{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T07:08:26Z","timestamp":1775977706747,"version":"3.50.1"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"United Kingdom Research and Innovation Biotechnology and Biological Sciences Research Council","award":["BB\/V014137\/1"],"award-info":[{"award-number":["BB\/V014137\/1"]}]},{"name":"China Scholarship Commission","award":["201708060177"],"award-info":[{"award-number":["201708060177"]}]},{"name":"Stanford Synchrotron Radiation Lightsource"},{"DOI":"10.13039\/100011664","name":"SLAC National Accelerator Laboratory","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011664","id-type":"DOI","asserted-by":"publisher"}]},{"name":"U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences","award":["DE-AC02-76SF00515"],"award-info":[{"award-number":["DE-AC02-76SF00515"]}]},{"name":"Department of Energy Office of Biological and Environmental Research"},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Science","doi-asserted-by":"crossref","award":["P30GM133894"],"award-info":[{"award-number":["P30GM133894"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The sugar-binding receptors dectin-2 and blood dendritic cell antigen 2 (BDCA-2) bind oligosaccharide ligands through extracellular carbohydrate-recognition domains (CRDs) and initiate intracellular signaling through Fc receptor \u03b3 adapters (FcR\u03b3). Dectin-2 stimulates macrophages in response to pathogen binding while BDCA-2 modulates cytokine production in plasmacytoid dendritic cells. The oligomeric states of these receptors and the orientations of their CRDs have been investigated by analysis of a naturally occurring disulfide-bonded variant of BDCA-2 and by replacement of transmembrane domains with N-terminal dimerization domains to create extracellular domain dimers of both dectin-2 and BDCA-2. Analysis of these constructs, as well as previously described crystal structures of the CRDs from these proteins and a novel structure of an extended version of the extracellular domain of dectin-2, showed that there is only limited interaction of the CRDs in the dimers, but interactions can be stabilized by the presence of the neck region. The resulting orientation of sugar-binding sites in the dimers would favor crosslinking of multiple dimers by oligosaccharide ligands, causing clustering of FcR\u03b3 to initiate signaling.<\/jats:p>","DOI":"10.1093\/glycob\/cwae082","type":"journal-article","created":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T19:49:11Z","timestamp":1727984951000},"source":"Crossref","is-referenced-by-count":3,"title":["Interactions that define the arrangement of sugar-binding sites in BDCA-2 and dectin-2 dimers"],"prefix":"10.1093","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0722-182X","authenticated-orcid":false,"given":"Yu","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Life Sciences , Sir Ernst Chain Building, , Exhibition Road, London SW7 2AZ,","place":["United Kingdom"]},{"name":"Imperial College , Sir Ernst Chain Building, , Exhibition Road, London SW7 2AZ,","place":["United 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