{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T12:43:34Z","timestamp":1776689014444,"version":"3.51.2"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>N-glycans are essential components of glycoproteins, influencing their properties and functions. While biochemical pathways of glycosylation are well-characterized, their genetic regulation remains poorly understood. This study utilizes matrix-assisted laser desorption\/ionization-mass spectrometry (MALDI-MS) and ultra-high performance liquid chromatography-fluorescence detection (UHPLC-FD) to strengthen replication and further characterize previously identified genome-wide association signals for the total human plasma N-glycome (TPNG). Univariate and multivariate genetic association meta-analyses involved 3385 samples across 143\u00a0N-glycome traits from the Hoorn Diabetes Care System and DiaGene cohorts as well as 3224 samples across 117\u00a0N-glycome traits from TwinsUK, CEDAR, QMDiab and SABRE cohorts. We successfully replicated ten previously identified but not replicated glycosylation quantitative trait loci (glyQTLs) and prioritized five high-confidence putative causal genes, including the glycosyltransferase MGAT4B and inflammation-related genes \u2013 C3 and FCGR2B. The linkage-specific sialic acid derivatization in MALDI-MS enabled delineation of genetic effects on \u03b12,3- and \u03b12,6-sialylation. Mass spectrometry analysis, triggered and guided by association to a locus containing B3GAT1 glucuronosyltransferase, provided evidence for hexuronic acid-containing glycans in human blood plasma. These findings advance our understanding of the genetic regulation of protein N-glycosylation and highlight the complementarity of different analytical approaches in glycomics research.<\/jats:p>","DOI":"10.1093\/glycob\/cwag029","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T11:52:32Z","timestamp":1776685952000},"source":"Crossref","is-referenced-by-count":0,"title":["Replication of 10 novel loci involved in human plasma protein N-glycosylation using MALDI-MS and UHPLC-FD data"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8529-4498","authenticated-orcid":false,"given":"Anna","family":"Timoshchuk","sequence":"first","affiliation":[{"name":"MSU Institute for Artificial Intelligence, Lomonosov Moscow State University , Moscow ,","place":["Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annemieke","family":"Naber","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Erasmus Medical Center, University Medical Center Rotterdam , Rotterdam ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0961-9152","authenticated-orcid":false,"given":"Roderick","family":"Slieker","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Data Science, Amsterdam Public Health Institute, Amsterdam Cardiovascular Sciences , Amsterdam ,","place":["the Netherlands"]},{"name":"Department of Cell and Chemical Biology, Leiden University Medical Center , Leiden ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Soplenkova","sequence":"additional","affiliation":[{"name":"MSU Institute for Artificial Intelligence, Lomonosov Moscow State University , Moscow ,","place":["Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"Maslov","sequence":"additional","affiliation":[{"name":"MSU Institute for Artificial Intelligence, Lomonosov Moscow State University , Moscow ,","place":["Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadezhda A","family":"Potapova","sequence":"additional","affiliation":[{"name":"Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences , Novosibirsk ,","place":["Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Nicolardi","sequence":"additional","affiliation":[{"name":"Center for Proteomics and Metabolomics, Leiden University Medical Center , Leiden ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P J M","family":"Elders","sequence":"additional","affiliation":[{"name":"Department of General Practice, Amsterdam UMC, Location Vrije Universiteit , Meibergdreef 15, Amsterdam 1105AZ ,","place":["the Netherlands"]},{"name":"Amsterdam Public Health Research Institute , Amsterdam ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric J G","family":"Sijbrands","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Erasmus Medical Center, University Medical Center Rotterdam , Rotterdam ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0279-4900","authenticated-orcid":false,"given":"Sodbo","family":"Sharapov","sequence":"additional","affiliation":[{"name":"MSU Institute for Artificial Intelligence, Lomonosov Moscow State University , Moscow ,","place":["Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leen M 't","family":"Hart","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Data Science, Amsterdam Public Health Institute, Amsterdam Cardiovascular Sciences , Amsterdam ,","place":["the Netherlands"]},{"name":"Department of Cell and Chemical Biology, Leiden University Medical Center , Leiden ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mandy","family":"Hoek","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Erasmus Medical Center, University Medical Center Rotterdam , Rotterdam ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0814-4995","authenticated-orcid":false,"given":"Manfred","family":"Wuhrer","sequence":"additional","affiliation":[{"name":"Center for Proteomics and Metabolomics, Leiden University Medical Center , Leiden ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yurii S","family":"Aulchenko","sequence":"additional","affiliation":[{"name":"MSU Institute for Artificial Intelligence, Lomonosov Moscow State University , Moscow ,","place":["Russia"]},{"name":"Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences , Novosibirsk ,","place":["Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2026,4,20]]},"container-title":["Glycobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/glycob\/advance-article-pdf\/doi\/10.1093\/glycob\/cwag029\/68115096\/cwag029.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/glycob\/advance-article-pdf\/doi\/10.1093\/glycob\/cwag029\/68115096\/cwag029.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T11:52:32Z","timestamp":1776685952000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/glycob\/advance-article\/doi\/10.1093\/glycob\/cwag029\/8659207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,20]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/glycob\/cwag029","relation":{},"ISSN":["1460-2423"],"issn-type":[{"value":"1460-2423","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,20]]},"article-number":"cwag029"}}