{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:53:07Z","timestamp":1791557587570,"version":"4.3.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Since its discovery over forty-years ago, newly developed and improved existing technologies have played a crucial role in advancing O-GlcNAcylation research. We now know that O-GlcNAcylation is central to nutrient\/stress regulation of nearly all cellular processes. O-GlcNAcylation was discovered by using labeling by galactosyltransferase and tritiated UDP-GlcNAc combined with product analyses. This enzymatic probe remained the gold standard for early studies in the field. Subsequent development of O-GlcNAc specific antibodies greatly advanced the field by providing non-glycobiologists facile methods for identification of O-GlcNAcylated proteins. Purification and cloning of O-GlcNAc cycling enzymes allowed for development of specific inhibitors and for genetic studies to elucidate the functions of the sugar. Since O-GlcNAc is so labile in the gas phase and is subject to severe ion-suppression in classical collision-induced fragmentation, the development of electron transfer dissociation mass spectrometry was a major advance in O-GlcNAc glycoproteomics, leading to the development of large databases of O-GlcNAcylated proteins. Bioorthogonal chemistry provided valuable new tools to evaluate O-GlcNAc functions in living cells. These and other technological advances have played a major role during the past four decades in establishing O-GlcNAcylation as a major regulator of cellular metabolism.<\/jats:p>","DOI":"10.1093\/glycob\/cwag093","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T11:49:58Z","timestamp":1791460198000},"source":"Crossref","is-referenced-by-count":0,"title":["Milestones in O-GlcNAc Research: Technological Advances that Shaped the Field"],"prefix":"10.1093","author":[{"given":"John A","family":"Hanover","sequence":"first","affiliation":[{"name":"G. Gilbert Ashwell Distinguished Scientist, National Institute of Diabetes, Digestive and Kidney Diseases (NIDDK), National Institutes of Health"},{"name":"Chief, Laboratory of Cell and Molecular Biology, National Institute of Diabetes, Digestive and Kidney Diseases (NIDDK), National Institutes of Health"},{"name":"Director, Genomics Core, NIDDK, NIH"},{"name":"Chief, Cell Biochemistry Section, LCMB, NIDDK, NIH , Bldg 8 Rm B127"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7812-4351","authenticated-orcid":false,"given":"Gerald W","family":"Hart","sequence":"additional","affiliation":[{"name":"Emeritus) Georgia Research Alliance, William Henry Terry, Sr. Eminent Scholar in Drug Discovery, Professor of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia ( , Athens, GA 30602"},{"name":"Adjunct Professor, Department of Physiology, Johns Hopkins University, School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,9]]},"container-title":["Glycobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/glycob\/advance-article-pdf\/doi\/10.1093\/glycob\/cwag093\/71788172\/cwag093.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/glycob\/advance-article-pdf\/doi\/10.1093\/glycob\/cwag093\/71788172\/cwag093.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:25:41Z","timestamp":1791555941000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/glycob\/advance-article\/doi\/10.1093\/glycob\/cwag093\/8898645"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,9]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/glycob\/cwag093","relation":{},"ISSN":["1460-2423"],"issn-type":[{"value":"1460-2423","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,9]]},"article-number":"cwag093"}}