{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T06:59:40Z","timestamp":1781074780229,"version":"3.54.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"am","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Alternative polyadenylation (APA) is a key regulator of gene expression and cellular dynamics, yet systematic investigations of spatially resolved APA across diverse human tissues remain limited. Here, we developed SpatialAPA (https:\/\/github.com\/Omicslab-Zhang\/spatialAPA), a framework that benchmarks multiple APA identification methods and integrates spatial APA data with gene expression and cellular dynamics at spatial resolution. Applying SpatialAPA to 363 spatial transcriptomic datasets from 56 projects across 18 human tissues and 76 diseases, we constructed a spatially resolved APA atlas comprising 346,932 APA events across 52,175 genes. This atlas reveals organ-specific APA patterns and provides new insights into how APA regulates tissue homeostasis and disease progression beyond transcriptional control. To ensure cross-sample comparability, we applied batch correction, and spatial cell deconvolution was performed to uncover cell-type-specific dynamics and interactions. In triple-negative breast cancer, integrated spatial and single-cell analyses identified TSPAN8-positive epithelial subpopulations whose distinct APA regulation and transcriptional programs drive differentiation and malignant progression. To facilitate community access, we developed an online platform (http:\/\/www.biomedical-web.com\/spatialAPAdb\/home) for exploring APA, gene expression, and cellular dynamics in health and disease. Together, this study establishes the first comprehensive spatial APA atlas, providing a valuable resource and analytical framework for investigating molecular mechanisms and therapeutic targets.<\/jats:p>","DOI":"10.1093\/gpbjnl\/qzag042","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T06:26:25Z","timestamp":1781072785000},"source":"Crossref","is-referenced-by-count":0,"title":["A Spatially Resolved Atlas of Alternative Polyadenylation Across 18 Human Tissues and 76 Disease States"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8957-1761","authenticated-orcid":false,"given":"Zehang","family":"Jiang","sequence":"first","affiliation":[{"name":"Guangzhou Medical University The Key Laboratory of Advanced Interdisciplinary Studies, The First Affiliated Hospital of Guangzhou Medical University, GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, , Guangzhou 510182,","place":["China"]},{"name":"The First Affiliated Hospital of Guangzhou Medical University Department of Hepatobiliary Surgery, , Guangzhou 510120,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0017-6973","authenticated-orcid":false,"given":"Zhuochao","family":"Min","sequence":"additional","affiliation":[{"name":"Tel Aviv University School of Zoology, The George S. Wise Faculty of Life Sciences, , Tel Aviv 6997801,","place":["Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3020-1986","authenticated-orcid":false,"given":"Zhanying","family":"Wu","sequence":"additional","affiliation":[{"name":"Guangzhou Medical University The Key Laboratory of Advanced Interdisciplinary Studies, The First Affiliated Hospital of Guangzhou Medical University, GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, , Guangzhou 510182,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6729-7484","authenticated-orcid":false,"given":"Yubin","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangzhou Medical University The Key Laboratory of Advanced Interdisciplinary Studies, The First Affiliated Hospital of Guangzhou Medical University, GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, , Guangzhou 510182,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7348-5446","authenticated-orcid":false,"given":"Zhiyong","family":"Wu","sequence":"additional","affiliation":[{"name":"Guangzhou Medical University The Key Laboratory of Advanced Interdisciplinary Studies, The First Affiliated Hospital of Guangzhou Medical University, GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, , Guangzhou 510182,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0582-6124","authenticated-orcid":false,"given":"Huashu","family":"Wen","sequence":"additional","affiliation":[{"name":"General Hospital of Southern Theater Command of PLA , Guangzhou 510010,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8719-5004","authenticated-orcid":false,"given":"Cheng","family":"Wu","sequence":"additional","affiliation":[{"name":"Guangzhou Medical University The Key Laboratory of Advanced Interdisciplinary Studies, The First Affiliated Hospital of Guangzhou Medical University, GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, , Guangzhou 510182,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6572-9360","authenticated-orcid":false,"given":"Jia","family":"Guo","sequence":"additional","affiliation":[{"name":"Guangzhou Medical University The Key Laboratory of Advanced Interdisciplinary Studies, The First Affiliated Hospital of Guangzhou Medical University, GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, , Guangzhou 510182,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6687-3206","authenticated-orcid":false,"given":"Ke","family":"Si","sequence":"additional","affiliation":[{"name":"Guangzhou Medical University The Key Laboratory of Advanced Interdisciplinary Studies, The First Affiliated Hospital of Guangzhou Medical University, GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, , Guangzhou 510182,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8432-6164","authenticated-orcid":false,"given":"Douyue","family":"Li","sequence":"additional","affiliation":[{"name":"Guangzhou Medical University The Key Laboratory of Advanced Interdisciplinary Studies, The First Affiliated Hospital of Guangzhou Medical University, GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, , Guangzhou 510182,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0304-0986","authenticated-orcid":false,"given":"Guoying","family":"Wang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Guangzhou Medical University Department of Hepatobiliary Surgery, , Guangzhou 510120,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7607-4827","authenticated-orcid":false,"given":"Shuai","family":"Mao","sequence":"additional","affiliation":[{"name":"Sun Yat-Sen University Department of Hepatobiliary Surgery, The first affiliated hospital, , Guangzhou 510080,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9003-7733","authenticated-orcid":false,"given":"Weizhong","family":"Li","sequence":"additional","affiliation":[{"name":"Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University School of Medicine, , Shenzhen 518107,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4770-7014","authenticated-orcid":false,"given":"Binghui","family":"Zeng","sequence":"additional","affiliation":[{"name":"Sun Yat-Sen University Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, , Guangzhou 510055,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0454-6935","authenticated-orcid":false,"given":"Wenliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangzhou Medical University The Key Laboratory of Advanced Interdisciplinary Studies, The First Affiliated Hospital of Guangzhou Medical University, GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, , Guangzhou 510182,","place":["China"]},{"name":"The First Affiliated Hospital of Guangzhou Medical University Department of Hepatobiliary Surgery, , Guangzhou 510120,","place":["China"]},{"name":"The University of Hong Kong-Shen Zhen Hospital Department of Pediatrics, , Shenzhen 518053,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,6,9]]},"container-title":["Genomics, Proteomics &amp; 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