{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T05:09:45Z","timestamp":1787893785799,"version":"build-2784847793"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2019,8,14]],"date-time":"2019-08-14T00:00:00Z","timestamp":1565740800000},"content-version":"vor","delay-in-days":76,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"European Structural and Investment Funds IRI","award":["KK.01.2.1.01.0003"],"award-info":[{"award-number":["KK.01.2.1.01.0003"]}]},{"name":"Croatian Centre of Research Excellence in Personalized Healthcare","award":["KK.01.1.1.01.0010"],"award-info":[{"award-number":["KK.01.1.1.01.0010"]}]},{"name":"European Commission through Horizon 2020 programme, Marie-Sk\u0142odowska Curie European Training Network","award":["676421"],"award-info":[{"award-number":["676421"]}]},{"name":"Ministry of Science and Education of the Republic of Croatia","award":["309\u20130061194-2023"],"award-info":[{"award-number":["309\u20130061194-2023"]}]},{"name":"European Commission through European Structural and Investment","award":["2014-2020"],"award-info":[{"award-number":["2014-2020"]}]},{"name":"European Commission through Horizon 2020"},{"DOI":"10.13039\/501100006588","name":"Ministry of Science, Education and Sports","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006588","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,11,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated from dried blood spots (DBSs), which was performed in a high-throughput mode. We compared the IgG N-glycan profiles originating from DBS with those originating from plasma, compared different media for DBS collection, evaluated analytical variation and assessed IgG N-glycan profile stability for different storage conditions. In conclusion, we show that DBSs are a good and stable source material for a robust IgG N-glycan analysis by ultra-performance liquid chromatography, suitable for blood sampling in conditions where no trained personnel and necessary laboratory equipment are available.<\/jats:p>","DOI":"10.1093\/glycob\/cwz061","type":"journal-article","created":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T15:12:21Z","timestamp":1565190741000},"page":"817-821","source":"Crossref","is-referenced-by-count":19,"title":["Comprehensive N-glycosylation analysis of immunoglobulin G from dried blood spots"],"prefix":"10.1093","volume":"29","author":[{"given":"Jelena","family":"Simunovic","sequence":"first","affiliation":[{"name":"Genos Glycoscience Research Laboratory, Borongajska cesta 83H, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marija","family":"Vilaj","sequence":"additional","affiliation":[{"name":"Genos Glycoscience Research Laboratory, Borongajska cesta 83H, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irena","family":"Trbojevic-Akmacic","sequence":"additional","affiliation":[{"name":"Genos Glycoscience Research Laboratory, Borongajska cesta 83H, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Momcilovic","sequence":"additional","affiliation":[{"name":"Genos Glycoscience Research Laboratory, Borongajska cesta 83H, 10000 Zagreb, Croatia"},{"name":"Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2333 ZA Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frano","family":"Vuckovic","sequence":"additional","affiliation":[{"name":"Genos Glycoscience Research Laboratory, Borongajska cesta 83H, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Gudelj","sequence":"additional","affiliation":[{"name":"Genos Glycoscience Research Laboratory, Borongajska cesta 83H, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julija","family":"Juric","sequence":"additional","affiliation":[{"name":"Genos Glycoscience Research Laboratory, Borongajska cesta 83H, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natali","family":"Nakic","sequence":"additional","affiliation":[{"name":"Laboratory of Prion Biology, Department of Neuroscience, Scuola Internazionale Superiore di Studi Avanzati, Via Bonomea 265, 34136 Trieste, Italy and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordan","family":"Lauc","sequence":"additional","affiliation":[{"name":"Genos Glycoscience Research Laboratory, Borongajska cesta 83H, 10000 Zagreb, Croatia"},{"name":"Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovacica 1, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6480-1427","authenticated-orcid":false,"given":"Marija","family":"Pezer","sequence":"additional","affiliation":[{"name":"Genos Glycoscience Research Laboratory, Borongajska cesta 83H, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,5,30]]},"reference":[{"key":"2019111902501962100_ref1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/978-1-4939-6493-2_17","article-title":"Automated integration of a UPLC glycomic profile","volume":"1503","author":"Agakova","year":"2017","journal-title":"Methods Mol Biol."},{"key":"2019111902501962100_ref2","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1074\/mcp.RA117.000015","article-title":"Stability of proteins in dried blood spot biobanks","volume":"16","author":"Bj\u00f6rkesten","year":"2017","journal-title":"Mol Cell 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