{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T15:14:01Z","timestamp":1764170041090,"version":"3.46.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"K&D Program of the Sichuan Science and Technology Department","award":["2020YFS0573"],"award-info":[{"award-number":["2020YFS0573"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sarcopenia, associated with systemic inflammation, respiratory diseases, and known gut dysbiosis, poses a significant health burden. However, the role of the nasopharyngeal and oropharyngeal (NP\/OP) microbiome, a critical respiratory-digestive interface, in its pathogenesis remains unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>From a cohort of 830 nursing home residents with a sarcopenia prevalence of 61%, we conducted a nested case-control study. Sixty individuals with sarcopenia were propensity-score matched 1:1 with 60 non-sarcopenic controls (N\u2009=\u2009120; 50% male). NP\/OP swabs underwent full-length 16S rRNA gene sequencing to assess microbial composition and function.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Individuals with sarcopenia exhibited significantly lower OP microbial \u03b1-diversity (Shannon p\u2009=\u2009.016), which remained robust after multivariable adjustment (Shannon p\u2009&amp;lt;\u2009.05). NP diversity was unchanged. Sarcopenia was associated with an NP\/OP microbial profile suggesting increased pro-inflammatory potential: enrichment of Moraxella (NP, linear discriminant analysis [LDA]\u2009&amp;gt;\u20092) and Haemophilus, Lactobacillus amylovorus, Listeriaceae (OP, LDA\u2009&amp;gt;\u20092), alongside depletion of potentially protective taxa (Pasteurellaceae, Alloprevotella tannerae, Prevotella aurantiaca, Eubacterium, Lachnoanaerobaculum) in controls. Specifically, increased Moraxella lincolnii (odds ratio [OR]\u2009=\u20091.068, p\u2009&amp;lt;\u2009.05) and decreased Eubacterium (OR\u2009=\u20090.968, p\u2009&amp;lt;\u2009.05) were associated with sarcopenia. Functionally, pathways related to lipopolysaccharide (LPS) biosynthesis and saturated fatty acid metabolism were upregulated in sarcopenia, while short-chain fatty acid (SCFA) and tryptophan metabolism were reduced.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Oropharyngeal microbial dysbiosis, characterized by lower diversity and a pro-inflammatory signature, is associated with sarcopenia. These findings highlight a potential relationship between the upper respiratory tract microbial environment and sarcopenia, a connection previously underappreciated. Understanding the interplay within the respiratory\u2013gut\u2013muscle axis may offer new perspectives on sarcopenia pathophysiology and its links with respiratory diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/gerona\/glaf201","type":"journal-article","created":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T11:44:21Z","timestamp":1757763861000},"source":"Crossref","is-referenced-by-count":0,"title":["Oropharyngeal microbiome alterations in sarcopenia: a nested case-control study in older adults"],"prefix":"10.1093","volume":"80","author":[{"given":"Runjie","family":"Li","sequence":"first","affiliation":[{"name":"Center of Gerontology and Geriatrics, West China Hospital, Sichuan University , Chengdu,","place":["China"]},{"name":"Department of Geriatrics, Ya\u2019an People\u2019s Hospital , 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