{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T23:09:07Z","timestamp":1770419347686,"version":"3.49.0"},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T00:00:00Z","timestamp":1599177600000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/V011324\/1"],"award-info":[{"award-number":["BB\/V011324\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The novel coronavirus SARS-CoV-2, the infective agent causing COVID-19, is having a global impact both in terms of human disease as well as socially and economically. Its heavily glycosylated spike glycoprotein is fundamental for the infection process, via its receptor-binding domains interaction with the glycoprotein angiotensin-converting enzyme 2 on human cell surfaces. We therefore utilized an integrated glycomic and glycoproteomic analytical strategy to characterize both N- and O- glycan site-specific glycosylation within the receptor-binding domain. We demonstrate the presence of complex-type N-glycans with unusual fucosylated LacdiNAc at both sites N331 and N343 and a single site of O-glycosylation on T323.<\/jats:p>","DOI":"10.1093\/glycob\/cwaa085","type":"journal-article","created":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T11:09:44Z","timestamp":1598440184000},"page":"181-187","source":"Crossref","is-referenced-by-count":48,"title":["Site-specific characterization of SARS-CoV-2 spike glycoprotein receptor-binding domain"],"prefix":"10.1093","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1592-4092","authenticated-orcid":false,"given":"Aristotelis","family":"Antonopoulos","sequence":"first","affiliation":[{"name":"Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Broome","sequence":"additional","affiliation":[{"name":"BioPharmaSpec Inc, 363 Phoenixville Pike, Malvern, PA 19355, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Sharov","sequence":"additional","affiliation":[{"name":"BioPharmaSpec Inc, 363 Phoenixville Pike, Malvern, PA 19355, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Ziegenfuss","sequence":"additional","affiliation":[{"name":"BioPharmaSpec Inc, 363 Phoenixville Pike, Malvern, PA 19355, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard L","family":"Easton","sequence":"additional","affiliation":[{"name":"BioPharmaSpec Ltd, Suite 3.1, Lido Medical Centre, St. Saviour, Jersey, JE2 7LA, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Panico","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK"},{"name":"BioPharmaSpec Inc, 363 Phoenixville Pike, Malvern, PA 19355, USA"},{"name":"BioPharmaSpec Ltd, Suite 3.1, Lido Medical Centre, St. Saviour, Jersey, JE2 7LA, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Dell","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Howard R","family":"Morris","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK"},{"name":"BioPharmaSpec Inc, 363 Phoenixville Pike, Malvern, PA 19355, USA"},{"name":"BioPharmaSpec Ltd, Suite 3.1, Lido Medical Centre, St. Saviour, Jersey, JE2 7LA, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stuart M","family":"Haslam","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, London, SW7 2AZ, 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