016"},{"key":"2025031813481142900_CIT0025","first-page":"535","article-title":"Quantifying Drosophila food intake: comparative analysis of current methodology","volume-title":"Nat Methods.","author":"Deshpande","year":"2014"},{"key":"2025031813481142900_CIT0026","first-page":"F527","article-title":"High-calorie diet results in reversible obesity-related glomerulopathy in adult zebrafish regardless of dietary fat","volume-title":"Am J Physiol Renal Physiol.","author":"Zeitler","year":"2022"},{"key":"2025031813481142900_CIT0027","first-page":"1342","article-title":"Fasting-mimicking diet prevents high-fat diet effect on cardiometabolic risk and lifespan","volume-title":"Nat Metab.","author":"Mishra","year":"2021"},{"key":"2025031813481142900_CIT0028","first-page":"1495","article-title":"Calorie restriction with or without time-restricted eating in weight loss","volume-title":"N Engl J Med.","author":"Liu","year":"2022"},{"key":"2025031813481142900_CIT0029","first-page":"e76","article-title":"Calorie restriction increases muscle mitochondrial biogenesis in healthy humans","volume-title":"PLoS Med.","author":"Civitarese","year":"2007"},{"key":"2025031813481142900_CIT0030","first-page":"573","article-title":"Mitochondrial H2O2 emission and cellular redox state link excess fat intake to insulin resistance in both rodents and humans","volume-title":"J Clin Invest.","author":"Anderson","year":"2009"},{"key":"2025031813481142900_CIT0031","first-page":"381","article-title":"High-fat diet induces skeletal muscle oxidative stress in a fiber type-dependent manner in rats","volume-title":"Free Radic Biol Med.","author":"Pinho","year":"2017"},{"key":"2025031813481142900_CIT0032","first-page":"838","article-title":"The NAD(+) precursor nicotinamide riboside enhances oxidative metabolism and protects against high-fat diet-induced obesity","volume-title":"Cell Metab.","author":"Cant\u00f3","year":"2012"},{"key":"2025031813481142900_CIT0033","first-page":"1388","article-title":"A mouse model of pathological small intestinal epithelial cell apoptosis and shedding induced by systemic administration of lipopolysaccharide","volume-title":"Dis Model Mech","author":"Williams","year":"2013"},{"key":"2025031813481142900_CIT0034","first-page":"e13379","article-title":"Loss of Fis1 impairs proteostasis during skeletal muscle aging in Drosophila","volume-title":"Aging Cell.","author":"Lee","year":"2021"},{"key":"2025031813481142900_CIT0035","first-page":"e158447","article-title":"Mitochondrial dysfunction in cell senescence and aging","volume-title":"J Clin Invest.","author":"Miwa","year":"2022"},{"key":"2025031813481142900_CIT0036","first-page":"659","article-title":"Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in mitochondrial fission","volume-title":"Mol Biol Cell.","author":"Los\u00f3n","year":"2013"},{"key":"2025031813481142900_CIT0037","first-page":"933","article-title":"Structure, function, and regulation of mitofusin-2 in health and disease","volume-title":"Biol Rev Camb Philos Soc.","author":"Chandhok","year":"2018"},{"key":"2025031813481142900_CIT0038","first-page":"309","article-title":"Mitochondrial fission contributes to mitochondrial dysfunction and insulin resistance in skeletal muscle","volume-title":"Mol Cell Biol.","author":"Jheng","year":"2012"},{"key":"2025031813481142900_CIT0039","first-page":"857","article-title":"Determinants and outcomes of mitochondrial dynamics","volume-title":"Mol Cell.","author":"Quintana-Cabrera","year":"2023"},{"key":"2025031813481142900_CIT0040","first-page":"112","article-title":"Mitochondrial dynamics proteins as emerging drug targets","volume-title":"Trends Pharmacol Sci.","author":"Zacharioudakis","year":"2023"}],"container-title":["The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/biomedgerontology\/advance-article-pdf\/doi\/10.1093\/gerona\/glae014\/55381258\/glae014.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biomedgerontology\/article-pdf\/79\/4\/glae014\/56910015\/glae014.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biomedgerontology\/article-pdf\/79\/4\/glae014\/56910015\/glae014.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T13:48:23Z","timestamp":1742305703000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biomedgerontology\/article\/doi\/10.1093\/gerona\/glae014\/7515087"}},"subtitle":[],"editor":[{"given":"Gustavo","family":"Duque","sequence":"additional","affiliation":[{"name":"(Biological Sciences Section)"}],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,1,10]]},"references-count":40,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4,1]]}},"URL":"https:\/\/doi.org\/10.1093\/gerona\/glae014","relation":{},"ISSN":["1079-5006","1758-535X"],"issn-type":[{"value":"1079-5006","type":"print"},{"value":"1758-535X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,4,1]]},"published":{"date-parts":[[2024,1,10]]},"article-number":"glae014"}}