{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:02:26Z","timestamp":1753876946590,"version":"3.41.2"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T00:00:00Z","timestamp":1572998400000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Arizona Biomedical Research Council","award":["grant# 16-162513"],"award-info":[{"award-number":["grant# 16-162513"]}]},{"DOI":"10.13039\/100008227","name":"Achievement Rewards for College Scientists Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008227","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Coccidioides spp. are important pneumonia-causing pathogens of the American southwest, but little is known about their glycobiology and how their glycosylations differ from other pneumonia-causing fungi. There is mounting preliminary evidence to suggest genus or even species-specific glycosylations in the fungal kingdom due to the presence of unique Carbohydrate-Active Enzymes (CAZymes) in fungal genomes (Deshpande and others 2008; Karkowska-Kuleta and Kozik 2015). If Coccidioides spp.-specific glycans can be identified, it may be possible to exploit these differences to develop more specific diagnostic approaches and more effective therapeutics. Herein we i) mined Coccidioides spp. and other pathogenic fungal genomes to identify CAZymes specific for Coccidiodes spp., ii) proteomically determined the Coccidioides spp. \u201cCAZome\u201d produced in vivo and in vitro, and iii) utilized glycomics to differentiate Coccidioides genus-specific N-glycans from other pathogenic fungi. As far as we are aware, this is the first proteomic and glycomic comparison of the N-glycomes and CAZomes of different fungal genera during infection in human hosts.<\/jats:p>","DOI":"10.1093\/glycob\/cwz092","type":"journal-article","created":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T19:15:03Z","timestamp":1572030903000},"source":"Crossref","is-referenced-by-count":3,"title":["Carbo-loading in Coccidioides spp.: a quantitative analysis of CAZyme abundance and resulting glycan populations"],"prefix":"10.1093","author":[{"given":"Natalie M","family":"Mitchell","sequence":"first","affiliation":[{"name":"School of Life Sciences, Mayo Clinic Collaborative Research Building, Arizona State University, Scottsdale, AZ, 85259, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas E","family":"Grys","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic, Phoenix, AZ, 85054, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas F","family":"Lake","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Mayo Clinic Collaborative Research Building, Arizona State University, Scottsdale, AZ, 85259, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,11,6]]},"container-title":["Glycobiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/glycob\/advance-article-pdf\/doi\/10.1093\/glycob\/cwz092\/30419192\/cwz092.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/glycob\/advance-article-pdf\/doi\/10.1093\/glycob\/cwz092\/30419192\/cwz092.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T10:26:10Z","timestamp":1573035970000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/glycob\/advance-article\/doi\/10.1093\/glycob\/cwz092\/5613540"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,6]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/glycob\/cwz092","relation":{},"ISSN":["1460-2423"],"issn-type":[{"type":"electronic","value":"1460-2423"}],"subject":[],"published":{"date-parts":[[2019,11,6]]},"article-number":"cwz092"}}