{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:46:36Z","timestamp":1792813596100,"version":"4.5.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T00:00:00Z","timestamp":1696377600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000874","name":"The Brain & Behavior Research Foundation","doi-asserted-by":"publisher","award":["30505"],"award-info":[{"award-number":["30505"]}],"id":[{"id":"10.13039\/100000874","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R24GM137763"],"award-info":[{"award-number":["R24GM137763"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The development and function of the brain requires N-linked glycosylation of proteins, which is a ubiquitous modification in the secretory pathway. N-glycans have a distinct composition and undergo tight regulation in the brain, but the spatial distribution of these structures remains relatively unexplored. Here, we systematically employed carbohydrate binding lectins with differing specificities to various classes of N-glycans and appropriate controls to identify glycan expression in multiple regions of the mouse brain. Lectins binding high-mannose-type N-glycans, the most abundant class of brain N-glycans, showed diffuse staining with some punctate structures observed on high magnification. Lectins binding specific motifs of complex N-glycans, including fucose and bisecting GlcNAc, showed more partitioned labeling, including to the synapse-rich molecular layer of the cerebellum. Understanding the spatial distribution of N-glycans across the brain will aid future studies of these critical protein modifications in development and disease of the brain.<\/jats:p>","DOI":"10.1093\/glycob\/cwad077","type":"journal-article","created":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T00:25:06Z","timestamp":1696033506000},"page":"935-942","source":"Crossref","is-referenced-by-count":13,"title":["N-glycans show distinct spatial distribution in mouse brain"],"prefix":"10.1093","volume":"33","author":[{"given":"Maxence","family":"Noel","sequence":"first","affiliation":[{"name":"Beth Israel Deaconess Medical Center, Harvard Medical School Department of Surgery, , 300 Brookline Ave, Boston, MA 02215 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard D","family":"Cummings","sequence":"additional","affiliation":[{"name":"Beth Israel Deaconess Medical Center, Harvard Medical School Department of Surgery, , 300 Brookline Ave, Boston, MA 02215 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert G","family":"Mealer","sequence":"additional","affiliation":[{"name":"Oregon Health & Science University Department of Psychiatry, , 3181 SW Sam Jackson Park Rd, Portland, OR 97239 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,10,4]]},"reference":[{"issue":"7","key":"2024021205295743000_ref1","doi-asserted-by":"crossref","first-page":"2400","DOI":"10.1016\/S0021-9258(17)30236-3","article-title":"Structural determinants of concanavalin a specificity for oligosaccharides","volume":"254","author":"Baenziger","year":"1979","journal-title":"J Biol Chem"},{"issue":"11","key":"2024021205295743000_ref2","doi-asserted-by":"crossref","first-page":"2993","DOI":"10.1021\/acschembio.1c00689","article-title":"A useful guide to lectin binding: machine-learning directed annotation of 57 unique lectin specificities","volume":"17","author":"Bojar","year":"2022","journal-title":"ACS Chem Biol"},{"issue":"4","key":"2024021205295743000_ref3","doi-asserted-by":"crossref","first-page":"112368","DOI":"10.1016\/j.celrep.2023.112368","article-title":"N-glycoproteomics of brain synapses and synaptic vesicles","volume":"42","author":"Bradberry","year":"2023","journal-title":"Cell Rep"},{"issue":"19","key":"2024021205295743000_ref4","doi-asserted-by":"crossref","first-page":"11230","DOI":"10.1016\/S0021-9258(18)33746-3","article-title":"Characterization of the structural determinants required for the high affinity interaction of asparagine-linked oligosaccharides with immobilized Phaseolus vulgaris leukoagglutinating and erythroagglutinating lectins","volume":"257","author":"Cummings","year":"1982","journal-title":"J Biol Chem"},{"key":"2024021205295743000_ref5","volume-title":"Essentials