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To address these issues, CamK-DB was developed as a reference-free Camellia fingerprinting database built on MIKE MinHash sketches. We curated 418 candidate resequencing datasets, and built a database using standardized 5\u00d7 genome-coverage fingerprints. Each accession is stored as a MIKE. jac fingerprint generated with k = 21 and recommended sketch\/pre_cnt = 2000. CamK-DB provides a command-line interface for data management and a custom C++ query engine that computes top-10 matches using Jaccard similarity, complemented by a QT-based graphical interface for interactive analysis. This resource offers a robust and scalable framework for precise and routine germplasm identification, genomic phylogenetic inference, and strategic breeding program design. CamK-DB (database and code) is publicly available at\u00a0https:\/\/github.com\/sc-zhang\/CamK-DB. CamK-DB binaries are provided for Windows 10\/11 and Linux (x86_64, glibc \u2265 2.27).<\/jats:p>","DOI":"10.1093\/gigascience\/giag088","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:50:56Z","timestamp":1787313056000},"source":"Crossref","is-referenced-by-count":0,"title":["Cam\n                    <i>K<\/i>\n                    -DB: A\n                    <i>k<\/i>\n                    -mer MinHash fingerprint database for reference-free genotyping of\n                    <i>Camellia<\/i>\n                    accessions"],"prefix":"10.1093","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1337-4052","authenticated-orcid":false,"given":"Feiquan","family":"Wang","sequence":"first","affiliation":[{"name":"College of Tea and Food Sciences, Collaborative Innovation Center of Chinese Oolong Tea Industry, Wuyi University, Tea Engineering Research Center of Fujian Higher Education , Wuyishan Fujian 354300 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Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences , Shenzhen 518120 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2560-7143","authenticated-orcid":false,"given":"Yutao","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Tea and Food Sciences, Collaborative Innovation Center of Chinese Oolong Tea Industry, Wuyi University, Tea Engineering Research Center of Fujian Higher Education , Wuyishan Fujian 354300 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4854-3512","authenticated-orcid":false,"given":"Hua","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Tea and Food Sciences, Collaborative Innovation Center of Chinese Oolong Tea Industry, Wuyi University, Tea Engineering Research Center of Fujian Higher Education , Wuyishan Fujian 354300 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7777-5373","authenticated-orcid":false,"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Tea and Food Sciences, Collaborative Innovation Center of Chinese Oolong Tea Industry, Wuyi University, Tea Engineering Research Center of Fujian Higher Education , Wuyishan Fujian 354300 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5207-0882","authenticated-orcid":false,"given":"Xingtan","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tropical Crop Breeding, Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences , Shenzhen 518120 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