{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T19:18:39Z","timestamp":1767899919130,"version":"3.49.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"vor","delay-in-days":11,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012538","name":"Swedish Cancer Foundation","doi-asserted-by":"publisher","award":["20 0759 PjF 01 H"],"award-info":[{"award-number":["20 0759 PjF 01 H"]}],"id":[{"id":"10.13039\/100012538","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","award":["2019-01152"],"award-info":[{"award-number":["2019-01152"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001862","name":"Swedish Research Council Formas","doi-asserted-by":"publisher","award":["2018-01419"],"award-info":[{"award-number":["2018-01419"]}],"id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Olle Engkvist Foundation"},{"name":"Carl Tryggers Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Infections pose a challenge for the fast growing aquaculture sector. Glycosphingolipids are cell membrane components that pathogens utilize for attachment to the host to initiate infection. Here, we characterized rainbow trout glycosphingolipids from five mucosal tissues using mass spectrometry and nuclear magnetic resonance and investigated binding of radiolabeled Aeromonas salmonicida to the glycosphingolipids on thin-layer chromatograms. 12 neutral and 14 acidic glycosphingolipids were identified. The glycosphingolipids isolated from the stomach and intestine were mainly neutral, whereas glycosphingolipids isolated from the skin, gills and pyloric caeca were largely acidic. Many of the acidic structures were poly-sialylated with shorter glycan structures in the skin compared to the other tissues. The sialic acids found were Neu5Ac and Neu5Gc. Most of the glycosphingolipids had isoglobo and ganglio core chains, or a combination of these. The epitopes on the rainbow trout glycosphingolipid glycans differed between epithelial sites leading to differences in pathogen binding. A major terminal epitope was fucose, that occurred attached to GalNAc in a \u03b11-3 linkage but also in the form of HexNAc-(Fuc-)HexNAc-R. A. salmonicida were shown to bind to neutral glycosphingolipids from the gill and intestine. This study is the first to do a comprehensive investigation of the rainbow trout glycosphingolipids and analyze binding of A. salmonicida to glycosphingolipids. The structural information paves the way for identification of ways of interfering in pathogen colonization processes to protect against infections in aquaculture and contributes towards understanding A. salmonicida infection mechanisms.<\/jats:p>","DOI":"10.1093\/glycob\/cwae055","type":"journal-article","created":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T04:56:26Z","timestamp":1723006586000},"source":"Crossref","is-referenced-by-count":1,"title":["Characterization of the rainbow trout (<i>Oncorhynchus mykiss<\/i>) mucosal glycosphingolipid repertoire and <i>Aeromonas salmonicida<\/i> binding to neutral glycosphingolipids"],"prefix":"10.1093","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8464-1211","authenticated-orcid":false,"given":"John","family":"Benktander","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg , Box 440, Medicinaregatan 9C, Gothenburg 405 30, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1459-5450","authenticated-orcid":false,"given":"Henrik","family":"Sundh","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences , Box 463, Medicinareg 7B, , Gothenburg 405 30, Sweden"},{"name":"University of Gothenburg , Box 463, Medicinareg 7B, , Gothenburg 405 30, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7643-2083","authenticated-orcid":false,"given":"Kristina","family":"Sundell","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences , Box 463, Medicinareg 7B, , Gothenburg 405 30, Sweden"},{"name":"University of Gothenburg , Box 463, Medicinareg 7B, , Gothenburg 405 30, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0531-2137","authenticated-orcid":false,"given":"Sinan","family":"Sharba","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg , Box 440, Medicinaregatan 9C, Gothenburg 405 30, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1957-9553","authenticated-orcid":false,"given":"Susann","family":"Teneberg","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg , Box 440, Medicinaregatan 9C, Gothenburg 405 30, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7751-9586","authenticated-orcid":false,"given":"Sara K","family":"Lind\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg , Box 440, Medicinaregatan 9C, Gothenburg 405 30, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,8,7]]},"reference":[{"issue":"7","key":"2024080704234434900_ref1","doi-asserted-by":"crossref","first-page":"e1004234","DOI":"10.1371\/journal.ppat.1004234","article-title":"A repetitive DNA element regulates expression of the helicobacter pylori sialic acid binding adhesin by a rheostat-like mechanism","volume":"10","author":"Aberg","year":"2014","journal-title":"PLoS Pathog"},{"key":"2024080704234434900_ref2","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/S0076-6879(06)17020-2","article-title":"Helicobacter pylori adhesion to 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