of Glycobiology","author":"Cummings","year":"2022","edition":"4th"},{"issue":"1","key":"2024021205295743000_ref6","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1146\/annurev-neuro-071714-034019","article-title":"Neurological aspects of human glycosylation disorders","volume":"38","author":"Freeze","year":"2015","journal-title":"Annu Rev Neurosci"},{"issue":"8","key":"2024021205295743000_ref7","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1093\/glycob\/cwr080","article-title":"Effective glycoanalysis with Maackia amurensis lectins requires a clear understanding of their binding specificities","volume":"21","author":"Geisler","year":"2011","journal-title":"Glycobiology"},{"key":"2024021205295743000_ref8","doi-asserted-by":"crossref","first-page":"e68910","DOI":"10.7554\/eLife.68910","article-title":"Activity-dependent Golgi satellite formation in dendrites reshapes the neuronal surface glycoproteome","volume":"10","author":"Govind","year":"2021","journal-title":"elife"},{"key":"2024021205295743000_ref9","doi-asserted-by":"crossref","first-page":"e20609","DOI":"10.7554\/eLife.20609","article-title":"Unconventional secretory processing diversifies neuronal ion channel properties","volume":"5","author":"Hanus","year":"2016","journal-title":"elife"},{"key":"2024021205295743000_ref10","doi-asserted-by":"crossref","first-page":"711955","DOI":"10.3389\/fnana.2021.711955","article-title":"Mass spectrometry imaging for glycome in the brain","volume":"15","author":"Hasan","year":"2021","journal-title":"Front Neuroanat"},{"key":"2024021205295743000_ref11","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/0076-6879(89)79135-7","article-title":"Snowdrop lectin","volume":"179","author":"Kaku","year":"1989","journal-title":"Methods Enzymol"},{"issue":"3","key":"2024021205295743000_ref12","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1007\/s10719-022-10055-x","article-title":"The dynamic brain N-glycome","volume":"39","author":"Klari\u0107","year":"2022","journal-title":"Glycoconj J"},{"issue":"7","key":"2024021205295743000_ref13","doi-asserted-by":"crossref","first-page":"2614","DOI":"10.1016\/S0021-9258(19)41645-1","article-title":"Interaction of immunoglobulin glycopeptides with concanavalin A","volume":"250","author":"Kornfeld","year":"1975","journal-title":"J Biol Chem"},{"issue":"42","key":"2024021205295743000_ref14","doi-asserted-by":"crossref","first-page":"11163","DOI":"10.1073\/pnas.1708319114","article-title":"Discovery of an O-mannosylation pathway selectively serving cadherins and protocadherins","volume":"114","author":"Larsen","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"46","key":"2024021205295743000_ref15","doi-asserted-by":"crossref","first-page":"28743","DOI":"10.1073\/pnas.2014207117","article-title":"Spatial and temporal diversity of glycome expression in mammalian brain","volume":"117","author":"Lee","year":"2020","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"2024021205295743000_ref16","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1039\/D0MO00044B","article-title":"N -glycosylation in isolated rat nerve terminals","volume":"17","author":"Matthies","year":"2021","journal-title":"Mol Omics"},{"issue":"3","key":"2024021205295743000_ref17","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1038\/s41380-022-01490-1","article-title":"The schizophrenia-associated variant in SLC39A8 alters protein glycosylation in the mouse brain","volume":"27","author":"Mealer","year":"2022","journal-title":"Mol Psychiatry"},{"issue":"14","key":"2024021205295743000_ref18","doi-asserted-by":"crossref","first-page":"5645","DOI":"10.1074\/jbc.M116.764357","article-title":"Rapid regulation of sialidase activity in response to neural activity and sialic acid removal during memory processing in rat hippocampus","volume":"292","author":"Minami","year":"2017","journal-title":"J Biol Chem"},{"issue":"1","key":"2024021205295743000_ref19","doi-asserted-by":"crossref","first-page":"100237","DOI":"10.1016\/j.xpro.2020.100237","article-title":"An optimized protocol for combined fluorescent lectin\/immunohistochemistry to characterize tissue-specific glycan distribution in human or