{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T11:18:22Z","timestamp":1790248702258,"version":"4.1.0"},"reference-count":69,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2020,3,30]],"date-time":"2020-03-30T00:00:00Z","timestamp":1585526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"College of Liberal Arts and Science"},{"DOI":"10.13039\/100007482","name":"Arizona State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007482","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Biodesign Institute"},{"name":"Arizona State University and the National Institute of General Medical Sciences","award":["1R01GM118796"],"award-info":[{"award-number":["1R01GM118796"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,27]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Duchenne muscular dystrophy (DMD) is a lethal, X-linked disease characterized by progressive muscle degeneration. The condition is driven by nonsense and missense mutations in the dystrophin gene, leading to instability of the sarcolemma and skeletal muscle necrosis and atrophy. Resulting changes in muscle-specific gene expression that take place in dystrophin\u2019s absence remain largely uncharacterized, as they are potentially obscured by the chronic inflammation elicited by muscle damage in humans. Caenorhabditis elegans possess a mild inflammatory response that is not active in the muscle, and lack a satellite cell equivalent. This allows for the characterization of the transcriptome rearrangements affecting disease progression independently of inflammation and regeneration. In effort to better understand these dynamics, we have isolated and sequenced body muscle-specific transcriptomes from C. elegans lacking functional dystrophin at distinct stages of disease progression. We have identified an upregulation of genes involved in mitochondrial function early in disease progression, and an upregulation of genes related to muscle repair in later stages. Our results suggest that in C. elegans, dystrophin may have a signaling role early in development, and its absence may activate compensatory mechanisms that counteract muscle degradation caused by loss of dystrophin. We have also developed a temperature-based screening method for synthetic paralysis that can be used to rapidly identify genetic partners of dystrophin. Our results allow for the comprehensive identification of transcriptome changes that potentially serve as independent drivers of disease progression and may in turn allow for the identification of new therapeutic targets for the treatment of DMD.<\/jats:p>","DOI":"10.1093\/hmg\/ddaa055","type":"journal-article","created":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T20:10:38Z","timestamp":1585167038000},"page":"1607-1623","source":"Crossref","is-referenced-by-count":14,"title":["Transcriptome changes during the initiation and progression of Duchenne muscular dystrophy in Caenorhabditis elegans"],"prefix":"10.1093","volume":"29","author":[{"given":"Heather C","family":"Hrach","sequence":"first","affiliation":[{"name":"Molecular and Cellular Biology Graduate Program, School of Life Sciences, 427 East Tyler Mall, Tempe, AZ 85287 4501, USA"},{"name":"Virginia G. Piper Center for Personalized Diagnostics, The Biodesign Institute at Arizona State University, 1001 S McAllister Ave, Tempe, AZ 85281, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon","family":"O\u2019Brien","sequence":"first","affiliation":[{"name":"Virginia G. Piper Center for Personalized Diagnostics, The Biodesign Institute at Arizona State University, 1001 S McAllister Ave, Tempe, AZ 85281, USA"},{"name":"Barrett Honors College, Arizona State University, 751 E Lemon Mall, Tempe, AZ 85281, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah S","family":"Steber","sequence":"first","affiliation":[{"name":"Barrett Honors College, Arizona State University, 751 E Lemon Mall, Tempe, AZ 85281, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Newbern","sequence":"first","affiliation":[{"name":"School of Life Sciences, 427 East Tyler Mall, Tempe, AZ 85287 4501, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Rawls","sequence":"first","affiliation":[{"name":"School of Life Sciences, 427 East Tyler Mall, Tempe, AZ 85287 4501, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7551-8793","authenticated-orcid":false,"given":"Marco","family":"Mangone","sequence":"first","affiliation":[{"name":"Virginia G. Piper Center for Personalized Diagnostics, The Biodesign Institute at Arizona State University, 1001 S McAllister Ave, Tempe, AZ 85281, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,3,30]]},"reference":[{"key":"2020062904235104400_ref1","first-page":"498","article-title":"The molecular basis for Duchenne versus Becker muscular dystrophy: correlation of severity with type of deletion","volume":"45","author":"Koenig","year":"1989","journal-title":"Am. J. Hum. 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