{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T11:01:55Z","timestamp":1772362915259,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The thymus provides a crucial microenvironment for lymphocyte development, undergoes rapid growth by progressive atrophy, yet the underlying molecular dynamics remain largely unclear. Here, we present a comprehensive single-cell transcriptomic data resource of mouse thymus across four developmental stages (3, 9, 15, and 33 weeks). We identified eleven major immune cell types, including \u03b3\u03b4\u200bcells, B cells, NKT cells, Dendritic cells, immature single-positive-T cells, DN3a cells, double positive (DP)-T cells\u2013further subdivided into DP-1, DP-2, and DP-3\u2014as well as CD4+ and CD8+ T cells. Our analysis revealed dynamic remodeling of thymic cell composition and transcriptional profiles over time. Notably, the proportion of CD8+ T cells and the expression of Smad4 and Smad7, key regulators of lymphocyte development, declined during thymic involution. The spatial single-cell atlas revealed that DP-2 and DP-3 formed distinct clusters, with dendritic cells (DCs) located in closer proximity to DP-2. Notably, DC-DP-2 interactions were enriched via the MHC-II pathway, potentially promoting the differentiation of DP-T cells. Further spatial trajectory analysis delineated the developmental landscape of DP-2 cells. Finally, autoimmune disease\u2013associated risk genes were preferentially enriched in CD4+ and NKT cells, displaying distinct spatiotemporal expression patterns. Collectively, this study provides a valuable resource for dissecting thymic architecture and immune system development across different ages.<\/jats:p>","DOI":"10.1093\/gpbjnl\/qzag020","type":"journal-article","created":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T10:38:19Z","timestamp":1772361499000},"source":"Crossref","is-referenced-by-count":0,"title":["Spatial Single-cell Transcriptome Atlas of Mouse Thymus Reveals the T Lymphocyte Dynamics During Development"],"prefix":"10.1093","author":[{"given":"Jiahao","family":"Zhang","sequence":"first","affiliation":[{"name":"Huazhong Agricultural University State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, , Wuhan 430070,","place":["China"]},{"name":"South China Agricultural University Key Laboratory of Zoonoses, College of Veterinary Medicine, , Guangzhou 510642,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7668-7676","authenticated-orcid":false,"given":"Huaiyuan","family":"Cai","sequence":"additional","affiliation":[{"name":"Huazhong Agricultural University State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, , Wuhan 430070,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4231-5382","authenticated-orcid":false,"given":"Chuanyuan","family":"Lu","sequence":"additional","affiliation":[{"name":"Huazhong Agricultural University State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, , Wuhan 430070,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3837-399X","authenticated-orcid":false,"given":"Zhengze","family":"Wang","sequence":"additional","affiliation":[{"name":"Huazhong Agricultural University College of Life Science and Technology, , Wuhan 430070,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3903-3869","authenticated-orcid":false,"given":"Ruixuan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Huazhong Agricultural University State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, , Wuhan 430070,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6800-4954","authenticated-orcid":false,"given":"Lulu","family":"Deng","sequence":"additional","affiliation":[{"name":"Huazhong Agricultural University State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, , Wuhan 430070,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4117-3329","authenticated-orcid":false,"given":"Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Shenzhen University of Advanced Technology Faculty of Life and Health Sciences, , Shenzhen 518107,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2681-5924","authenticated-orcid":false,"given":"Wenbo","family":"He","sequence":"additional","affiliation":[{"name":"Huazhong Agricultural University State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, , Wuhan 430070,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Cao","sequence":"additional","affiliation":[{"name":"Shenzhen University of Advanced Technology Faculty of Life and Health Sciences, , Shenzhen 518107,","place":["China"]},{"name":"Chinese Academy of Sciences (CAS) The Brain Cognition and Brain Disease Institute of Shenzhen Institute of Advanced Technology, , Shenzhen 518107,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2026,3,1]]},"container-title":["Genomics, Proteomics &amp; Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/gpb\/advance-article-pdf\/doi\/10.1093\/gpbjnl\/qzag020\/67188360\/qzag020.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/gpb\/advance-article-pdf\/doi\/10.1093\/gpbjnl\/qzag020\/67188360\/qzag020.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T10:38:19Z","timestamp":1772361499000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/gpb\/advance-article\/doi\/10.1093\/gpbjnl\/qzag020\/8502574"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,1]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/gpbjnl\/qzag020","relation":{},"ISSN":["1672-0229","2210-3244"],"issn-type":[{"value":"1672-0229","type":"print"},{"value":"2210-3244","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,1]]},"article-number":"qzag020"}}