{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:05:57Z","timestamp":1790651157974,"version":"4.1.0"},"reference-count":83,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P50 HD096957"],"award-info":[{"award-number":["P50 HD096957"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32-A1007309"],"award-info":[{"award-number":["T32-A1007309"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Boston University Sexual Medicine Pilot"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Bacterial vaginosis (BV), a dysbiosis of the vaginal microbiome, affects approximately 30% of women worldwide (up to 50% in some regions) and is associated with several adverse health outcomes including preterm birth and increased incidence of sexually transmitted infections (STIs). BV-associated bacteria such as Gardnerella vaginalis and Prevotella timonensis damage the vaginal mucosa through the activity of sialidase enzymes that remodel the epithelial glycocalyx and degrade mucin glycoproteins. This damage may contribute to adverse health outcomes. However, whether BV-associated glycolytic enzymes also damage sperm has not yet been determined. Here, we show that sialidase-mediated glycocalyx remodeling of human sperm increases sperm susceptibility to damage and adversely affects their function in vitro. Specifically, we report that sperm motility was not adversely affected by sialidase treatment, but desialylated human sperm demonstrate increased susceptibility to agglutination and complement-mediated cytotoxicity as well as impaired transit through cervical mucus. Our results demonstrate mechanisms by which BV-associated sialidases affect sperm survival and function and potentially contribute to adverse reproductive outcomes such as infertility.<\/jats:p>","DOI":"10.1093\/glycob\/cwaf067","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T13:20:31Z","timestamp":1761830431000},"source":"Crossref","is-referenced-by-count":5,"title":["Sialidases derived from\n                    <i>Gardnerella vaginalis<\/i>\n                    and\n                    <i>Prevotella timonensis<\/i>\n                    remodel the sperm glycocalyx and impair sperm function"],"prefix":"10.1093","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7003-0796","authenticated-orcid":false,"given":"Sarah","family":"Dohadwala","sequence":"first","affiliation":[{"name":"Department of Virology , Immunology and Microbiology, Boston University Chobanian & Avedisian School of Medicine, 670 Albany Street, Boston, MA 02118,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Purna","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Biology , Boston University, 5 Cummington Mall, Boston, MA 02215,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maura K","family":"Farrell","sequence":"additional","affiliation":[{"name":"Department of Human Physiology , Boston University, 35 Commonwealth Ave., Boston, MA 02215,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph A","family":"Politch","sequence":"additional","affiliation":[{"name":"Department of Medicine , Boston University Chobanian & Avedisian School of Medicine, 670 Albany Street, Boston, MA 02118,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jai","family":"Marathe","sequence":"additional","affiliation":[{"name":"Department of Medicine , Boston University Chobanian & Avedisian School of Medicine, 670 Albany Street, Boston, MA 02118,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine E","family":"Costello","sequence":"additional","affiliation":[{"name":"Center for Biomedical Mass Spectrometry , Department of Biochemistry and Cell Biology, Boston University Chobanian & Avedisian School of Medicine, 670 Albany Street, Boston, MA 02118,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah J","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Virology , Immunology and Microbiology, Boston University Chobanian & Avedisian School of Medicine, 670 Albany Street, Boston, MA 02118,","place":["USA"]},{"name":"Department of Medicine , Boston University Chobanian & Avedisian School of Medicine, 670 Albany Street, Boston, MA 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