{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:01:10Z","timestamp":1753876870222,"version":"3.41.2"},"reference-count":100,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T00:00:00Z","timestamp":1720742400000},"content-version":"vor","delay-in-days":193,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001792","name":"James Cook University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001792","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005853","name":"Universidad de Chile","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005853","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Cobia (Rachycentron canadum) is the only member of the Rachycentridae family and exhibits considerable sexual dimorphism in growth rate. Sex determination in teleosts has been a long-standing basic biological question, and the molecular mechanisms of sex determination\/differentiation in cobia are completely unknown.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Here, we reported 2 high-quality, chromosome-level annotated male and female cobia genomes with assembly sizes of 586.51 Mb (contig\/scaffold N50: 86.0\u00a0kb\/24.3 Mb) and 583.88 Mb (79.9\u00a0kb\/22.5 Mb), respectively. Synteny inference among perciform genomes revealed that cobia and the remora Echeneis naucrates were sister groups. Further, whole-genome resequencing of 31 males and 60 females, genome-wide association study, and sequencing depth analysis identified 3 short male-specific regions within a 10.7-kb continuous genomic region on male chromosome 18, which hinted at an undifferentiated sex chromosome system with a putative XX\/XY mode of sex determination in cobia. Importantly, the only 2 genes within\/between the male-specific regions, epoxide hydrolase 1 (ephx1, renamed cephx1y) and transcription factor 24 (tcf24, renamed ctcf24y), showed testis-specific\/biased gene expression, whereas their counterparts cephx1x and ctf24x, located in female chromosome 18, were similarly expressed in both sexes. In addition, male-specific PCR targeting the cephx1y gene revealed that this genomic feature is conserved in cobia populations from Panama, Brazil, Australia, and Japan.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>The first comprehensive genomic survey presented here is a valuable resource for future studies on cobia population structure and dynamics, conservation, and evolutionary history. Furthermore, it establishes evidence of putative male heterogametic regions with 2 genes playing a potential role in the sex determination of the species, and it provides further support for the rapid evolution of sex-determining mechanisms in teleost fish.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/gigascience\/giae034","type":"journal-article","created":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T11:50:10Z","timestamp":1720785010000},"source":"Crossref","is-referenced-by-count":2,"title":["Exploring the cobia (<i>Rachycentron canadum<\/i>) genome: unveiling putative male heterogametic regions and identification of sex-specific markers"],"prefix":"10.1093","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4229-791X","authenticated-orcid":false,"given":"Xueyan","family":"Shen","sequence":"first","affiliation":[{"name":"Tropical Futures Institute, James Cook University Singapore , 387380 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