{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T05:14:45Z","timestamp":1788239685402,"version":"build-2803163510"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"am","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Bone metastasis can remodel the bone marrow microenvironment, yet how metastatic tumors reshape hematopoiesis and immunity across marrow regions remains poorly defined. Here, we profile a cross-cancer single-cell atlas with 126,986 cells by integrating bone marrow samples (tumor, involved, and distal sites) from liver, prostate, and kidney cancer bone metastases, as well as benign controls, resolving 7 major lineages and 66 subpopulations. We identified coordinated immune suppression and niche remodeling that innate cells rose in peri-tumoral marrow but dropped in tumor sites, while adaptive T\/B cells progressively depleted and stromal\/epithelial compartments expanded. We further revealed a shift in myelopoiesis toward immunosuppressive monocyte\/macrophage states with GMP-level lineage bias, alongside impaired erythropoiesis and B lymphopoiesis driven by myeloid-like reprogramming of precursors. Tumor sites were enriched with exhausted\/stressed T cells, linked to inhibitory progenitor-T cell interactions (e.g., LGALS9-HAVCR2 and CLEC2-KLRB1). Non-hematopoietic support signals (CXCL12-CXCR4, ICAM1-SPN) were attenuated, and tumor programs included a marrow-specific metaprogram with IGFBP3\/NAMPT signaling predicted to further destabilize the HSC niche. Overall, our study offers an integrated framework for decoding the metastatic marrow ecosystem by jointly targeting hematopoietic distortion, stromal collapse, and immune dysfunction.<\/jats:p>","DOI":"10.1093\/gpbjnl\/qzag066","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:21:00Z","timestamp":1788236460000},"source":"Crossref","is-referenced-by-count":0,"title":["Single-cell Atlas Reveals Myelopoietic Bias and Immunosuppressive Reprogramming in Metastatic Bone Marrow Across Cancers"],"prefix":"10.1093","author":[{"given":"Zhilong","family":"Shen","sequence":"first","affiliation":[{"name":"Naval Medical University Department of Orthopedic Oncology, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoxuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Fudan University Shanghai Cancer Center , Institutes of Biomedical Sciences, , Shanghai Key Laboratory of Medical Epigenetics, , Department of Radiation Oncology, , , Shanghai 200032,","place":["China"]},{"name":"State Key Laboratory of Genetic Engineering , Institutes of Biomedical Sciences, , Shanghai Key Laboratory of Medical Epigenetics, , Department of Radiation Oncology, , , Shanghai 200032,","place":["China"]},{"name":"Shanghai Key Laboratory of Radiation Oncology , Institutes of Biomedical Sciences, , Shanghai Key Laboratory of Medical Epigenetics, , Department of Radiation Oncology, , , Shanghai 200032,","place":["China"]},{"name":"Shanghai Medical College , Institutes of Biomedical Sciences, , Shanghai Key Laboratory of Medical Epigenetics, , Department of Radiation Oncology, , , Shanghai 200032,","place":["China"]},{"name":"Fudan University , Institutes of Biomedical Sciences, , Shanghai Key Laboratory of Medical Epigenetics, , Department of Radiation Oncology, , , Shanghai 200032,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Naval Medical University Department of Orthopedic Oncology, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengru","family":"Wang","sequence":"additional","affiliation":[{"name":"Naval Medical University Department of Orthopedic Oncology, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangbin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Naval Medical University Department of Orthopedic Oncology, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Li","sequence":"additional","affiliation":[{"name":"Naval Medical University Department of Orthopedic Oncology, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Yuan","sequence":"additional","affiliation":[{"name":"Naval Medical University Department of Orthopedic Oncology, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangwei","family":"Ni","sequence":"additional","affiliation":[{"name":"Naval Medical University Department of Orthopedic Oncology, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxin","family":"Mao","sequence":"additional","affiliation":[{"name":"Naval Medical University Department of Orthopedic Oncology, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gan","family":"Xu","sequence":"additional","affiliation":[{"name":"Naval Medical University Department of Orthopedic Oncology, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bowen","family":"Lai","sequence":"additional","affiliation":[{"name":"Naval Medical University (Second Military Medical University) Department of Orthopedics, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Naval Medical University Department of Orthopedic Oncology, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhipeng","family":"Zhu","sequence":"additional","affiliation":[{"name":"Naval Medical University Department of Orthopedic Oncology, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tongji University Department of Anesthesiology and Perioperative Medicine, Shanghai Fourth People\u2019s Hospital, School of Medicine, , Shanghai 200434,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congling","family":"Xu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Clinical Research Center for Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Life Sciences and Technology, , Shanghai 200092,","place":["China"]},{"name":"Tongji University Key Laboratory of Anesthesiology and Brain Functional Modulation, Clinical Research Center for Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Life Sciences and Technology, , Shanghai 200092,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianru","family":"Xiao","sequence":"additional","affiliation":[{"name":"Naval Medical University Department of Orthopedic Oncology, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Xu","sequence":"additional","affiliation":[{"name":"Naval Medical University Department of Orthopedic Oncology, Changzheng Hospital, , Shanghai 200003,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,31]]},"container-title":["Genomics, Proteomics &amp; Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/gpb\/advance-article-pdf\/doi\/10.1093\/gpbjnl\/qzag066\/70885994\/qzag066.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/gpb\/advance-article-pdf\/doi\/10.1093\/gpbjnl\/qzag066\/70885994\/qzag066.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:21:01Z","timestamp":1788236461000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/gpb\/advance-article\/doi\/10.1093\/gpbjnl\/qzag066\/8777191"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,31]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/gpbjnl\/qzag066","relation":{},"ISSN":["1672-0229","2210-3244"],"issn-type":[{"value":"1672-0229","type":"print"},{"value":"2210-3244","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,31]]},"article-number":"qzag066"}}