{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:05:13Z","timestamp":1792785913923,"version":"4.5.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"vor","delay-in-days":22,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JST-CREST","award":["JPMJCR21E3"],"award-info":[{"award-number":["JPMJCR21E3"]}]},{"name":"JST FOREST Program","award":["JPMJFR2255"],"award-info":[{"award-number":["JPMJFR2255"]}]},{"name":"JST SPRING","award":["JPMJSP2102"],"award-info":[{"award-number":["JPMJSP2102"]}]},{"name":"Human Glycome Atlas Project, MEXT\/JSPS KAKENHI","award":["JP25K02410"],"award-info":[{"award-number":["JP25K02410"]}]},{"name":"Human Glycome Atlas Project, MEXT\/JSPS KAKENHI","award":["JP20K21495"],"award-info":[{"award-number":["JP20K21495"]}]},{"name":"Human Glycome Atlas Project, MEXT\/JSPS KAKENHI","award":["JP24H00599"],"award-info":[{"award-number":["JP24H00599"]}]},{"name":"Joint Research by Exploratory Research Center on Life and Living Systems","award":["24EXC901"],"award-info":[{"award-number":["24EXC901"]}],"id":[{"id":"https:\/\/ror.org\/005t7z309","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Joint Research by Exploratory Research Center on Life and Living Systems","award":["25EXC603"],"award-info":[{"award-number":["25EXC603"]}],"id":[{"id":"https:\/\/ror.org\/005t7z309","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Joint Research by Exploratory Research Center on Life and Living Systems","award":["26EXC317"],"award-info":[{"award-number":["26EXC317"]}],"id":[{"id":"https:\/\/ror.org\/005t7z309","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Outstanding Research Group Support Program in Nagoya City University","award":["2401101"],"award-info":[{"award-number":["2401101"]}],"id":[{"id":"https:\/\/ror.org\/04wn7wc95","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Outstanding Research Group Support Program in Nagoya City University","award":["2520001"],"award-info":[{"award-number":["2520001"]}],"id":[{"id":"https:\/\/ror.org\/04wn7wc95","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Outstanding Research Group Support Program in Nagoya City University","award":["2530002"],"award-info":[{"award-number":["2530002"]}],"id":[{"id":"https:\/\/ror.org\/04wn7wc95","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Protein N-glycosylation generates diverse glycoforms that influence protein structure and function, yet the molecular rules governing site-specific glycan maturation remain incompletely understood. Accumulating evidence indicates that glycosylation outcomes are shaped by determinants embedded within glycoproteins themselves, beyond enzyme availability alone. Here, we investigate how neighboring glycans modulate site-specific N-glycan maturation in human Fc\u03b3 receptor III (Fc\u03b3RIII). Building on previous glycoproteomic studies showing restricted processing of the N45 glycan in Fc\u03b3RIII molecules, we combined reciprocal glycosylation-site mutagenesis with molecular dynamics simulations. Introduction of an N64 glycosylation sequon into Fc\u03b3RIIIa reduced N45 glycan maturation, whereas removal of the N64 sequon from Fc\u03b3RIIIb produced the reciprocal effect. Simulations using glycan models, analyzed primarily by contact maps and contact frequencies, showed that the N45 glycan dynamically contacts neighboring N64 and N169 glycans. These findings support a model in which the isoform-specific N64 glycan, in addition to the adjacent N169 glycan shared by both Fc\u03b3RIII isoforms, contributes to reduced N45 glycan maturation by reshaping the local glycan environment around N45.<\/jats:p>","DOI":"10.1093\/glycob\/cwag075","type":"journal-article","created":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T11:45:50Z","timestamp":1789213550000},"source":"Crossref","is-referenced-by-count":0,"title":["Dynamic inter-glycan interactions regulate site-specific\n                    <i>N<\/i>\n                    -glycan maturation in human Fc\u03b3 receptor III"],"prefix":"10.1093","volume":"36","author":[{"given":"Yue","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Materials Science, Japan Advanced Institute of Science and Technology , 1-1 Asahidai, Nomi 9231292 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9296-0225","authenticated-orcid":false,"given":"Hirokazu","family":"Yagi","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Nagoya City University , 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603 ,","place":["Japan"]},{"name":"Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences , 5-1 Higashiyama, Myodaiji-cho, Okazaki 444-8787 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masako","family":"Okina","sequence":"additional","affiliation":[{"name":"Graduate School of Medical Sciences, Nagoya City University , 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Graduate School of Medical Sciences, Nagoya City University , 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7187-9612","authenticated-orcid":false,"given":"Koichi","family":"Kato","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, Nagoya City University , 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603 ,","place":["Japan"]},{"name":"Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences , 5-1 Higashiyama, Myodaiji-cho, Okazaki 444-8787 ,","place":["Japan"]},{"name":"Institute for Molecular Science, National Institutes of Natural Sciences , 5-1 Higashiyama, Myodaiji-cho, Okazaki 444-8787 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1521-1153","authenticated-orcid":false,"given":"Takumi","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"School of Materials Science, Japan Advanced Institute of Science and Technology , 1-1 Asahidai, Nomi 9231292 ,","place":["Japan"]},{"name":"Graduate School of Pharmaceutical Sciences, Nagoya City University , 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603 ,","place":["Japan"]},{"name":"Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences , 5-1 Higashiyama, Myodaiji-cho, Okazaki 444-8787 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"issue":"24","key":"2026091610314768400_ref1","doi-asserted-by":"publisher","first-page":"5640","DOI":"10.1182\/blood-2012-01-380121","article-title":"Properties of mouse and human IgG receptors and their contribution to disease","volume":"119","author":"Bruhns","year":"2012","journal-title":"Blood"},{"issue":"8","key":"2026091610314768400_ref2","doi-asserted-by":"publisher","first-page":"5032","DOI":"10.1074\/jbc.M510171200","article-title":"The carbohydrate at Fc\u03b3RIIIa Asn-162. An element required for high affinity binding to non-fucosylated IgG glycoforms","volume":"281","author":"Ferrara","year":"2006","journal-title":"J Biol Chem"},{"issue":"31","key":"2026091610314768400_ref3","doi-asserted-by":"publisher","first-page":"12669","DOI":"10.1073\/pnas.1108455108","article-title":"Unique carbohydrate-carbohydrate interactions are required for high affinity binding between Fc\u03b3RIII and antibodies lacking core fucose","volume":"108","author":"Ferrara","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"10","key":"2026091610314768400_ref4","doi-asserted-by":"publisher","first-page":"2607","DOI":"10.1021\/acs.jpcb.1c00304","article-title":"Oligomannose N-glycans 3D architecture and its response to the Fc\u03b3RIIIa structural landscape","volume":"125","author":"Fogarty","year":"2021","journal-title":"J Phys Chem 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