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While several current matrix-assisted laser desorption\/ionization mass spectrometry imaging (MSI) instruments are capable of localizing numerous metabolites at high spatial and spectral resolution, most MSI data are acquired at the MS1 level only. Assigning molecular identities based on MS1 data presents significant analytical and computational challenges, as the inherent limitations of MS1 data preclude confident annotations beyond the sum formula level.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>To enable future advancements of computational lipid annotation tools, well-characterized benchmark\u2014or ground-truth\u2014datasets are crucial, which exceed the scope of synthetic data or data derived from mimetic tissue models. To this end, we provide 2 sulfatide-centered, biology-driven magnetic resonance MSI (MR-MSI) datasets at different mass resolving powers that characterize lipids in a mouse model of human metachromatic dystrophy. These data include an ultra-high-resolution (R \u223c1,230,000) quantum cascade laser mid-infrared imaging-guided MR-MSI dataset that enables isotopic fine structure analysis and therefore enhances the level of confidence substantially. To highlight the usefulness of the data, we compared 118 manual sulfatide annotations with the number of decoy database-controlled sulfatide annotations performed in Metaspace (67 at a false discovery rate &amp;lt;10%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Overall, our datasets can be used to benchmark annotation algorithms, validate spatial biomarker discovery pipelines, and serve as a reference for future studies that explore sulfatide metabolism and its spatial regulation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/gigascience\/giaf150","type":"journal-article","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T12:35:48Z","timestamp":1765197348000},"source":"Crossref","is-referenced-by-count":1,"title":["A sulfatide-centered ultra-high-resolution magnetic resonance MALDI imaging benchmark dataset for MS1-based lipid annotation tools"],"prefix":"10.1093","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9955-1550","authenticated-orcid":false,"given":"Lars","family":"Gruber","sequence":"first","affiliation":[{"name":"CeMOS Research and Transfer Center, Mass Spectrometry and Optical Spectroscopy, Technische Hochschule Mannheim , 68165 Mannheim, Germany"},{"name":"Medical Faculty, Heidelberg University , 69117 Heidelberg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2896-3290","authenticated-orcid":false,"given":"Stefan","family":"Schmidt","sequence":"additional","affiliation":[{"name":"CeMOS Research and Transfer Center, Mass Spectrometry and Optical Spectroscopy, Technische Hochschule Mannheim , 68165 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1789-4090","authenticated-orcid":false,"given":"Thomas","family":"Enzlein","sequence":"additional","affiliation":[{"name":"CeMOS Research and Transfer Center, Mass Spectrometry and Optical Spectroscopy, Technische Hochschule Mannheim , 68165 Mannheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0802-6451","authenticated-orcid":false,"given":"Carsten","family":"Hopf","sequence":"additional","affiliation":[{"name":"CeMOS Research and Transfer Center, Mass Spectrometry and Optical Spectroscopy, Technische Hochschule Mannheim , 68165 Mannheim, Germany"},{"name":"Medical Faculty, Heidelberg University , 69117 Heidelberg ,","place":["Germany"]},{"name":"Mannheim Center for Translational Neuroscience (MCTN), Medical Faculty Mannheim, Heidelberg University , 68167 Mannheim ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,12,9]]},"reference":[{"key":"2026010506562965700_bib1","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1146\/annurev-biodatasci-011420-031537","article-title":"Spatial metabolomics and imaging mass spectrometry in the age of artificial intelligence","volume":"3","author":"Alexandrov","year":"2020","journal-title":"Annu Rev Biomed Data Sci"},{"key":"2026010506562965700_bib2","doi-asserted-by":"publisher","first-page":"1927","DOI":"10.1002\/mas.21794","article-title":"What are we imaging? 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