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The decline of type H vessels contributes to bone loss in the aging process. Aging is accompanied by the accumulation of advanced oxidation protein products (AOPPs). However, whether AOPP accumulation is involved in age-related decline of type H vessels is unclear. Here, we show that the increase of AOPP levels in plasma and bone was correlated with the decline of type H vessels and loss of bone mass in old mice. Exposure of microvascular endothelial cells to AOPPs significantly inhibited cell proliferation, migration, and tube formation; increased NADPH oxidase activity and excessive reactive oxygen species generation; upregulated the expression of vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1; and eventually impaired angiogenesis, which was alleviated by redox modulator N-acetylcysteine and NADPH oxidase inhibitor apocynin. Furthermore, reduced AOPP accumulation by NAC treatment was able to alleviate significantly the decline of type H vessels, bone mass loss, and deterioration of bone microstructure in old mice. Collectively, these findings suggest that AOPPs accumulation contributes to the decline of type H vessels in the aging process, and illuminate a novel potential mechanism underlying age-related bone loss.<\/jats:p>","DOI":"10.1093\/gerona\/glae271","type":"journal-article","created":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T09:10:03Z","timestamp":1730970603000},"source":"Crossref","is-referenced-by-count":1,"title":["Accumulation of Advanced Oxidation Protein Products Promotes Age-Related Decline of Type H Vessels in Bone"],"prefix":"10.1093","volume":"80","author":[{"given":"Kai","family":"Zhao","sequence":"first","affiliation":[{"name":"Division of Spine Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical University , Guangzhou ,","place":["People\u2019s Republic of China"]},{"name":"Department of Orthopaedics, First Affiliated Hospital of Gannan Medical University , Ganzhou ,","place":["People\u2019s Republic of 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