{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:53:07Z","timestamp":1791557587578,"version":"4.3.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Chemistry, through the synthesis of a radioactive sugar-donor, played a critical role in the discovery of O-GlcNAc and has been contributing indispensable tools ever since. Here, we cover where chemical methods have contributed to both the discovery and characterization of O-GlcNAc modifications. We will first discuss metabolic labeling and chemoenzymatic modification as two techniques that leverage the power of bioorthogonal chemistry to visualize, enrich, and even quantify O-GlcNAc modifications from living systems. We will then describe how solid-phase peptide synthesis can be combined with protein ligation methods as the only current pathway to homogeneously O-GlcNAc-modified proteins for downstream biological experiments. In each section, we point out the positives and considerations for each approach and provide protocols for normalization of best practices across the field.<\/jats:p>","DOI":"10.1093\/glycob\/cwag095","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T11:49:57Z","timestamp":1791460197000},"source":"Crossref","is-referenced-by-count":0,"title":["Chemical methods for O-GlcNAc detection and preparation of O-GlcNAc modified proteins"],"prefix":"10.1093","author":[{"given":"Rianna L","family":"Haynie-Cion","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Southern California , Los Angeles, CA 90089 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle Marie B","family":"Helmeke","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Southern California , Los Angeles, CA 90089 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongning","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Southern California , Los Angeles, CA 90089 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack T","family":"Swanson","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Southern California , Los Angeles, CA 90089 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3205-5615","authenticated-orcid":false,"given":"Matthew R","family":"Pratt","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Southern California , Los Angeles, CA 90089 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,9]]},"container-title":["Glycobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/glycob\/advance-article-pdf\/doi\/10.1093\/glycob\/cwag095\/71788175\/cwag095.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/glycob\/advance-article-pdf\/doi\/10.1093\/glycob\/cwag095\/71788175\/cwag095.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:25:41Z","timestamp":1791555941000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/glycob\/advance-article\/doi\/10.1093\/glycob\/cwag095\/8898646"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,9]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/glycob\/cwag095","relation":{},"ISSN":["1460-2423"],"issn-type":[{"value":"1460-2423","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,9]]},"article-number":"cwag095"}}