{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T23:23:27Z","timestamp":1783466607796,"version":"3.55.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T00:00:00Z","timestamp":1628640000000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Research Council of Norway","doi-asserted-by":"publisher","award":["NFR 272338\/E50"],"award-info":[{"award-number":["NFR 272338\/E50"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-16-SUSN-0001"],"award-info":[{"award-number":["ANR-16-SUSN-0001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["16\/RD\/SusAn\/ERA-NET"],"award-info":[{"award-number":["16\/RD\/SusAn\/ERA-NET"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Cervical mucus plays an important role in female fertility, since it allows the entry of motile and morphological normal sperm while preventing the ascent of pathogens from the vagina. The function of cervical mucus is critically linked to its rheological properties that are in turn dictated by O-glycosylated proteins, called mucins. We aimed to characterize the O-glycan composition in the cervical mucus of six European ewe breeds with known differences in pregnancy rates following cervical\/vaginal artificial insemination with frozen\u2013thawed semen, which are due to reported differences in cervical sperm transport. These were Suffolk (low fertility) and Belclare (medium fertility) in Ireland, Ile de France and Romanov (both with medium fertility) in France, and Norwegian White Sheep (NWS) and Fur (both with high fertility) in Norway (n\u00a0=\u00a028\u201330 ewes\/breed). We identified 124 O-glycans, from which 51 were the major glycans with core 2 and fucosylated glycans as the most common structures. The use of exogenous hormones for synchronization did not affect the O-glycan composition in both high-fertility ewe breeds, but it did in the other four ewe breeds. There was a higher abundance of the sulfated glycan (Gal\u03b21\u20133[SO3-GlcNAc\u03b21\u20136]GalNAc), fucosylated glycan (GlcNAc\u03b21\u20133(Fuc\u03b11\u20132Gal\u03b21\u20133)GalNAc) and core 4 glycan (GlcNAc\u03b21\u20133[GlcNAc\u03b21\u20136]GalNAc) in the low-fertility Suffolk breed compared with NWS (high fertility). In addition, core 4 glycans were negatively correlated with mucus viscosity. This novel study has identified O-glycans that are important for cervical sperm transport and could have applications across a range of species including human.<\/jats:p>","DOI":"10.1093\/glycob\/cwab085","type":"journal-article","created":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T19:25:42Z","timestamp":1628537142000},"page":"23-35","source":"Crossref","is-referenced-by-count":23,"title":["Identification and characterization of <i>O<\/i>-linked glycans in cervical mucus as biomarkers of sperm transport: A novel sheep model"],"prefix":"10.1093","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9628-134X","authenticated-orcid":false,"given":"Laura","family":"Abril-Parre\u00f1o","sequence":"first","affiliation":[{"name":"Laboratory of Animal Reproduction, Department of Biological Sciences, School of Natural Sciences, Biomaterials Research Cluster, Bernal Institute, Faculty of Science and Engineering, University of Limerick, V94 T9PX Limerick, Ireland"},{"name":"Teagasc, Animal & Grassland Research and Innovation Centre, C15 PW93 Grange, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayden","family":"Wilkinson","sequence":"additional","affiliation":[{"name":"NIBRT GlycoScience Group, The National Institute for Bioprocessing Research and Training, Blackrock, A94 X099 Dublin, Ireland"},{"name":"C\u00daRAM, SFI Research Centre for Medical Devices, National University of Ireland, H91 W2TY Galway, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anette","family":"Krogen\u00e6s","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Production Animal Clinical Sciences, Norwegian University of Life Sciences, 5003 1432 \u00c5s, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack","family":"Morgan","sequence":"additional","affiliation":[{"name":"NIBRT GlycoScience Group, The National Institute for Bioprocessing Research and Training, Blackrock, A94 X099 Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary E","family":"Gallagher","sequence":"additional","affiliation":[{"name":"Veterinary Sciences Centre, University College Dublin, D04 W6F6 Belfield, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colm","family":"Reid","sequence":"additional","affiliation":[{"name":"Veterinary Sciences Centre, University College Dublin, D04 W6F6 Belfield, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Druart","sequence":"additional","affiliation":[{"name":"UMR-PRC, INRA-85, Universit\u00e9 de Tours, IFCE, Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Fair","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Reproduction, Department of Biological Sciences, School of Natural Sciences, Biomaterials Research Cluster, Bernal Institute, Faculty of Science and Engineering, University of Limerick, V94 T9PX Limerick, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radka","family":"Saldova","sequence":"additional","affiliation":[{"name":"NIBRT GlycoScience Group, The National Institute for Bioprocessing Research and Training, Blackrock, A94 X099 Dublin, Ireland"},{"name":"C\u00daRAM, SFI Research Centre for Medical Devices, National University of Ireland, H91 W2TY Galway, Ireland"},{"name":"School of Medicine, College of Health and Agricultural Science, University College Dublin, D07 A8NN Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,8,10]]},"reference":[{"key":"2022022608291072100_ref1","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.theriogenology.2020.10.038","article-title":"Ewe breed differences in cervical 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