{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T10:56:06Z","timestamp":1786272966554,"version":"build-2736575974"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"16","license":[{"start":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T00:00:00Z","timestamp":1595808000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01EY10609"],"award-info":[{"award-number":["R01EY10609"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM12552801A1"],"award-info":[{"award-number":["GM12552801A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Peripherin 2 (PRPH2) is a retina-specific tetraspanin protein essential for the formation of rod and cone photoreceptor outer segments (OS). Patients with mutations in PRPH2 exhibit severe retinal degeneration characterized by vast inter- and intra-familial phenotypic heterogeneity. To help understand contributors to this within-mutation disease variability, we asked whether the PRPH2 binding partner rod OS membrane protein 1 (ROM1) could serve as a phenotypic modifier. We utilized knockin and transgenic mouse models to evaluate the structural, functional and biochemical effects of eliminating one allele of Rom1 (Rom1+\/\u2212) in three different Prph2 models which mimic human disease: C213Y Prph2 (Prph2C\/+), K153Del Prph2 (Prph2K\/+) and R172W (Prph2R172W). Reducing Rom1 in the absence of Prph2 mutations (Rom1+\/\u2212) had no effect on retinal structure or function. However, the effects of reducing Rom1 in the presence of Prph2 mutations were highly variable. Prph2K\/+\/Rom1+\/\u2212 mice had improved rod and cone function compared with Prph2K\/+ as well as amelioration of K153Del-associated defects in PRPH2\/ROM1 oligomerization. In contrast, Prph2R172W\/Rom1+\/\u2212 animals had worsened rod and cone function and exacerbated retinal degeneration compared with Prph2R172W animals. Removing one allele of Rom1 had no effect in Prph2C\/+. Combined, our findings support a role for non-pathogenic ROM1 null variants in contributing to phenotypic variability in mutant PRPH2-associated retinal degeneration. Since the effects of Rom1 reduction are variable, our data suggest that this contribution is specific to the type of Prph2 mutation.<\/jats:p>","DOI":"10.1093\/hmg\/ddaa160","type":"journal-article","created":{"date-parts":[[2020,7,18]],"date-time":"2020-07-18T11:09:03Z","timestamp":1595070543000},"page":"2708-2722","source":"Crossref","is-referenced-by-count":13,"title":["ROM1 contributes to phenotypic heterogeneity in PRPH2-associated retinal disease"],"prefix":"10.1093","volume":"29","author":[{"given":"Daniel","family":"Strayve","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, University of Houston, Houston, TX 77204, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa S","family":"Makia","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of Houston, Houston, TX 77204, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mashal","family":"Kakakhel","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of Houston, Houston, TX 77204, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haarthi","family":"Sakthivel","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of Houston, Houston, TX 77204, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon M","family":"Conley","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA"},{"name":"Oklahoma Center for Neurosciences, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muayyad R","family":"Al-Ubaidi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of Houston, Houston, TX 77204, USA"},{"name":"College of Optometry, University of Houston, Houston, TX 77004, USA"},{"name":"Depatment of Biology and Biochemistry, University of Houston, Houston, TX 77204-5001, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6534-5144","authenticated-orcid":false,"given":"Muna I","family":"Naash","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of Houston, Houston, TX 77204, USA"},{"name":"College of Optometry, University of Houston, Houston, TX 77004, USA"},{"name":"Depatment of Biology and Biochemistry, University of Houston, Houston, TX 77204-5001, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,7,27]]},"reference":[{"key":"2020110419230623500_ref1","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1083\/jcb.116.3.659","article-title":"Localization of peripherin\/rds in the disk membranes of cone and rod photoreceptors: relationship to disk membrane morphogenesis and retinal degeneration","volume":"116","author":"Arikawa","year":"1992","journal-title":"J. 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