{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T12:34:41Z","timestamp":1793190881512,"version":"4.5.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"vor","delay-in-days":14,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Heparin is a sulfated glycosaminoglycan, and the most used pharmaceutical anticoagulant worldwide. Heparin is isolated primarily from porcine sources, and possesses a complex variable structure, wherein anticoagulant activity is principally derived from the pentasaccharide GlcNS6S-GlcA-GlcNS3S6S-IdoA2S-GlcNS6S (AGA*IA) motif, containing an infrequent 3-O-sulfated glucosamine that enhances antithrombin stabilization. Pedobacter heparinus heparin lyases are currently widely employed for characterization of heparin and its derivatives, but have limited activity near regions of 3-O-sulfation, hindering analyses of anticoagulation relevant sequences. Here, using a panel of biophysical and structural techniques, we show that Bacteroides eggerthii lyase I liberates \u0394UA-GlcNS3S6S disaccharides from the defined pentasaccharide, fondaparinux. This activity provides improved access to 3-O-sulfated heparin standards, with potential to aid heparin quality control.<\/jats:p>","DOI":"10.1093\/glycob\/cwag097","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T11:39:30Z","timestamp":1791891570000},"source":"Crossref","is-referenced-by-count":0,"title":["Functional characterization of a 3-O-sulfate tolerant heparin lyase from\n                    <i>Bacteroides eggerthii<\/i>"],"prefix":"10.1093","volume":"36","author":[{"given":"Taran K","family":"Kandola","sequence":"first","affiliation":[{"name":"Centre for Marine and Coastal Studies (CEMACS), Universiti Sains Malaysia , 11800 USM, Penang ,","place":["Malaysia"]},{"name":"Centre for Glycoscience Research and Training, Keele University , Newcastle-under-Lyme, Staffordshire, ST5 5BG ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney J","family":"Mycroft-West","sequence":"additional","affiliation":[{"name":"The Rosalind Franklin Institute, Harwell Science and Innovation Campus , OX11 0QX ,","place":["United Kingdom"]},{"name":"Department of Biochemistry, University of Oxford , South Parks Road, Oxford, OX1 3QU ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo A","family":"Lima","sequence":"additional","affiliation":[{"name":"Centre for Glycoscience Research and Training, Keele University , Newcastle-under-Lyme, Staffordshire, ST5 5BG ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigail","family":"Turner","sequence":"additional","affiliation":[{"name":"The Rosalind Franklin Institute, Harwell Science and Innovation Campus , OX11 0QX ,","place":["United Kingdom"]},{"name":"Department of Biochemistry, University of Oxford , South Parks Road, Oxford, OX1 3QU ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Gardini","sequence":"additional","affiliation":[{"name":"Centro Alta Tecnologia Istituto di Ricerche Chimiche e Biochimiche \u2018G. Ronzoni\u2019 S.r.l. , Via G. Colombo 81, 20133, Milan ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Urso","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Chimiche e Biochimiche \u2018G. Ronzoni\u2019 , Via G. Colombo 81, 20133, Milan ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A","family":"Torode","sequence":"additional","affiliation":[{"name":"Centre for Glycoscience Research and Training, Keele University , Newcastle-under-Lyme, Staffordshire , ST5 5BG,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gavin J","family":"Miller","sequence":"additional","affiliation":[{"name":"Centre for Glycoscience Research and Training, Keele University , Newcastle-under-Lyme, Staffordshire, ST5 5BG ,","place":["United Kingdom"]},{"name":"Manchester Institute of Biotechnology, University of Manchester , 131 Princess Street, Manchester, M1 7DN ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Bisio","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Chimiche e Biochimiche \u2018G. Ronzoni\u2019 , Via G. Colombo 81, 20133, Milan ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin A","family":"Yates","sequence":"additional","affiliation":[{"name":"Centre for Glycoscience Research and Training, Keele University , Newcastle-under-Lyme, Staffordshire, ST5 5BG ,","place":["United Kingdom"]},{"name":"Istituto di Ricerche Chimiche e Biochimiche \u2018G. Ronzoni\u2019 , Via G. Colombo 81, 20133, Milan ,","place":["Italy"]},{"name":"Department of Biochemistry, Cell and Systems Biology, Institute of Structural, Molecular and Integrative Biology, University of Liverpool , Crown Street, Liverpool , L69 7ZB,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Guerrini","sequence":"additional","affiliation":[{"name":"Istituto di Ricerche Chimiche e Biochimiche \u2018G. Ronzoni\u2019 , Via G. Colombo 81, 20133, Milan ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A","family":"Skidmore","sequence":"additional","affiliation":[{"name":"Centre for Marine and Coastal Studies (CEMACS), Universiti Sains Malaysia , 11800 USM, Penang ,","place":["Malaysia"]},{"name":"Centre for Glycoscience Research and Training, Keele University , Newcastle-under-Lyme, Staffordshire , ST5 5BG,","place":["United Kingdom"]},{"name":"Department of Biochemistry, Cell and Systems Biology, Institute of Structural, Molecular and Integrative Biology, University of Liverpool , Crown Street, Liverpool, L69 7ZB ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0294-7065","authenticated-orcid":false,"given":"Liang","family":"Wu","sequence":"additional","affiliation":[{"name":"The Rosalind Franklin Institute, Harwell Science and Innovation Campus , OX11 0QX ,","place":["United 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