{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T07:12:19Z","timestamp":1760425939576,"version":"3.37.3"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T00:00:00Z","timestamp":1614038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Deutsche Forschungsgemeinschaft in the SPP 2127","award":["HA 6014\/5-1","DFG DE 719\/7-1"],"award-info":[{"award-number":["HA 6014\/5-1","DFG DE 719\/7-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,8,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Desialylation of cell surface glycoproteins carried out by sialidases affects various immunological processes. However, the role of neuraminidase 1 (NEU1), one of the four mammalian sialidases, in inflammation and autoimmune disease is not completely unraveled to date. In this study, we analyzed the retinal expression of NEU1 in equine recurrent uveitis (ERU), a spontaneous animal model for autoimmune uveitis. Mass spectrometry revealed significantly higher abundance of NEU1 in retinal M\u00fcller glial cells (RMG) of ERU-diseased horses compared to healthy controls. Immunohistochemistry uncovered NEU1 expression along the whole M\u00fcller cell body in healthy and uveitic states and confirmed higher abundance in inflamed retina. M\u00fcller glial cells are the principal macroglial cells of the retina and play a crucial role in uveitis pathogenesis. To determine whether higher expression levels of NEU1 in uveitic RMG correlate with the desialylation of retinal cells, we performed lectin-binding assays with sialic acid-specific lectins. Through these experiments, we could demonstrate a profound loss of both \u03b12-3- and \u03b12-6-linked terminal sialic acids in uveitis. Hence, we hypothesize that the higher abundance of NEU1 in uveitic RMG plays an important role in the pathogenesis of uveitis by desialylation of retinal cells. As RMG become activated in the course of uveitis and actively promote inflammation, we propose that NEU1 might represent a novel activation marker for inflammatory RMG. Our data provide novel insights in the expression and implication of NEU1 in inflammation and autoimmune disease.<\/jats:p>","DOI":"10.1093\/glycob\/cwab014","type":"journal-article","created":{"date-parts":[[2021,2,6]],"date-time":"2021-02-06T12:12:50Z","timestamp":1612613570000},"page":"873-883","source":"Crossref","is-referenced-by-count":11,"title":["NEU1 is more abundant in uveitic retina with concomitant desialylation of retinal cells"],"prefix":"10.1093","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8583-9558","authenticated-orcid":false,"given":"Lea","family":"Lorenz","sequence":"first","affiliation":[{"name":"Chair of Physiology, Department of Veterinary Sciences, LMU Munich, Martinsried 82152, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Amann","sequence":"additional","affiliation":[{"name":"Chair of Physiology, Department of Veterinary Sciences, LMU Munich, Martinsried 82152, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sieglinde","family":"Hirmer","sequence":"additional","affiliation":[{"name":"Chair of Physiology, Department of Veterinary Sciences, LMU Munich, Martinsried 82152, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roxane L","family":"Degroote","sequence":"additional","affiliation":[{"name":"Chair of Physiology, Department of Veterinary Sciences, LMU Munich, Martinsried 82152, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie M","family":"Hauck","sequence":"additional","affiliation":[{"name":"Research Unit Protein Science, Helmholtz Center Munich, German Research Center for Environmental Health (GmbH), Munich 80939, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornelia A","family":"Deeg","sequence":"additional","affiliation":[{"name":"Chair of Physiology, Department of Veterinary Sciences, LMU Munich, Martinsried 82152, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,2,23]]},"reference":[{"key":"2021080913014283600_ref1","doi-asserted-by":"crossref","DOI":"10.1111\/jnc.15024","article-title":"Lipopolysaccharide activates microglia via neuraminidase 1 desialylation of Toll-like Receptor 4","author":"Allendorf","year":"2020","journal-title":"J Neurochem"},{"key":"2021080913014283600_ref2","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1002\/glia.23757","article-title":"Activated microglia desialylate their surface, stimulating complement receptor 3-mediated phagocytosis of neurons","volume":"68","author":"Allendorf","year":"2020","journal-title":"Glia"},{"key":"2021080913014283600_ref3","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.cellsig.2009.09.038","article-title":"Neu 1 desialylation of sialyl \u03b1-2, 3-linked \u03b2-galactosyl residues of TOLL-like receptor 4 is essential for receptor activation and cellular signaling","volume":"22","author":"Amith","year":"2010","journal-title":"Cell Signal"},{"key":"2021080913014283600_ref4","doi-asserted-by":"crossref","first-page":"3156","DOI":"10.1101\/gad.10.24.3156","article-title":"Characterization of human lysosomal neuraminidase defines the molecular basis of the metabolic storage disorder sialidosis","volume":"10","author":"Bonten","year":"1996","journal-title":"Genes Dev"},{"key":"2021080913014283600_ref5","doi-asserted-by":"crossref","first-page":"37657","DOI":"10.1074\/jbc.M007380200","article-title":"Lysosomal neuraminidase. 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