{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T12:47:50Z","timestamp":1770727670268,"version":"3.49.0"},"reference-count":70,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T00:00:00Z","timestamp":1637107200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Propionibacterium acnes, though generally considered part of the normal flora of human skin, is an opportunistic pathogen associated with acne vulgaris as well as other diseases, including endocarditis, endophthalmitis and prosthetic joint infections. Its virulence potential is also supported by knowledge gained from its sequenced genome. Indeed, a vaccine targeting a putative cell wall-anchored P. acnes sialidase has been shown to suppress cytotoxicity and pro-inflammatory cytokine release induced by the organism, and is proposed as an alternative treatment for P. acnes-associated diseases. Here, we report the crystal structures of the surface sialidase and its complex with the transition-state mimic Neu5Ac2en. Our structural and kinetic analyses, together with insight from a glycan array screen, which probes subtle specificities of the sialidase for \u03b1-2,3-sialosides, provide a basis for the structure-based design of novel small-molecule therapeutics against P. acnes infections.<\/jats:p>","DOI":"10.1093\/glycob\/cwab094","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T19:10:16Z","timestamp":1634843416000},"page":"162-170","source":"Crossref","is-referenced-by-count":11,"title":["Crystal structure of the <i>Propionibacterium acnes<\/i> surface sialidase, a drug target for <i>P. acnes<\/i>-associated diseases"],"prefix":"10.1093","volume":"32","author":[{"given":"Angel C Y","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada"},{"name":"Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, 2350 Health Sciences Mall, V6T 1Z3, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gesa","family":"Volkers","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada"},{"name":"Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, 2350 Health Sciences Mall, V6T 1Z3, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seino A K","family":"Jongkees","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia, V6T 1Z1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liam J","family":"Worrall","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada"},{"name":"Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, 2350 Health Sciences Mall, V6T 1Z3, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen G","family":"Withers","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada"},{"name":"Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia, V6T 1Z1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalie C J","family":"Strynadka","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada"},{"name":"Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, 2350 Health Sciences Mall, V6T 1Z3, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,11,17]]},"reference":[{"key":"2022031916405838800_ref1","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1107\/S0907444902016657","article-title":"PHENIX: building new software for automated crystallographic structure determination","volume":"58","author":"Adams","year":"2002","journal-title":"Acta Crystallogr. 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