{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:09:27Z","timestamp":1773655767436,"version":"3.50.1"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100004488","name":"Croatian Science Foundation","doi-asserted-by":"publisher","award":["UIP-2019-04-5692"],"award-info":[{"award-number":["UIP-2019-04-5692"]}],"id":[{"id":"10.13039\/501100004488","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cardiovascular diseases (CVD) are the leading cause of death globally, with coronary artery disease (CAD) as the most prevalent single cause. While previous studies, including our own, have highlighted the role of immunoglobulin G (IgG) glycosylation in CAD, the present study is the first to investigate the total plasma protein N-glycome as a potential biomarker of CAD. In this cross-sectional study, we analyzed total plasma protein N-glycans from three independent study populations using hydrophilic interaction ultra-high performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLR). Meta-analysis revealed significant glycan alterations in individuals with coronary atherosclerosis compared with individuals without CAD. Specifically, highly branched, complex N-glycans (triantennary and sialylated glycans) were significantly increased in CAD+ cases (Effect\u00a0=\u00a00.193, SE\u00a0=\u00a00.0761, padj\u00a0=\u00a00.0358), whereas monogalactosylated (Effect\u00a0=\u00a0\u22120.2464, SE\u00a0=\u00a00.0768, padj\u00a0=\u00a00.00814) and core fucosylated glycans (Effect\u00a0=\u00a0\u22120.2025, SE\u00a0=\u00a00.0818, padj\u00a0=\u00a00.0358) were significantly decreased. These changes are consistent with an increased presence of acute-phase glycoproteins and systemic inflammation in CAD+ individuals. Our findings demonstrate that CAD-associated glycomic alterations are not confined to IgG but are present across the total plasma protein N-glycome, providing a broader molecular view of disease-associated glycosylation changes. Future studies should explore the integration of plasma glycan signatures with other inflammatory and metabolic markers to refine CAD risk stratification strategies.<\/jats:p>","DOI":"10.1093\/glycob\/cwag018","type":"journal-article","created":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T12:41:34Z","timestamp":1772800894000},"source":"Crossref","is-referenced-by-count":0,"title":["N-glycan branching on plasma proteins: a sweet fingerprint of coronary artery disease"],"prefix":"10.1093","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3333-486X","authenticated-orcid":false,"given":"Barbara Radovani","family":"Trbojevi\u0107","sequence":"first","affiliation":[{"name":"Genos Glycoscience Research Laboratory , Borongajska cesta 83h, 10000, Zagreb ,","place":["Croatia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frano","family":"Vu\u010dkovi\u0107","sequence":"additional","affiliation":[{"name":"Genos Glycoscience Research Laboratory , Borongajska cesta 83h, 10000, Zagreb ,","place":["Croatia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aldo P","family":"Maggioni","sequence":"additional","affiliation":[{"name":"Heart Care Foundation and ANMCO Research Center , Via Alfonso la Marmora 36, 50121, Florence ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ele","family":"Ferrannini","sequence":"additional","affiliation":[{"name":"CNR Institute of Clinical Physiology , Via Giuseppe Moruzzi 1, 56124, Pisa ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3729-4650","authenticated-orcid":false,"given":"Roberto","family":"Latini","sequence":"additional","affiliation":[{"name":"Mario Negri Institute for Pharmacological Research , Via Mario Negri, 2, 20156, Milan ,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9634-9511","authenticated-orcid":false,"given":"Janko","family":"Szavits-Nossan","sequence":"additional","affiliation":[{"name":"Magdalena University Hospital for Cardiovascular Diseases, Ul. Ljudevita Gaja 2, 49217, Krapinske Toplice, Croatia"},{"name":"Faculty of Dental Medicine and Health Osijek, J.J. Strossmayer University of Osijek, Crkvena ul. 21, 31000, Osijek, Croatia"},{"name":"Faculty of Medicine Osijek, J.J. Strossmayer University of Osijek, Ul. Josipa Huttlera 4, 31000, Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandar","family":"Blivajs","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University Hospital Dubrava, Av. Gojka \u0160u\u0161ka 6, 10000, Zagreb, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor","family":"\u0160esto","sequence":"additional","affiliation":[{"name":"Magdalena University Hospital for Cardiovascular Diseases, Ul. Ljudevita Gaja 2, 49217, Krapinske Toplice, Croatia"},{"name":"Faculty of Dental Medicine and Health Osijek, J.J. Strossmayer University of Osijek, Crkvena ul. 21, 31000, Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kre\u0161imir","family":"\u0160tambuk","sequence":"additional","affiliation":[{"name":"Magdalena University Hospital for Cardiovascular Diseases, Ul. Ljudevita Gaja 2, 49217, Krapinske Toplice, Croatia"},{"name":"Faculty of Dental Medicine and Health Osijek, J.J. Strossmayer University of Osijek, Crkvena ul. 21, 31000, Osijek, Croatia"},{"name":"Faculty of Medicine Osijek, J.J. Strossmayer University of Osijek, Ul. Josipa Huttlera 4, 31000, Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lovorka","family":"\u00d0erek","sequence":"additional","affiliation":[{"name":"Clinical Department for Laboratory Diagnostics, University Hospital Dubrava, Av. 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