{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:13:39Z","timestamp":1787066019711,"version":"build-2736575974"},"reference-count":130,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2021,4,7]],"date-time":"2021-04-07T00:00:00Z","timestamp":1617753600000},"content-version":"vor","delay-in-days":2,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01 AI114635"],"award-info":[{"award-number":["R01 AI114635"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000861","name":"Burroughs Wellcome Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000861","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Bacterial vaginosis (BV) is a condition of the vaginal microbiome in which there are few lactobacilli and abundant anaerobic bacteria. Members of the genus Gardnerella are often one of the most abundant bacteria in BV. BV is associated with a wide variety of poor health outcomes for women. It has been recognized since the 1980s that women with BV have detectable and sometimes markedly elevated levels of sialidase activity in vaginal fluids and that bacteria associated with this condition produce this activity in culture. Mounting evidence collected using diverse methodologies points to the conclusion that BV is associated with a reduction in intact sialoglycans in cervicovaginal secretions. Here we review evidence for the contributions of vaginal bacteria, especially Gardnerella, in the processes of mucosal sialoglycan degradation, uptake, metabolism and depletion. Our understanding of the impacts of vaginal sialoglycan degradation is still limited. However, the potential implications of sialic acid depletion are discussed in light of our current understanding of the roles played by sialoglycans in vaginal physiology.<\/jats:p>","DOI":"10.1093\/glycob\/cwab024","type":"journal-article","created":{"date-parts":[[2021,4,7]],"date-time":"2021-04-07T15:59:51Z","timestamp":1617811191000},"page":"667-680","source":"Crossref","is-referenced-by-count":35,"title":["Vaginal sialoglycan foraging by\n                    <i>Gardnerella vaginalis<\/i>\n                    : mucus barriers as a meal for unwelcome guests?"],"prefix":"10.1093","volume":"31","author":[{"given":"Kavita","family":"Agarwal","sequence":"first","affiliation":[{"name":"Department of Obstetrics , Gynecology and Reproductive Sciences, Glycobiology Research and Training Center, , 9500 Gilman Drive, La Jolla CA 92093,","place":["USA"]},{"name":"University of California San Diego , Gynecology and Reproductive Sciences, Glycobiology Research and Training Center, , 9500 Gilman Drive, La Jolla CA 92093,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda L","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Obstetrics , Gynecology and Reproductive Sciences, Glycobiology Research and Training Center, , 9500 Gilman Drive, La Jolla CA 92093,","place":["USA"]},{"name":"University of California San Diego , Gynecology and Reproductive Sciences, Glycobiology Research and Training Center, , 9500 Gilman Drive, La Jolla CA 92093,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,4,5]]},"reference":[{"key":"2026041420025687700_ref1","doi-asserted-by":"crossref","first-page":"e3000788","DOI":"10.1371\/journal.pbio.3000788","article-title":"Glycan cross-feeding supports mutualism between Fusobacterium and the vaginal microbiota","volume":"18","author":"Agarwal","year":"2020","journal-title":"PLoS Biol"},{"key":"2026041420025687700_ref2","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1084\/jem.20131853","article-title":"Siglec-5 and Siglec-14 are polymorphic paired receptors that modulate neutrophil and amnion signaling responses to group B Streptococcus","volume":"211","author":"Ali","year":"2014","journal-title":"J Exp Med"},{"key":"2026041420025687700_ref3","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/0002-9343(83)91112-9","article-title":"Nonspecific vaginitis. 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The vaginal infections and prematurity study group","volume":"333","author":"Hillier","year":"1995","journal-title":"N Engl J Med"},{"key":"2026041420025687700_ref46","first-page":"211","article-title":"Vaginal indicators of amniotic fluid infection in preterm labor","volume":"97","author":"Hitti","year":"2001","journal-title":"Obstet Gynecol"},{"key":"2026041420025687700_ref47","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1128\/jcm.32.1.176-186.1994","article-title":"Bacterial vaginosis and vaginal microorganisms in idiopathic premature labor and association with pregnancy outcome","volume":"32","author":"Holst","year":"1994","journal-title":"J Clin Microbiol"},{"key":"2026041420025687700_ref48","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1258\/0956462991914438","article-title":"Mucinase and sialidase activity of the vaginal microflora: Implications for the pathogenesis of preterm labour","volume":"10","author":"Howe","year":"1999","journal-title":"Int J STD 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