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Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins. We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A\/T\/N, serving as a normal bladder cell control. Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Gal\u03b21\u20134(Fuc\u03b11\u20133)GlcNAc\u03b21-R), while normal bladder epithelial A\/T\/N cells lack Lex expression. T24 and TCCSUP cells also lack Lex, whereas J82COT cells express low levels of Lex. Glycomics analyses revealed other major differences in fucosylation and sialylation of N-glycans between these cell types. O-glycans are highly differentiated, as RT4 cells synthesize core 2-based O-glycans that are lacking in the T24 cells. These differences in glycan expression correlated with differences in RNA expression levels of their cognate glycosyltransferases, including \u03b11\u20133\/4-fucosyltransferase genes. These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.<\/jats:p>","DOI":"10.1093\/glycob\/cwab083","type":"journal-article","created":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T15:14:14Z","timestamp":1627571654000},"page":"1444-1463","source":"Crossref","is-referenced-by-count":14,"title":["Major differences in glycosylation and fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines"],"prefix":"10.1093","volume":"31","author":[{"given":"Bernadette","family":"Ezeabikwa","sequence":"first","affiliation":[{"name":"Department of Natural Sciences, University of Maryland Eastern Shore, Princess Anne, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9508-3280","authenticated-orcid":false,"given":"Nandini","family":"Mondal","sequence":"additional","affiliation":[{"name":"Department of Surgery, Beth Israel Deaconess Medical Center\u2014Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1592-4092","authenticated-orcid":false,"given":"Aristotelis","family":"Antonopoulos","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, London SW7 2AZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart M","family":"Haslam","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, London SW7 2AZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyuki","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Surgery, Beth Israel Deaconess Medical Center\u2014Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Martin-Caraballo","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland Eastern Shore, Princess Anne, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Lehoux","sequence":"additional","affiliation":[{"name":"Department of Surgery, Beth Israel Deaconess Medical Center\u2014Harvard Medical School, Boston, MA, USA"},{"name":"Novab Inc., Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Msano","family":"Mandalasi","sequence":"additional","affiliation":[{"name":"Department of Natural Sciences, University of Maryland Eastern Shore, Princess Anne, MD, USA"},{"name":"Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Ishaque","sequence":"additional","affiliation":[{"name":"Department of Natural Sciences, University of Maryland Eastern Shore, Princess Anne, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4987-3016","authenticated-orcid":false,"given":"Jamie","family":"Heimburg-Molinaro","sequence":"additional","affiliation":[{"name":"Department of Surgery, Beth Israel Deaconess Medical Center\u2014Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8918-5034","authenticated-orcid":false,"given":"Richard D","family":"Cummings","sequence":"additional","affiliation":[{"name":"Department of Surgery, Beth Israel Deaconess Medical Center\u2014Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony 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