{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:26:11Z","timestamp":1769214371212,"version":"3.49.0"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,8,17]],"date-time":"2020-08-17T00:00:00Z","timestamp":1597622400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100008376","name":"Helsinki University Central Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008376","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Pseudomyxoma peritonei (PMP) is a highly mucinous adenocarcinoma growing in the peritoneal cavity and most commonly originating from the appendix. Glycans play an important role in carcinogenesis, and glycosylation is altered in malignant diseases, including PMP. We have previously demonstrated that fucosylation of N-glycans is increased in PMP, but we did not observe modulation of overall sialylation. As sialic acids can be attached to the rest of the glycan via \u03b12,3- or \u03b12,6-linkage, we have now analyzed the linkage patterns of sialic acids in tissue specimens of normal appendices, low-grade appendiceal mucinous neoplasms (LAMN), low-grade (LG) PMP and high-grade (HG) PMP. For the linkage analysis, the enzymatically released acidic N-glycans were first treated with ethyl esterification or \u03b12,3-sialidase digestion followed by MALDI-TOF mass spectrometry. Significant increase in the relative abundance of \u03b12,6-sialylated and decrease in \u03b12,3-sialylated N-glycans was observed in PMP tumors as compared to the normal appendices (P\u00a0&amp;lt;\u00a00.025). More specifically, increased \u03b12,6-sialylation (P\u00a0&amp;lt;\u00a00.05) and decreased \u03b12,3-sialylation (P\u00a0&amp;lt;\u00a00.01) were detected in afucosylated and monofucosylated N-glycans of PMPs, whereas the less abundant multifucosylated glycans, containing terminal fucose, demonstrated increased \u03b12,3-sialylation (P\u00a0&amp;lt;\u00a00.01). Importantly, the increase in \u03b12,6-sialylation was also detected between PMP and the appendiceal precursor lesion LAMN (P\u00a0&amp;lt;\u00a00.01). The identified glycosylation alterations produce ligands for sialic acid-binding immunoglobulin-like lectins (Siglecs) and sialofucosylated glycans binding selectins, which play a role in the peritoneal dissemination and progression of the disease.<\/jats:p>","DOI":"10.1093\/glycob\/cwaa079","type":"journal-article","created":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T11:23:04Z","timestamp":1597317784000},"page":"211-222","source":"Crossref","is-referenced-by-count":6,"title":["Altered linkage pattern of N-glycan sialic acids in pseudomyxoma peritonei"],"prefix":"10.1093","volume":"31","author":[{"given":"Pirjo","family":"Nummela","sequence":"first","affiliation":[{"name":"Applied Tumor Genomics Research Program, Research Programs Unit, Faculty of Medicine, University of Helsinki, Haartmaninkatu 8, FI-00290, Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annamari","family":"Heiskanen","sequence":"additional","affiliation":[{"name":"Glykos 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Stenb\u00e4ckinkatu 9A, FI-00290 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tero","family":"Satomaa","sequence":"additional","affiliation":[{"name":"Glykos Finland Ltd, Viikinkaari 6, FI-00790 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ari","family":"Ristim\u00e4ki","sequence":"additional","affiliation":[{"name":"Applied Tumor Genomics Research Program, Research Programs Unit, Faculty of Medicine, University of Helsinki, Haartmaninkatu 8, FI-00290, Helsinki, Finland"},{"name":"Department of Pathology, HUSLAB, University of Helsinki and Helsinki University Hospital, Haartmaninkatu 3, FI-00290 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,8,17]]},"reference":[{"key":"2021040613075456900_ref1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/gm559","article-title":"Genome-wide mutational landscape of mucinous 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