rodent tissues","volume":"2","author":"Rebelo","year":"2021","journal-title":"STAR Protoc"},{"issue":"19","key":"2024021205295743000_ref20","doi-asserted-by":"crossref","first-page":"12842","DOI":"10.1021\/acs.analchem.0c01206","article-title":"Region-specific characterization of N-glycans in the striatum and substantia Nigra of an adult rodent brain","volume":"92","author":"Samal","year":"2020","journal-title":"Anal Chem"},{"issue":"3","key":"2024021205295743000_ref21","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1172\/JCI131564","article-title":"Prion protein glycans reduce intracerebral fibril formation and spongiosis in prion disease","volume":"130","author":"Sevillano","year":"2020","journal-title":"J Clin Invest"},{"issue":"4","key":"2024021205295743000_ref22","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1016\/S0021-9258(19)75677-4","article-title":"The elderberry (Sambucus nigra L.) bark lectin recognizes the Neu5Ac(alpha 2-6)Gal\/GalNAc sequence","volume":"262","author":"Shibuya","year":"1987","journal-title":"J Biol Chem"},{"issue":"12","key":"2024021205295743000_ref23","doi-asserted-by":"crossref","first-page":"100433","DOI":"10.1016\/j.mcpro.2022.100433","article-title":"Glycoproteomics landscape of asymptomatic and symptomatic human Alzheimer\u2019s disease brain","volume":"21","author":"Suttapitugsakul","year":"2022","journal-title":"Mol Cell Proteomics"},{"issue":"10","key":"2024021205295743000_ref24","doi-asserted-by":"crossref","first-page":"4576","DOI":"10.1016\/S0021-9258(18)68821-0","article-title":"The immobilized leukoagglutinin from the seeds of Maackia amurensis binds with high affinity to complex-type Asn-linked oligosaccharides containing terminal sialic acid-linked alpha-2,3 to penultimate galactose residues","volume":"263","author":"Wang","year":"1988","journal-title":"J Biol Chem"},{"issue":"1","key":"2024021205295743000_ref25","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1038\/s41467-021-27781-9","article-title":"Mammalian brain glycoproteins exhibit diminished glycan complexity compared to other tissues","volume":"13","author":"Williams","year":"2022","journal-title":"Nat Commun"},{"issue":"11","key":"2024021205295743000_ref26","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1016\/S0969-2126(96)00141-4","article-title":"The 2.0 a structure of a cross-linked complex between snowdrop lectin and a branched mannopentaose: evidence for two unique binding modes","volume":"4","author":"Wright","year":"1996","journal-title":"Structure"},{"issue":"8","key":"2024021205295743000_ref27","doi-asserted-by":"crossref","first-page":"4688","DOI":"10.1016\/S0021-9258(18)89125-6","article-title":"Fractionation of L-fucose-containing oligosaccharides on immobilized Aleuria aurantia lectin","volume":"260","author":"Yamashita","year":"1985","journal-title":"J Biol Chem"},{"issue":"1","key":"2024021205295743000_ref28","doi-asserted-by":"crossref","first-page":"43560","DOI":"10.1038\/srep43560","article-title":"A standardized method for lectin microarray-based tissue glycome mapping","volume":"7","author":"Zou","year":"2017","journal-title":"Sci Rep"}],"container-title":["Glycobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/glycob\/advance-article-pdf\/doi\/10.1093\/glycob\/cwad077\/56051078\/cwad077.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/glycob\/article-pdf\/33\/11\/935\/56660411\/cwad077.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/glycob\/article-pdf\/33\/11\/935\/56660411\/cwad077.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T00:31:41Z","timestamp":1707697901000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/glycob\/article\/33\/11\/935\/7288962"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,4]]},"references-count":28,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,10,4]]},"published-print":{"date-parts":[[2023,12,25]]}},"URL":"https:\/\/doi.org\/10.1093\/glycob\/cwad077","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.05.30.542954","asserted-by":"object"}]},"ISSN":["1460-2423"],"issn-type":[{"value":"1460-2423","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,11]]},"published":{"date-parts":[[2023,10,4]]}}}