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This enzyme synthesizes sphingolipids containing 2-hydroxylated fatty acids, which are particularly abundant in myelin. Fa2h-deficient (Fa2h\u2212\/\u2212) mice develop symptoms reminiscent of the human disease and therefore serve as animal model of SPG35. In order to understand further the pathogenesis of SPG35, we compared the proteome of purified CNS myelin isolated from wild type and Fa2h\u2212\/\u2212 mice at different time points of disease progression using tandem mass tag labeling. Data analysis with a focus on myelin membrane proteins revealed a significant increase of the oligodendrocytic myelin paranodal and inner loop protein (Opalin) in Fa2h\u2212\/\u2212 mice, whereas the concentration of other major myelin proteins was not significantly changed. Western blot analysis revealed an almost 6-fold increase of Opalin in myelin of Fa2h\u2212\/\u2212 mice aged 21\u201323\u00a0months. A concurrent unaltered Opalin gene expression suggested a decreased turnover of the Opalin protein in Fa2h\u2212\/\u2212 mice. Supporting this hypothesis, Opalin protein half-life was reduced significantly when expressed in CHO cells synthesizing 2-hydroxylated sulfatide, compared to cells synthesizing only non-hydroxylated sulfatide. Degradation of Opalin was inhibited by inhibitors of lysosomal degradation but unaffected by proteasome inhibitors. Taken together, these results reveal a new function of 2-hydroxylated sphingolipids namely affecting the turnover of a myelin membrane protein. This may play a role in the pathogenesis of SPG35.<\/jats:p>","DOI":"10.1093\/hmg\/ddaa246","type":"journal-article","created":{"date-parts":[[2020,11,11]],"date-time":"2020-11-11T20:12:17Z","timestamp":1605125537000},"page":"3616-3630","source":"Crossref","is-referenced-by-count":10,"title":["Decreased turnover of the CNS myelin protein Opalin in a mouse model of hereditary spastic paraplegia 35"],"prefix":"10.1093","volume":"29","author":[{"given":"Robert","family":"Hardt","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Bonn 53115, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Jordans","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Bonn 53115, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominic","family":"Winter","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Bonn 53115, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volkmar","family":"Gieselmann","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Bonn 53115, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihua","family":"Wang-Eckhardt","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Bonn 53115, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Eckhardt","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, Bonn 53115, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,11,20]]},"reference":[{"key":"2021012301195813000_ref1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1055\/s-0034-1386767","article-title":"Hereditary spastic paraplegia: clinical principles and genetic advances","volume":"34","author":"Fink","year":"2014","journal-title":"Semin. 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